Hypothalamic hormones and central neuronal regulation following

1)伤害性刺激后即刻早期基因(IEG)和多肽mRNAs的表达我们观察了下丘脑、脊索和脊神经节中c-fos和c-jun基因的表达。观察实验后1、8、24、48小时的变化。在所有受检区，c-fos和c-jun的mRNAs在1小时和2小时后均升高。在下丘脑中，CRF、加压素和脑啡肽的mRNAs在损伤后增加。由于c-fos和加压素(或CRF)的mRNAs是共存的，所以在IEGs的表达过程中伴随着肽mRNA的变化。2)在伤害性刺激后，我们观察到NGF诱导的VGF在下丘脑室旁核的诱导。由于已知VGF的诱导代表神经元的活动，因此本结果提示伤害性刺激后下丘脑室旁神经元的活动增加。3)盐负荷的复水过程为了研究应激的恢复过程，我们用免疫细胞化学的方法检测了下丘脑肽能神经元的恢复情况。我们发现血管加压素和甘丙肽在复水1周后恢复正常，但强啡肽即使在1周后仍然很低。这些发现表明，压力诱导的内分泌变化在大脑中即使在1周后仍然存在。

1) Immediate early genes (IEG) and peptide mRNAs following nociceptive stimulusFollowing the injection of formaline into the lower pads or avulsion of sciatic nerve, we observed the c-fos and c-jun mRNAs in hypothalamus, spinal chord, and spinal ganglion. We observed the changes 1,8,24, and 48 hours following these experiments. In all regions examined, c-fos and c-jun mRNAs were increased after 1 and 2 hours later. In the hypothalamus, CRF,vasopressin and enkephalin mRNAs were increased following the injury. Since c-fos and vasopressin (or CRF) mRNAs were coexiswted, the change of peptide mRNA accompanies the expression of IEGs.2) Induction of VGF following the nociceptive stimulusFollowing the avulsion of sciatic nerve, we observed the induction of VGF,a NGF-inducible peptide, in the paraventricular nucleus of the hypothalamus. Since it is known that the induction of VGF represents the activity of neurons, the present result suggests the activity of hypothalamic paraventricular neurons increased after the nociceptive stimulus.3) Rehydration prrocess from salt-loadingFor examining the recovery process of stress, we examined the recovery of hypothalamic peptidergic neurons by the immunocytochemical method. We found that vasopressin and galanin returned to normal after 1 week of the rehydration, but dynorphin remained low even 1 week later. These findings suggests that the stress-induced endocrine change remains even after 1 week in the brain.