Modeling of Pigment Glycoside Production and Its Regulation by Genetic Transformation in Plant Cell Culture.

植物细胞培养中色素糖苷生产的建模及其遗传转化的调节。

基本信息

  • 批准号:
    08455379
  • 负责人:
  • 金额:
    $ 4.42万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

The purpose of the study is to construct a novel method to determine the distribution of pigment accumulation among each cell in plant cell culture. As the results of the quantification of this distribution, a model describing pigment accumulation and its variation through culture period was made for the selection of cell lines and the genetic transformation in pigment production pathway.1) Quantification of an anthocyanin production profile in strawberry cell culture :A new method for the quantitative determination of pigment accumulation profiles in each cell using image analysis was constructed and verified as a reproducible and practical method.2) Genetic transformation of anthocyanin formation pathway for the enhancement of the pigment productivity in strawberry cultured cells :It was verified that the addition of phenylalanine as a precursor in the culture medium enhanced the anthocyanin productivity in strawberry cells. As the uptake rate of the precursor into the cells suggests that one of the rate-limiting enzyme in an anthocyanin production pathway is a CHS,the genetic transformation by the introduction of a CHS gene into the strawberry cells was tried to enhance the productivity. For the genetic analysis of a mutant in anthocyanin-producing cell lines, a continuous culture method was applied for the stabilization of gene expression in anthocyanin production pathway. Cloning of specific gene expressed in the mutant was investigated using a proposed method with a continuous culture and cDNA subtraction.
本研究旨在建立一种测定植物细胞培养过程中色素积累分布的新方法。根据这种分布的量化结果,建立了色素积累及其随培养周期变化的模型,用于细胞系的选择和色素产生途径的遗传转化。1)草莓细胞培养中花青素生产谱的定量分析:构建了一种利用图像分析定量测定每个细胞中色素积累谱的新方法,并验证了该方法的可重复性和实用性。2)通过遗传转化花青素形成途径提高草莓培养细胞色素产量:证实在培养基中添加苯丙氨酸作为前体,可以提高草莓细胞的花青素产量。由于前体进入细胞的摄取速率表明,花青素生产途径中的限速酶之一是CHS,因此试图通过将CHS基因引入草莓细胞的遗传转化来提高其产量。为了对花青素产生细胞系突变体进行遗传分析,采用连续培养的方法稳定了花青素产生途径的基因表达。采用连续培养法和cDNA减法对突变体中表达的特定基因进行了克隆研究。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
W.Zhang: "Effect of Temperature and its Shift of Growth and Anthocyanin Production in Suspension Cultures of Strawberry Cells" Plant Sci.127. 207-214 (1997)
W.Zhang:“草莓细胞悬浮培养物中温度及其生长和花青素产量变化的影响”《植物科学》127。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K.Miyanaga: "Growth and Pigment Accumulation of Cultured Strawberry Cells in the Dark" Chem.Eng.Symp.Ser.57. 157-160 (1997)
K.Miyanaga:“培养草莓细胞在黑暗中的生长和色素积累”Chem.Eng.Symp.Ser.57。
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
W.Zhang, M.Seki and S.Furusaki: "Effect of Temperature and its Shift on Growth and Anthocyanin Production in Suspension Cultures of Strawberry Cells" Plant Sci.127. 207-214 (1997)
W.Zhang、M.Seki 和 S.Furusaki:“温度及其变化对草莓细胞悬浮培养物生长和花青素生产的影响”《植物科学》127。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K.Miyanaga, M.Nakamura, M.Seki and S.Furusaki: "Growth and Pigment Accumulation of Cultured Strawberry Cells in the Dark" Chem.Eng.Symp.Ser.57. 153-156 (1997)
K.Miyanaga、M.Nakamura、M.Seki 和 S.Furusaki:“培养草莓细胞在黑暗中的生长和色素积累”Chem.Eng.Symp.Ser.57。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
W.Zhang, M.Seki and S.Furusaki: "Regulation of Anthocyanin Synthesis in Suspension Cultures of Strawberry Cells by pH" Biotechnol.Lett.19 (11). 1057-1061 (1997)
W.Zhang、M.Seki 和 S.Furusaki:“pH 值对草莓细胞悬浮培养物中花青素合成的调节”Biotechnol.Lett.19 (11)。
  • DOI:
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  • 影响因子:
    0
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FURUSAKI Shintaro其他文献

FURUSAKI Shintaro的其他文献

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{{ truncateString('FURUSAKI Shintaro', 18)}}的其他基金

Development of efficient production of antitumor agents originated from plant and their application to antitumor therapy using hybrid liposomes
植物源抗肿瘤药物高效生产的开发及其在混合脂质体抗肿瘤治疗中的应用
  • 批准号:
    14350440
  • 财政年份:
    2002
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of Artificial Enzymes by Surface Molecular Imprinting
通过表面分子印迹开发人工酶
  • 批准号:
    12450335
  • 财政年份:
    2000
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of Surfactant Modified Enzymes That Show High Activity in Organic Media.
开发在有机培养基中表现出高活性的表面活性剂修饰酶。
  • 批准号:
    10555284
  • 财政年份:
    1998
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Evaluation of Effect of Hydrodynamic Shear Stress on Cultured Plant Cells
水动力剪切应力对培养植物细胞影响的评价
  • 批准号:
    07555255
  • 财政年份:
    1995
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Synthesis and Application of Intelligent interface System
智能接口系统的综合与应用
  • 批准号:
    07305036
  • 财政年份:
    1995
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of Production Process for Anti-Cancer Drug Taxol with Immobilized Plant Cell Culture.
固定化植物细胞培养抗癌药物紫杉醇生产工艺的开发。
  • 批准号:
    06453106
  • 财政年份:
    1994
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Research on Plant Cell Culture with Ultrasonic Permeabilization for the Release of Intracellular Product
植物细胞超声透化释放细胞内产物的研究
  • 批准号:
    04453118
  • 财政年份:
    1992
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Development of Functional Porous Membrane Prepared by Radiation-Induced
辐射诱导制备功能性多孔膜的研制
  • 批准号:
    02555187
  • 财政年份:
    1990
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Research on the Effect of Light Irradiation on Metabolite Production using Cultured Plant Cell
利用培养植物细胞研究光照射对代谢产物产生的影响
  • 批准号:
    02453115
  • 财政年份:
    1990
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Study on Production of Useful Chemicals using Immobilized Plant Cells
利用固定化植物细胞生产有用化学品的研究
  • 批准号:
    63470102
  • 财政年份:
    1988
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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