Attachment and invasion of periodontopathic bacteria tosubgingival site

牙龈拟杆菌与人类成人牙周炎有关。这种生物已经被证明附着在类似牙齿的矿物表面形成的实验性唾液膜上。近年来，研究人员发现唾液中富含脯氨酸的酸性蛋白（PRP）可促进牙龈芽胞杆菌对羟基磷灰石表面的附着。这些唾液蛋白与各种类型的胶原蛋白有一些共同的肽序列。由于胶原蛋白是牙周组织的主要成分，在牙周病的过程中被破坏，我们研究了牙龈芽孢杆菌细胞与胶原基质结合的能力。用1型或4型人胶原蛋白（C-HA）处理HA可显著促进牙龈芽孢杆菌细胞的附着。加热牙龈b细胞破坏了它们附着1型C-HA的能力。不同菌株的牙龈芽孢杆菌粘附C-HA的能力不同。2.人IgG、纤维连接蛋白、纤维蛋白原、人血清和一些肽如gly-gln、gly-asp、前gln-gly抑制C-HA的附着。3.疏水性与它们附着在C-HA上的数量之间存在总体相关性。4.经纤维连接和层粘连蛋白处理的牛骨胶原减少了牙龈B. 33277细胞和其他附着C-HA的细胞数量，而W83细胞通过纤维连接蛋白附着C-HA的数量增加。5.表面有许多膜的菌株与C-HA有很大的结合，而一些表面有少量膜的菌株与C-HA没有结合。

Bacteroides gingivalis has been associated with adult periodontitis in humans. The organism have been shown to attach to experimental saliva pellicles formed on mineral surfaces similar to those to teeth. Recently, adsorbed acidic saliva proline-rich proteins (PRP) have been found to promote the attachment of B.gingivalis cells to hydroxyapatite surfaces. These salivary proteins have some peptide sequences in common with various types of collagen. Since collagen is a major component of the periodontium, and is destroyed during the course of periodontal disease, we investigated the ability of B. gingivalis cells to bind to collagenous substrata.1.Treating HA with either type 1 or type 4 human collagen(C-HA) greatly promoted attachment of B.gingivalis cells.Heating B. gingivalis cells destroyed their ability to attach to type 1 C-HA.The individual strains of B. gingivalis differ in their ability to adhere to C-HA. 2.Human IgG, fibronectin, fibrinogen, human serum and some peptide i.e. gly-gln, gly-asp, pro-gln-gly inhibited attachment to C-HA. 3.An overall correlation was noted between the hydrophobicity and their numbers which attached to C-HA. 4.Pretreatment of bovine bone collagen with fibronection and laminin decreased the number of B. gingivalis 33277 cells and the other cells which attached C-HA, however W83 cells were increased to attach to the C-HA with fibronectin. 5.The bacterial strain which have a number of fimbraie on their surface, greatly attached to C-HA, while some B. ginjivalis strains having a few fimbrae were not binding to C-HA.