Research on the Effect of Light Irradiation on Metabolite Production using Cultured Plant Cell

1)光照对咖啡细胞次生代谢产物产生的影响为植物细胞培养的工业化应用，采用化学辐射法研究了光照对小粒咖啡细胞生产咖啡因的影响。咖啡因的生产阿拉比卡咖啡细胞贪婪的光增加。在分批培养中，大约需要六天的照射时间来增加咖啡因的产量。在0.11 J/L/S的低光照率下，咖啡因产量显著增加。同时，细胞生长受到抑制，通过增加照射速率。结果表明，有一个光照射速率，优化生产。由于长时间的连续光照极大地抑制了细胞的生长，因此将培养分为两个阶段：光照下的生产期和黑暗下的生长期。2）单细胞吸光率的测定我们建立了一种新的利用化学光度法定量测定细胞悬浮液中光透射与反射比的方法，并测定了单细胞的吸光率，为光生物反应器的设计提供了有用的信息。生物反应器我们提出了带吸附柱的培养系统来去除产物，并取得了良好的效果。在鼓泡塔反应器中使用植物细胞的固定化细胞系统获得了更高的生产率和细胞生长。

1) Effect of light irradiation on the secondary-metabolite production of coffee plant cellsThe effect of light on caffeine production by Coffea arabica cells was investigated using chemical actinometry for the purpose of industrial application of plant cell cultures. Caffeine production by the Coffea arabica cells was greedy increased by light. About a six-day irradiation period was required to increase the caffeine production in a batch culture. Caffeine production significantly increased at a low light irradiation rate of 0.11 J/L/S. Meanwhile, cell growth was suppressed by increasing the irradiation rate. It was shown there was a light irradiation rate that optimized production. Since longterm continuous light greatly suppressed cell growth, the culture was divided into two stages : the production period in the light and the growth period in the dark. The high production and good growth were maintained for 65 days.2) Estimation of light adsorption rate by a cell.We developed a novel method for quantitative determination of the ratio of light transmission to reflection in cell suspension using chemical actinometry, and estimated light adsorption rate by a cell, which presents useful information for design strategy of photobioreactor.3) Construction of photo-bioreactorsWe proposed the culture system with adsorption column for the product removal and presented good results. Immobilized cell system using the plant cell in a bubble column reactor achieved higher productivity and cell growth.