Development of a Predictive Assay for Radiosensitivity of Esophageal and Head and Neck Cancers and Modification of the Sensitivity Using Anti-c-erbB 2 Compounds

食管癌和头颈癌放射敏感性预测分析的开发以及使用抗 c-erbB 2 化合物修改敏感性

• 批准号: 16390335
• 负责人: SASAI Keisuke
• 金额: $ 4.74万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390335/
• 关键词: Radiation therapy; Radiosensitivity; Esophageal cancer; Head and neck cancer; c-erbB-2; EGFR; Micronucleus assay; AG1478; EGFRファミリー; トラスツズマブ; c-erbB2; 放射線増感

We are developing predictive methods for results of radiation therapy for esophageal and head and neck cancers. We retrospectively analyzed treatment outcomes for patients with esophageal cancer. We also tested the effects of trastuzumab, an anti-c-erbB-2 monoclonal antibody, as well as other compounds on the radiosensitivity of 6 esophageal squamous cell carcinoma cell lines.The retrospective analysis consisted of 68 patients with esophageal squamous cell carcinoma. There was no significant relation between expression of cell surface proteins and the clinical outcome. Severe adverse effect of radiation therapy, such as cardiomegaly or heart failure, was related solely related to the size of the radiation field.On in vitro analysis, we could not demonstrate any radiosensitizing effects of trastuzumab or other 2 c-erbB-2 inhibitors including AG1478. To develop an improved predictive method of the effects of radiation, we tested the usefulness of micronucleus assay in 6 different esophageal squamous cell carcinoma cells. However, We could not detect any significant relationship between the number of micronuclei after irradiation and the radiosensitivity of the cancer cells.

我们正在开发食管癌和头颈癌放射治疗结果的预测方法。我们回顾性分析了食管癌患者的治疗结果。我们还检测了抗c-erbB-2单克隆抗体曲妥珠单抗（trastuzumab）及其他化合物对6株食管鳞癌细胞系放射敏感性的影响。细胞表面蛋白的表达与临床预后无明显关系。放射治疗的严重不良反应，如心脏肥大或心力衰竭，仅与照射野的大小有关，在体外分析中，我们不能证明曲妥珠单抗或其他2种c-erbB-2抑制剂包括AG 1478的任何放射增敏作用。为了建立一种改进的预测辐射效应的方法，我们在6种不同的食管鳞癌细胞中测试了微核试验的有用性。然而，我们没有发现照射后的微核数与癌细胞的放射敏感性之间有任何明显的关系。

项目成果

Long-term results of chemoradiotherapy for locally advanced esophageal cancer, using daily low-dose 5-fuluorouracil and cis-diammine-dichloroplatinum (CDDP)

使用每日低剂量 5-氟尿嘧啶和顺式二氨二氯铂 (CDDP) 进行局部晚期食管癌放化疗的长期结果

• 发表时间: 2007
• 期刊: International Journal of Clinical Oncology 12・1
• 作者: Sasamoto R;Tsuchida E;Ito;Yamana N;Sasai K 他
• 通讯作者: Sasai K 他

Long-term results of chemoradiotherapy for locally advanced esophageal cancer, using daily low-dose 5-fuluorouracil and cisdiammine-dichloroplatinum (CDDP).

使用每日低剂量 5-氟尿嘧啶和顺二氨二氯铂 (CDDP) 治疗局部晚期食管癌的长期结果。

• 发表时间: 2007
• 期刊: Int J Clin Oncol. 12(1)
• 作者: Sasamoto R;Sakai K;Inakoshi H;Sueyama H;Saito M;Sugita T;Tsuchida E;Ito T;Matsumoto Y;Yamanoi T;Abe E;Yamana N;Sasai K.
• 通讯作者: Sasai K.

Long-term results of chemoradiotherapy for locally advanced esophageal cancer, using daily low-dose 5-fuluorouracil and cis-diamine-dichloroplatinum(CDDP)

每日低剂量 5-氟尿嘧啶和顺式二胺二氯铂 (CDDP) 局部晚期食管癌放化疗的长期结果

• 发表时间: 2007
• 期刊: International Journal of Clinical Oncology 12・1
• 作者: Sasamoto R;Tsuchida E;Ito;Yamana N;Sasai K 他
• 通讯作者: Sasai K 他

Risk factors for enlargement of cardiac silhouette on chest radiography after radiotherapy for esophageal cancer.

• DOI: 10.1007/s11604-006-0050-1
• 发表时间: 2006-07-01
• 期刊: Radiation medicine
• 作者: Sasamoto, Ryuta;Tsuchida, Emiko;Sasai, Keisuke
• 通讯作者: Sasai, Keisuke

Concurrent chemoradiotherapy using low-dose continuous infusion of 5-fluorouracil for postoperative regional lymph node recurrence of esophageal squamous cell carcinoma.

小剂量持续输注5-氟尿嘧啶同步放化疗治疗食管鳞癌术后区域淋巴结复发。

• 发表时间: 2005
• 期刊: Esophagus 2・1
• 作者: Tuschida E;Sakai K;Matsumoto Y;Sugita T;Sasamoto R;Yamanoi T;Sueyama H;Ito T;Sasai K
• 通讯作者: Sasai K