A basic study on the development of diagnostic and treatment for periodontal diseases using monoclonal antibodies against human periodonopathic bacteria.
A basic study on the development of diagnostic and treatment for periodontal diseases using monoclonal antibodies against human periodonopathic bacteria.
批准号:
60570879
负责人:
NAITO Yuko
金额:
$1.15万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1985
资助国家:
日本
项目状态:
已结题
起止时间:
1985 至 1986
中文摘要
牙龈拟杆菌已被确认为引起成人牙周炎。在我们的实验室中已经报道了成年牙周炎患者的抗体升高对微生物浓度的反应。本研究是为了确定哪些表面抗原是有用的检测血清抗体的微生物与单克隆抗体的免疫印迹分析。几乎所有患者血清均与超声抗原、玻璃珠提取抗原和细胞粘附因子(血凝素)反应。成人牙周炎患者血清中大多数与血凝素呈强阳性反应,但抗脂多糖(LPS)和荚膜抗原的抗体水平差异较大,抗B的特异性IgG水平较低。牙龈炎通过微量ELISA测定。抗抗原IgG水平与牙槽骨丢失程度呈统计学正相关。另一方面,没有发现针对抗原的抗体水平和袋深度之间的显著相关性。从患者获得的血清与血凝素反应,证实了免疫印迹试验。健康人血清与这些抗原均无反应,而成人牙周炎患者血清中大多数对血凝素有较高的抗体水平。这一结果表明,粘附性表面结构如血凝素作为第一步参与了B。牙龈定植和抗原刺激。目前的研究结果强调使用血凝素检测抗体对B.牙龈在成人牙周炎的初始阶段。
英文摘要
Bacteroides gingivalis has been recognized as causing adult periodontitis. Antibody elevations in adult patients with periodontitis in response to the concentration of the organisms have been reported in our labolatory. The present research was done to determine which surface antigens was useful in detecting serum antibody to the microorganisms by immunoblotting analysis with monoclonal antibodies. Almost all sera obtained from the patients reacted with sonicate antigen, glass-beads extracted antige, and attachment factor(hemagglutinin) of the cells. Most sera from adult periodontitis sera reacted strongly with hemagglutinin, but a considerable variation in antibody levels against lipopolysaccharide(LPS) and capsular antigen was found. The specific IgG level to B. gingivalis was determined by a micro ELISA. A statistically positive correlation was found between IgG levels against the antigens and the degree of alveolar bone loss. On the other hand, no significant correlation was found between the antibody levels against the antigens and pocket depth. The sera obtained from patients reacted with hemagglutinin were confirmed by the immunoblotting tests. Sera from healthy individuals did not react with these antigens.It is interesting that most sera from patients with adult periodontitis possessed high antibody levels to hemagglutinin. This result indicates that addhessive surface structures such as hemagglutinin participate as a first step in B. gingivalis colonization and antigenic stimulation. The present findings emphasize the use of hemagglutinin to detect antibodies against B.gingivalis during the initial stages of adult periodontitis.
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Naito,Y;Okuda;K;Kato,T;Takazoe,I: Infection and Immunity. 50. 231-235 (1985)
Naito,Y;Okuda;K;Kato,T;Takazoe,I:感染与免疫。
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通讯作者:
Takazoe,I;Okuda,K.: "Pathogenic of black-pigmented Bacteroides in periodontal disease.Proceedings of 2nd International Symposium in Anaerobes." Japan Association for Anaerobic Infection for Research, 205-217 (1986)
Takazoe,I;Okuda,K.:“牙周病中黑色拟杆菌的致病性。第二届国际厌氧菌研讨会论文集。”
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Okuda,K;Kato,T;Naito,Y.;Takazoe,I.: Journal of clinical Microbiology. 24. 846-848 (1986)
奥田,K;加藤,T;内藤,Y.;高添,I.:临床微生物学杂志。
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通讯作者:
Takazoe,I;Okuda,K.: "Immunology of periodontal disease.Proceedings in 【VIII】 International Conference of Labeled Antibodies." Tokyo University Publisher,
Takazoe, I; Okuda, K.:“牙周病的免疫学。[VIII]国际标记抗体会议论文集”,
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Okuda,K;Ohta,K;Kato,T.Takazoe,I;Slots,J.: Journal of Clinical Microbiology. 24. 89-95 (1986)
奥田,K;太田,K;加藤,T.Takazoe,I;Slots,J.:临床微生物学杂志。
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共 14 条
Elucidation of regulatory mechanism of B cell activation by the change in sialic acid species
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ISOLATION AND SOME PROPERTIES OF ADHERENT MATERIALS OF PORPHYROMONAS GINGIVALIS
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The heterogeneity of various Porphyromonas gingivalis strains to adherence and invasive.
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财政年份:1990
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依托单位:
Attachment and invasion of periodontopathic bacteria tosubgingival site
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批准号:63570878
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.34万
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财政年份:1988
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负责人:NAITO Yuko
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依托单位:
海外基金