Basic evaluation toward radioimmuno-detection and-therapy to differentiated thyroid cancer

在一项免疫染色研究中，一种人单克隆抗甲状腺球蛋白抗体VB5在手术标本中提取的人甲状腺癌原代培养物上与抗原发生反应。然后用I-125对抗体进行放射性标记，并使用与染色作品中相同的培养细胞进行检测。虽然可测量，但在多次实验中，平均特异性结合较低，约为包埋抗体总放射性的1%。因此，针对各种表位的抗体鸡尾酒似乎是进行有效放射免疫检测所必需的。尽管进行了大量的试验，我们还是未能建立一种能够合成甲状腺球蛋白的癌细胞系。此外，从国外提供的两株细胞株，虽然报道产生抗原，但免疫染色结果为阴性。我们可能需要一些分子生物学技术来获得可移植的细胞系，这将导致分化甲状腺癌的动物模型。

In an immunostaining study, a human monoclonal antithyro-globulin antibody, VB5, was shown to react the antigen on primary cultures of human thyroid cancer derived from surgical specimens.The antibody was then radiolabeled with I-125, and tested in a inding assay using the same cultured cells as in the staining works. Although measurable, mean specific binding was low at about 1% of the total radioactivity of the ladled antibody in multiple experiments. Therefore, a cocktail of antibodies against various epitopes seemed necessary to perform efficient radioimmunodetection.Despite extensive trials, we failed to establish a cancer cell line which synthesize thyroglobulin. Moreover, two cell lines provided from abroad, though reported to produce the antigen, turned out to be negative in immunostaining. We may need some molecular biological techniques to obtain a transplantable cell line, which will lead to an animal model of differentiated thyroid cancer.