Production of iron-deficiency tolerant transgenic tobacco introduced with ferric reductase

引入铁还原酶的耐缺铁转基因烟草的生产

• 批准号: 06453161
• 负责人: MORI Satoshi
• 金额: $ 2.82万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453161/
• 关键词: Saccharomyces cereviceae; FRE1; CaMV35S; transgenic tobacco; BPDS; Fe deficiency; PCR; FREI; トランスジエニツク・タバコ; Agrobacterium; Fe^<3+>-reductase; ylast; Nicotiana tabacum; pBI121; Fe-deficiencuy

FRE1 gene was introduced into tobacco. However, the strains which showed significant reductase activity than the control could not be obtained. The length of the mRNA of the introduced gene was observed shorter than the FRE1. This means its transcription starting point was the downstream than the control or the termination of the transcription was upper stream than the control. By introducing several types of point mutagenesis into FRE1 gene, the transgenic plants are again under construction.

将FRE1基因导入烟草。但未能获得还原酶活性显著高于对照的菌株。观察到导入基因的mRNA的长度短于FRE1。这意味着其转录起始点位于对照的下游或转录终止点位于对照的上游。通过对FRE1基因进行点突变，再次构建了转基因植株。

项目成果

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