Establishment of Diagnostic Methods of Periodontal Disease Basing on the Alteration of Immunocompetent Cells

基于免疫活性细胞变化的牙周病诊断方法的建立

• 批准号: 03807127
• 负责人: YAMAZAKI Kazuhisa
• 金额: $ 1.22万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807127/
• 关键词: Periodontitis; Gingivitis; Memory T lymphocyte; Activated B lymphocyte; IL-4; LFA-1; ICAM-1; Immunohistochemistry; メモリ-T細胞

Recent studies showed that CD45RO+ memory T-cells play important roles in the immunoglobulin production of B-cells by producing various B-cell activating cytokines and cell-cell contact due to enhanced expression of adhesion molecules. This study was designed to investigate frequency and distribution of memory T-cells and activated B-cells in cryostat sections of adult periodontitis and gingivitis lesions by immunohistochemical double staining. We used the same technique to examine T-B interactions in situ by staining: T-cells producing IL-4 which enhances CD23 expression on B-cells, LFA-1 on T-cells, and ICAM-1 on B-cells. Nineteen gingival biopsies were obtained at the time of periodontal surgery following the completion of initial preparation and 5 gingivitis biopsies were obtained from premolar sites requiring extraction for the orthodontic treatment and third molar sites. Serial 6 um-thick cryostat sections were obtained from the central part of each biopsy. Double staining by avi … More din-biotin- immunoperoxidase and avidin-biotin-alkaline phosphatase or alkaline phosphatase anti-alkaline phosphatase techniques were used to identify CD4+CD45RO+ memory T-cells, IL-4+CD45RO+ cells, LFA-1+CD45RO+ cells, ICAM-1+CD19+ B-cells and CD23+ or CD25+,CD19+ activated B-cells. The mean percentage of CD45RO+ memory cells among CD4+ cells was consistently high (65.9% in the crevicular one-third, 61.2% in the middle one-third, 62.5% in the oral one-third), whereas the mean percentage of CD45RA+ naive cells among CD4+ cells was low (17.1% in the crevicular one-third, 14.8% in the middle one-third and 12.4% in the oral one-third). In gingivitis biopsies, T-cells were the major cell type and most of CD4+ cells were CD45RO+. IL-4 producing cells were observed as clusters and/or single cells with various staining intensity in 14 out of 19 periodontal tissues, however, they were much lower in number compared with CD45RO+ cells. On the other hand, the percentage of CD23+ and CD25+, CD19+ B-cells displayed pronounced individual variations (0-100% for CD23, 0-36.2% for CD25) even though the clinical status was similar. Some CD19+ B-cells with enhanced expression of ICAM-1 were identified. The above findings suggest that: 1)early activation events of T-cells and B-cells may take place possibly in the regional lymphnodes before infiltrating into the periodontitis lesion; 2)B-cells infiltrating into the periodontitis lesion displayed features attributable to several different stages of differentiation pathway into plasma cells. Less

最近的研究表明，CD 45 RO+记忆T细胞通过产生各种B细胞活化细胞因子和细胞-细胞接触（由于粘附分子的表达增强）在B细胞的免疫球蛋白产生中起重要作用。本研究采用免疫组织化学双染法检测了成年人牙周炎和牙龈炎组织中记忆性T细胞和活化性B细胞的频率和分布。我们使用相同的技术通过染色原位检查T-B相互作用：T细胞产生IL-4，其增强B细胞上的CD 23表达，T细胞上的LFA-1和B细胞上的ICAM-1。在完成初步准备后进行牙周手术时获得19份牙龈活检，并从需要拔牙的前磨牙部位和第三磨牙部位获得5份牙龈炎活检。从每个活检的中心部分获得连续的6 μ m厚的低温恒温切片。avi双重染色 ...更多信息 采用免疫组织化学技术和抗生物素蛋白-生物素-碱性磷酸酶或碱性磷酸酶抗碱性磷酸酶技术鉴定CD 4 + CD 45 RO+记忆T细胞、IL-4+ CD 45 RO+细胞、LFA-1+ CD 45 RO+细胞、ICAM-1+ CD 19 + B细胞和CD 23+或CD 25+、CD 19+活化B细胞。CD 45 RO+记忆细胞在CD 4+细胞中的平均百分比一直很高CD 4+细胞中CD 45 RA+幼稚细胞的平均百分比较低（17.1%在沟1/3，14.8%在中1/3，12.4%在口1/3）。在牙龈炎活检中，T细胞是主要的细胞类型，并且大多数CD 4+细胞为CD 45 RO+。在19例牙周组织中，有14例IL-4分泌细胞呈不同强度的簇状或单个细胞，但其数量远低于CD 45 RO+细胞。另一方面，尽管临床状态相似，但CD 23+和CD 25+、CD 19 + B细胞的百分比显示出明显的个体差异（CD 23为0-100%，CD 25为0-36.2%）。鉴定了一些ICAM-1表达增强的CD 19 + B细胞。上述结果提示：1）T细胞和B细胞的早期活化事件可能发生在局部淋巴结中，然后才浸润到牙周炎病灶中; 2）浸润到牙周炎病灶中的B细胞具有向浆细胞分化的多个不同阶段的特征。少