权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of Surfactant Modified Enzymes That Show High Activity in Organic Media.

开发在有机培养基中表现出高活性的表面活性剂修饰酶。

基本信息

批准号：
10555284
负责人：
FURUSAKI Shintaro
金额：
$ 8.19万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

The utilization of enzymes in nonaqueous media has been probed to be one of the simplest but the most promising techniques for the enantioselective conversion of various hydrophobic acids and alcohol in the last decade. The high enantioselectivity of enzymes is an attractive feature, in particular, for producing optically active compounds. In our recent investigation, a surfactant-enzyme complex was found to be catalytically active in nonaqueous media. The preparation method of surfactant-enzyme complexes utilizing W/O emulsions is very simple and does not require complicating techniques.Proteases modified by this preparation method showed high activity in anhydrous organic solvents such as isooctane and toluene. In the present study, a peptide synthesis catalyzed by surfactant-protease complex is conducted in anhydrous organic solvents. The effects of origin of proteases, addition of water, organic solvents, cosolvents were discusses ad well as that of the kind of substrates on the enzymatic activity. The surfactant-protease complexes were thermostable at high temperature, and showed a high storage stability.The surfactant-lipase complexes were also thermostable at high temperature compared to the native lipase. Furthermore, the lipase complex showed a high storage stability. This modification method will enable us to utilize a lot of enzymes as new biocatalysts which had not shown enzymatic activity in organic media.

非水相酶催化是近十年来发展起来的最简单、最有前途的疏水性酸和醇的不对称选择性转化技术之一。酶的高对映体选择性是一个有吸引力的特征，特别是对于生产光学活性化合物。在我们最近的研究中，发现表面活性剂-酶复合物在非水介质中具有催化活性。利用W/O型乳状液制备表面活性剂-酶复合物的方法非常简单，不需要复杂的工艺，用这种方法修饰的蛋白酶在异辛烷和甲苯等无水有机溶剂中表现出很高的活性。在本研究中，在无水有机溶剂中进行了表面活性剂-蛋白酶复合物催化的肽合成。讨论了蛋白酶的来源、水的加入量、有机溶剂、助溶剂以及底物种类对酶活力的影响。该复合物在高温下具有较好的热稳定性，并具有较高的贮存稳定性，与天然脂肪酶相比，该复合物在高温下也具有较好的热稳定性。此外，脂肪酶复合物显示出高的储存稳定性。这种修饰方法将使我们能够利用许多在有机介质中没有表现出酶活性的酶作为新的生物催化剂。