Detection of periodontal disease activity by T cell receptor repertoire and cytokine profile of infiltrating T cells

通过 T 细胞受体库和浸润 T 细胞的细胞因子谱检测牙周病活动

• 批准号: 05671593
• 负责人: YAMAZAKI Kazuhisa
• 金额: $ 1.41万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671593/
• 关键词: Periodontitis; Gingivitis; Functional subsets; Th1; Th2; T cell receptor; Immunohistochemistry; IL-4; IL-6

In order to characterize the T cells infiltrating gingival tissues with periodontitis, T cell receptor repertoire and cytokine profile were investigated by means of immunohistological procedure. Periodontitis tissues were obtained at the time of periodontal surgery after completion of initial therapy. Gingivitis tissues as a control were also obtained from non-periodontitis lesions. Serial cryostat sections from each specimen were immunostained for TCR V gene products (Valpha2, Vbeta5.2-3, Vbeta5.3, Vbeta5.1, Vbeta6.7, Vbeta8, Vbeta12) and cytokines (IL-2, IFN-gamma, IL-4, IL-6) with alkaline phosphataseanti alkaline phosphatase method. Relative percentages were caluclated by dividing the number of each positive cell by total number of CD3 positive cells. Moreover, for comparison between gingival tissues and circulating pool, peripheral blood mononuclear cells from both groups were analyzed for the TCR repertoire by flow cytometry. The pattern of each TCR V gene product expression in i … More nflamed gingiva exhibited individual variation, nevertheless a consistent pattern emerged. The Vbeta5 subfamily and Vbeta6.7 were frequently used repertoires in gingiva, whereas the Valpha2 and Vbeta8 subfamily were underexpressed in most cases. Furthermore, the TCR V gene product expression in gingival tissue was biased compared with autologous peripheral blood. Three out of 10 periodontitis subjects showed 1 or 2 strikingly overrepresented repertoire comparatively with autologous blood. In these 3 subjects Vbeta6.7 was overexpressed in two cases and Vbeta5.2-3, Vbeta8 and Vbeta12 were overexpressed in one case. With respect to the cytokine profile, the proportion of IL-4- and IL-6- producing T cells was higher in periodontitis than in gingivitis. Moreover, a siginificant positive correlation between the proportion of IL-4-producing cells and the B cell : T cell ratio was noted. These data suggest that gingival T cells are not randomly mobilized from peripheral blood and that local events influence the TCR repertoire at the level of T cell recruitment or T cell expansion and the functional subsets of those T cells are Th2 type. Less

为了解牙周炎患者牙龈组织中T细胞浸润的特点，采用免疫组织化学方法对牙周炎患者牙龈组织中T细胞受体谱和细胞因子谱进行了研究。在完成初始治疗后进行牙周手术时获得牙周炎组织。作为对照的牙龈炎组织也从非牙周炎病变获得。用碱性磷酸酶-抗碱性磷酸酶法对每个标本的连续冰冻切片进行TCR V基因产物（V α 2、V β 5.2 -3、V β 5.3、V β 5.1、V β 6.7、V β 8、V β 12）和细胞因子（IL-2、IFN-γ、IL-4、IL-6）的免疫染色。通过将每个阳性细胞的数量除以CD 3阳性细胞的总数来计算相对百分比。此外，牙龈组织和循环池之间的比较，外周血单个核细胞从两组进行了分析的TCR库通过流式细胞术。免疫组化法检测TCRV基因产物表达， ...更多信息 发炎牙龈表现出个体差异，但出现一致的模式。Vbeta 5亚家族和Vbeta6.7是牙龈中常用的库，而Valpha 2和Vbeta 8亚家族在大多数情况下表达不足。牙龈组织中TCR V基因产物的表达与自体外周血相比存在偏倚。10例牙周炎患者中有3例显示与自体血相比，1或2个显著过高的剧目。在这3例受试者中，2例Vbeta6.7过表达，1例Vbeta5.2-3、Vbeta 8和Vbeta 12过表达。在细胞因子方面，IL-4和IL-6产生T细胞的比例在牙周炎中高于牙龈炎。此外，IL-4产生细胞的比例与B细胞：T细胞比率之间存在显著正相关。这些数据表明，牙龈T细胞不是随机动员外周血和局部事件的影响TCR库的水平上的T细胞募集或T细胞扩增和功能的那些T细胞的亚群是Th 2型。少

项目成果

Kazuhisa Yamazaki: "IL-4 and IL-6-producing cells in human periodontal disease tissue" Journal of Oral Pathology and Medicine. 23. 347-353 (1994)

Kazuhisa Yamazaki：“人类牙周病组织中产生 IL-4 和 IL-6 的细胞”《口腔病理学与医学杂志》。

Nakajima, T., Yamazaki, K., Hara, K: "Biase T cell receptor V gene usage in tissues with periodontal disease" J.Periodont.Res. (in press.). (1995)

Nakajima, T.、Yamazaki, K.、Hara, K：“偏向 T 细胞受体 V 基因在牙周病组织中的使用”J.Periodont.Res。

Toshihiko Aoyagi: "IL-4 and IL-6-producing memory T-cells in peripheral blood and gingival tissues in periodontitis patients with hight serum antibody titers to Perphyromonas gingivalis." Oral Microbiology and Immunology. (印刷中).

Toshihiko Aoyagi：“牙周炎患者的外周血和牙龈组织中产生 IL-4 和 IL-6 的记忆 T 细胞，具有高血清牙龈卟啉单胞菌抗体滴度。”

Takako Nakajima: "Biased T cell receptor Vgene usage in tissues with periodontal disease" Journal of Periodontal Research. (印刷中).

Takako Nakajima：“牙周病组织中偏向 T 细胞受体 V 基因的使用”《牙周研究杂志》（正在出版）。

Kazuhisa Yamazaki: "Immunohistological analysis of T-cell functional subsets in chronic inflammatory periodontal disease." Clinical and Experimental Immunology. 99. 384 (1995)

Kazuhisa Yamazaki：“慢性炎症性牙周病 T 细胞功能亚群的免疫组织学分析。”