Development of a Predictive Assay for Radiosensitivity of Esophageal and Head and Neck Cancers and Modification of the Sensitivity Using Anti-c-erbB 2 Compounds

我们正在开发预测食道癌和头颈癌放射治疗结果的方法。我们回顾分析了食道癌患者的治疗结果。我们还检测了抗c-erbB-2单抗曲妥珠单抗以及其他化合物对6种食管鳞癌细胞系放射敏感性的影响。细胞表面蛋白的表达与临床预后无明显关系。放射治疗的严重不良反应，如心脏增大或心力衰竭，仅与照射野的大小有关。在体外分析中，我们不能证明曲妥珠单抗或包括AG1478在内的其他2c-erbB-2抑制剂有任何放射增敏作用。为了开发一种改进的辐射效应预测方法，我们对6种不同的食管鳞状细胞癌细胞进行了微核试验。然而，我们没有检测到照射后的微核数量与癌细胞的辐射敏感性之间有任何显著的关系。

We are developing predictive methods for results of radiation therapy for esophageal and head and neck cancers. We retrospectively analyzed treatment outcomes for patients with esophageal cancer. We also tested the effects of trastuzumab, an anti-c-erbB-2 monoclonal antibody, as well as other compounds on the radiosensitivity of 6 esophageal squamous cell carcinoma cell lines.The retrospective analysis consisted of 68 patients with esophageal squamous cell carcinoma. There was no significant relation between expression of cell surface proteins and the clinical outcome. Severe adverse effect of radiation therapy, such as cardiomegaly or heart failure, was related solely related to the size of the radiation field.On in vitro analysis, we could not demonstrate any radiosensitizing effects of trastuzumab or other 2 c-erbB-2 inhibitors including AG1478. To develop an improved predictive method of the effects of radiation, we tested the usefulness of micronucleus assay in 6 different esophageal squamous cell carcinoma cells. However, We could not detect any significant relationship between the number of micronuclei after irradiation and the radiosensitivity of the cancer cells.