ISOLATION AND SOME PROPERTIES OF ADHERENT MATERIALS OF PORPHYROMONAS GINGIVALIS

牙龈卟啉单胞菌与牙周组织细胞表面的粘附可能在其致病性中起重要作用。在这项研究中，我们选择了一些菌株，包括侵入性菌株和非侵入性菌株，研究了牙龈韧带成分的粘附性。试验菌株对胶原蛋白、纤维连接蛋白和层粘连蛋白的粘附程度有显著差异。总体上，疏水性与附着在羟基磷灰石珠上的胶原蛋白上的细胞数量呈正相关。从非侵入性菌株提取的菌毛与胶原蛋白结合较强，而从侵入性菌株提取的菌毛与胶原蛋白或唾液包覆的羟基磷灰石结合较弱。我们还从牙龈卟啉单胞菌中提取了囊泡黏附素并进行了表征。用CHAPS法、凝胶过滤法和精氨酸亲和法提取囊泡黏附素。SDS-PAGE检测到52 kDa蛋白，对血凝素反应较弱，对胰蛋白酶反应不明显。结果表明：1)试验菌株的菌毛与牙龈组织或膜的黏附程度有显著差异；2)囊泡黏附素在侵袭性菌株和非侵袭性菌株中均存在，其特征蛋白为52kD；3)该黏附素具有较弱的血凝素。

The adherence of Porphyromonas gingivalis to cell surfaces of periodontal tissues may play an important role in its pathogenicity. In this study, some selected strains of P.gingivalis, including both invasive and non invasive strains, were investigated for adherence to gingival ligament components. The test strains of P.gingivalis adhered to collagen, fibronectin and laminin to significantly different degrees. An overall positive correlation was noted between hydrophobicity and the number of cells that attached to collagen coated on hydroxyapatite beads. Fimbriae extracted from non invasive strains bound strongly to collagen, whereas invasive strains'fimbriae bound weakly to collagen or saliva coated hydroxyapatite.. We have also extracted to vesicle adhesin from Porphyromonas gingivalis and characterized. The adhesin of vesicle was extracted with the CHAPS,gel-filtration, and affinity with aruginine.Protein of 52 kDa was observed in SDS-PAGE,and indicated to hemagglutinin weakly, not trypsin-activity. These data suggest that 1) the test strains'fimbriae adhered to gingival tissue or pellicle to significantly different degrees ; 2) adhesin from vesicle was common to invasive and non invasive strains and was characterized to protein of 52kD ; 3) this adhesin has hemagglutinin weakly.