Study on the histological occurrence of UC-associated cancer and dysplasia

UC相关癌症和不典型增生的组织学发生研究

• 批准号: 05671046
• 负责人: SAWADA Toshio
• 金额: $ 1.22万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671046/
• 关键词: Ulcerative colitis; Colorectal cancer; dysplasia; p53; K-ras; 癌; Two-step RFLP法; P53; ELISA法; 大腸腺腫

Ulcerative colitis (UC) associated cancer or dysplasia is characteristic as follows : (1) The morphological status is plaque, (2) Multiple lesions occur, (3) One lesion has another lesion of different atypia around it. Generally the character of a cancer originates from genetic change. Accordingly it is expected that the genetic changes are different between sporadic colorectal cancers and UC-associated cancers.In the study in 1993, we analyzed K-ras mutation. The frequency of K-ras mutation was 60-70% in sporadic colorectal cancers and adenomas. But that was about 10% in UC-associated cancers or dysplasias.In the study in 1994, we analyzed p53 abnormality. We investigated immunohistochemically p53 protein expression of the specimens in paraffin blocks. p53 mutation correlates with p53 protein overexpression. Our data showed p53 protein overexpression was 89% in invasive carcinoma, 70% in high-grade dysplasia, 57% in low-grade dysplasia, but 0% in adenoma, indefinite for dysplasia and nigative for dysplasia. These data suggest that p53 expression is an objective marker not only to confirm a tumor but also to differ adenoma from dysplasia indicative of invasive carcinoma.

溃疡性结肠炎（UC）相关的肿瘤或发育不良具有以下特点：(1)形态为斑块；(2)多发病变；(3)一个病变周围有另一个不同异型的病变。一般来说，癌症的特征源于基因变化。因此，预计散发性结直肠癌和uc相关癌症之间的遗传变化是不同的。在1993年的研究中，我们分析了K-ras突变。散发性结直肠癌和腺瘤中K-ras突变的发生率为60-70%。但在uc相关的癌症或发育不良中，这一比例约为10%。在1994年的研究中，我们分析了p53的异常。我们用免疫组织化学方法检测了p53蛋白在石蜡块中的表达。P53突变与P53蛋白过表达相关。我们的数据显示，p53蛋白过表达在浸润性癌中为89%，在高级别非典型增生中为70%，在低级别非典型增生中为57%，在腺瘤中为0%，在非典型增生中不确定，在非典型增生中为阴性。这些数据表明，p53的表达不仅是确认肿瘤的客观标志，而且是区分腺瘤和浸润性癌的异常增生的标志。

项目成果

篠崎大: "炎症性大腸疾患の現況と将来" 消火器外科. 18. 275-284 (1995)

Dai Shinozaki：“炎症性肠病的现状和未来”，灭火器外科，18. 275-284 (1995)。

Masaru Shinozaki: "Colitic cancer with speeial reference to its shape and surface pattern, Report of Two Cases" I to Cho. 30. 671-676 (1995)

Masaru Shinozaki：“结肠癌，特别参考其形状和表面图案，两个病例的报告”我给 Cho。

篠崎 大: "潰瘍性大腸炎合併大腸癌の2例" 胃と腸. 30. 1001-1006 (1995)

Dai Shinozaki：“与溃疡性结肠炎相关的结肠癌两例”《胃与肠》30. 1001-1006 (1995)。

Masaru Shinozaki: "A case report of Villous descending colon cancer associated with ulcerative colitis" Japanese Journal of Gastroenterology. 91. 1973-1978 (1994)

Masaru Shinozaki：“与溃疡性结肠炎相关的绒毛状降结肠癌病例报告”日本胃肠病学杂志。

鈴木公孝: "潰瘍性大腸炎における大腸癌の合併とその早期診断" 消化器外科. 16(4). 443-451 (1993)

Kimitaka Suzuki：“溃疡性结肠炎的结直肠癌并发症及其早期诊断”胃肠外科16(4)。