A basic study on the development of diagnostic and treatment for periodontal diseases using monoclonal antibodies against human periodonopathic bacteria.

牙龈拟杆菌已被认为是引起成人牙周炎的原因。抗体升高在成人牙周炎患者响应的浓度的生物体已报告在我们的实验室。本研究通过单克隆抗体免疫印迹分析，确定哪些表面抗原可用于检测血清对微生物的抗体。几乎所有患者的血清都与超声抗原、玻璃珠提取抗原和细胞的附着因子（血凝素）反应。大多数成人牙周炎血清与血凝素反应强烈，但对脂多糖（LPS）和荚膜抗原的抗体水平有相当大的差异。采用微量ELISA法测定血清对牙龈白杆菌的特异性IgG水平。抗抗原IgG水平与牙槽骨丢失程度呈正相关。另一方面，抗抗原抗体水平与口袋深度之间没有显著相关性。免疫印迹试验证实了与血凝素反应的患者血清。健康人的血清对这些抗原没有反应。有趣的是，大多数成人牙周炎患者的血清对血凝素具有高抗体水平。这一结果表明，黏附的表面结构，如血凝素，作为牙龈芽孢杆菌定植和抗原刺激的第一步参与。目前的研究结果强调在成人牙周炎的初始阶段使用血凝素检测抗牙龈杆菌抗体。

Bacteroides gingivalis has been recognized as causing adult periodontitis. Antibody elevations in adult patients with periodontitis in response to the concentration of the organisms have been reported in our labolatory. The present research was done to determine which surface antigens was useful in detecting serum antibody to the microorganisms by immunoblotting analysis with monoclonal antibodies. Almost all sera obtained from the patients reacted with sonicate antigen, glass-beads extracted antige, and attachment factor(hemagglutinin) of the cells. Most sera from adult periodontitis sera reacted strongly with hemagglutinin, but a considerable variation in antibody levels against lipopolysaccharide(LPS) and capsular antigen was found. The specific IgG level to B. gingivalis was determined by a micro ELISA. A statistically positive correlation was found between IgG levels against the antigens and the degree of alveolar bone loss. On the other hand, no significant correlation was found between the antibody levels against the antigens and pocket depth. The sera obtained from patients reacted with hemagglutinin were confirmed by the immunoblotting tests. Sera from healthy individuals did not react with these antigens.It is interesting that most sera from patients with adult periodontitis possessed high antibody levels to hemagglutinin. This result indicates that addhessive surface structures such as hemagglutinin participate as a first step in B. gingivalis colonization and antigenic stimulation. The present findings emphasize the use of hemagglutinin to detect antibodies against B.gingivalis during the initial stages of adult periodontitis.