Basic evaluation toward radioimmuno-detection and-therapy to differentiated thyroid cancer

分化型甲状腺癌放射免疫检测与治疗的基础评价

• 批准号: 05670773
• 负责人: MISAKI Takashi
• 金额: $ 1.34万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670773/
• 关键词: thyroid cancer; thyroglobulin; radioimmunodetection

In an immunostaining study, a human monoclonal antithyro-globulin antibody, VB5, was shown to react the antigen on primary cultures of human thyroid cancer derived from surgical specimens.The antibody was then radiolabeled with I-125, and tested in a inding assay using the same cultured cells as in the staining works. Although measurable, mean specific binding was low at about 1% of the total radioactivity of the ladled antibody in multiple experiments. Therefore, a cocktail of antibodies against various epitopes seemed necessary to perform efficient radioimmunodetection.Despite extensive trials, we failed to establish a cancer cell line which synthesize thyroglobulin. Moreover, two cell lines provided from abroad, though reported to produce the antigen, turned out to be negative in immunostaining. We may need some molecular biological techniques to obtain a transplantable cell line, which will lead to an animal model of differentiated thyroid cancer.

在免疫染色研究中，人抗甲状腺球蛋白单抗VB5与来自手术标本的人甲状腺癌原代培养物发生反应。抗体被I-125放射性标记，并在与染色工作相同的培养细胞中进行结合试验。虽然可以测量，但在多次实验中，平均特异性结合较低，约占包埋抗体总放射性的1%。因此，为了进行有效的放射免疫检测，针对不同表位的抗体的混合似乎是必要的。尽管进行了广泛的试验，我们仍未能建立合成甲状腺球蛋白的癌细胞株。此外，从国外提供的两个细胞株，尽管据报道产生了这种抗原，但免疫染色结果为阴性。我们可能需要一些分子生物学技术来获得可移植的细胞系，这将导致分化型甲状腺癌的动物模型。

项目成果

Misaki T,Alam MS,Sakahara H,Kasagi K and Konishi J.: "Binding of a human monoclonal antithyroglobulin antibody to cultured human thyroid cancer cells." Annals of Nuclear Medicine. Vol.11, No.2. 81-85 (1997)

Misaki T、Alam MS、Sakahara H、Kasagi K 和 Konishi J.：“人单克隆抗甲状腺球蛋白抗体与培养的人甲状腺癌细胞的结合。”

御前 隆: "甲状腺シンチグラム,甲状腺摂取率" 臨床検査. 37. 611-614 (1993)

Takashi Gozen：“甲状腺闪烁图，甲状腺摄取率”临床检查 37. 611-614 (1993)。

御前 隆: "甲状腺中毒症を伴った分化型甲状腺癌の一例" 核医学症例検討会症例集. 16. 7-8 (1993)

Takashi Gozen：“伴有甲状腺毒症的分化型甲状腺癌一例”核医学病例研究组病例集。16. 7-8 (1993)。

T, Misaki: "Binding of a human manoclonal antithyroglobulin antilbody to cultured human thyroid cancer cells" Annals of Nuclear Medicine. 11. 81-85 (1997)

T，Misaki：“人单克隆抗甲状腺球蛋白抗体与培养的人甲状腺癌细胞的结合”核医学年鉴。

御前隆: "甲状腺シンチグラフィ、甲状腺摂取率" カレントテラピー. 13. 30-33 (1995)

Takashi Ozen：“甲状腺闪烁扫描，甲状腺摄取率”《当前治疗》13. 30-33 (1995)。