A basic study on the development of diagnostic and treatment for periodontal diseases using monoclonal antibodies against human periodonopathic bacteria.

牙龈拟杆菌已被确认为引起成人牙周炎。在我们的实验室中已经报道了成年牙周炎患者的抗体升高对微生物浓度的反应。本研究是为了确定哪些表面抗原是有用的检测血清抗体的微生物与单克隆抗体的免疫印迹分析。几乎所有患者血清均与超声抗原、玻璃珠提取抗原和细胞粘附因子（血凝素）反应。成人牙周炎患者血清中大多数与血凝素呈强阳性反应，但抗脂多糖（LPS）和荚膜抗原的抗体水平差异较大，抗B的特异性IgG水平较低。牙龈炎通过微量ELISA测定。抗抗原IgG水平与牙槽骨丢失程度呈统计学正相关。另一方面，没有发现针对抗原的抗体水平和袋深度之间的显著相关性。从患者获得的血清与血凝素反应，证实了免疫印迹试验。健康人血清与这些抗原均无反应，而成人牙周炎患者血清中大多数对血凝素有较高的抗体水平。这一结果表明，粘附性表面结构如血凝素作为第一步参与了B。牙龈定植和抗原刺激。目前的研究结果强调使用血凝素检测抗体对B.牙龈在成人牙周炎的初始阶段。

Bacteroides gingivalis has been recognized as causing adult periodontitis. Antibody elevations in adult patients with periodontitis in response to the concentration of the organisms have been reported in our labolatory. The present research was done to determine which surface antigens was useful in detecting serum antibody to the microorganisms by immunoblotting analysis with monoclonal antibodies. Almost all sera obtained from the patients reacted with sonicate antigen, glass-beads extracted antige, and attachment factor(hemagglutinin) of the cells. Most sera from adult periodontitis sera reacted strongly with hemagglutinin, but a considerable variation in antibody levels against lipopolysaccharide(LPS) and capsular antigen was found. The specific IgG level to B. gingivalis was determined by a micro ELISA. A statistically positive correlation was found between IgG levels against the antigens and the degree of alveolar bone loss. On the other hand, no significant correlation was found between the antibody levels against the antigens and pocket depth. The sera obtained from patients reacted with hemagglutinin were confirmed by the immunoblotting tests. Sera from healthy individuals did not react with these antigens.It is interesting that most sera from patients with adult periodontitis possessed high antibody levels to hemagglutinin. This result indicates that addhessive surface structures such as hemagglutinin participate as a first step in B. gingivalis colonization and antigenic stimulation. The present findings emphasize the use of hemagglutinin to detect antibodies against B.gingivalis during the initial stages of adult periodontitis.