Cytokeratin expression and CSF in cholesteatoma

胆脂瘤中细胞角蛋白的表达和脑脊液

• 批准号: 05671439
• 负责人: KITAMURA Ken
• 金额: $ 1.47万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671439/
• 关键词: Cholesteatoma; Cytokeratin; Immunohistochemistry; Electrophoresis; 電気泳動; 真珠腫; CSF; Wseternブロッティング; 抗ヒトサイトケラチン抗体; ミニブタ; G-CSF; M-CSF

Expression patterns of cytokeratins (CKs) in normal skin, in pars flaccida type cholesteatoma (PFTC), and in pars tensa type cholesteatoma (PTTC) were examined by means of one-and two-dimensional electrophoretic techniques. Both CKs 14 and 5 pair (CKs 14/5) and CKs 10/1 were found in all materials. Neither CKs 16/6 nor 19 was found in the skin. CKs 16/6 and 19 were both found in 3 out of 5 PFTCs, only CKs 16/6 in 1 out of 5 and neither CKs 16/6 nor 19 in 1 out of 5. CKs 16/6 and 19 were both found in 1 out of 3 PTTCs, only Cks 16/6 in 1 out of 3 and neither CKs 16/6 nor 19 in 1 out of 3. There was no significant difference in the CKs expression patterns between PFTC and PTTC.The expression of Cks 16/6 and 19 suggested that their matrix epithelia were hyperproliferative. However, not all the cholesteatomas were always hyperproliferative. Patterns of the terminal differentiation of CKs 1,5,10 and 14 in the PFTC or the PTTC were basicaliy the same as those in the skin. In the cholestatoma, eack CK diminished gradually in molecular weight in the cornified layr and debris. Desmosomal proteins were abundant in skin but not in cholesteatomas.

应用一维和二维电泳技术研究了细胞角蛋白（CKs）在正常皮肤、松弛部型胆脂瘤（PFTC）和紧张部型胆脂瘤（PTTC）中的表达模式。CKs 14和5对（CKs 14/5）和CKs 10/1在所有材料中均存在。CKs 16/6和CKs 19在皮肤中均未发现。3/5例PFTC同时检出CK 16/6和19，1/5例仅检出CK 16/6，1/5例既未检出CK 16/6也未检出CK 19。1/3例PTTC同时检测到CKs 16/6和19，1/3例仅检测到CKs 16/6，1/3例未检测到CKs 16/6和19。PFTC与PTTC间CKs表达无显著性差异，CKs 16/6和19的表达提示其基质上皮细胞过度增生。然而，并不是所有的中耳瘤都是过度增殖的。CKs 1、5、10和14在PFTC和PTTC中的终末分化模式与皮肤中的基本相同。在胆脂瘤中，皮质层和碎片中的CK分子量逐渐减少。桥粒蛋白在皮肤中丰富，但在中耳瘤中不丰富。

项目成果

Kakoi,Hiroyuki: "Cytokeratin expression patterns by one-and two- dimensional electrophoresisin pars flaccida cholesteatoma and pars tensa cholesteatoma." Acta Otolaryngol(Stockh). 115. 804-810 (1995)

Kakoi，Hiroyuki：“松弛部胆脂瘤和紧张部胆脂瘤的一维和二维电泳的细胞角蛋白表达模式。”

Kakoi, Hiroyuki: "Cytokeratin expression patterns by one-and two-dimensional electrophoresis in pars flaccida cholesteatoma and pars tensa cholesteatoma." Acta Otolaryngol (Stockh). 115. 804-810 (1995)

Kakoi、Hiroyuki：“通过一维和二维电泳观察松弛部胆脂瘤和紧张部胆脂瘤中的细胞角蛋白表达模式。”