Searching the Fe^3+-chelate stable under high pH conditions

3种油橄榄（Olea europaea var.欧罗巴湾使命，和var.在人工气候室中，在30 °C（14小时-光照）/25°C（10小时-黑暗）的条件下，在1 L盆的碱性土壤中培养15 cm的Neva）。生长2年后，从根部5cm处切下嫩枝，收集木质部分泌物，置于3 mL Eppendorf试管中，用铝箔覆盖，培养48小时。用70%乙醇提取棉花，并蒸发乙醇。用MiliQ-水溶解各残留物，并通过Tof-Mass进行代谢物组分析。在所有油橄榄属植物中检测到的化学成分为6087种。结果表明，油橄榄木质部汁液中合成的化学物质的数量为1,527种，碱性土壤比中性土壤更为专一。木卫二，978年在瓦尔。使命和4 173在瓦尔涅瓦河。供试的3个盐碱土品种共有的化学物质为469种。在今后的研究中，我将选择黄嘌呤和异羟肟酸作为Fe^3+螯合剂的候选化合物。例如，出乎意料地，检测到烟酰胺，尽管量非常低。

Three Olive species (Olea europaea var. Europa, var. Mission, and var. Neva ) of 15 cm were cultivated in alkaline soil of 1 L pots in the phytotron under the conditions at30°C (14 hr- light)/25°C (10 hr-dark). After two years’ growth the shoots were cut at 5cm hight from the roots and xylem exudates were harvested to the cotton wool in the3mL Eppendorf tubes covered with aluminum foil for 48 hrs. The cottons were extracted with 70% ethanol and the ethanol was evaporated. Each residue was solubilized with MiliQ-water and was supplied for the Metabolome-analysis by Tof-Mass. The detected chemical compounds in all Olea species were 6087. The Number of the chemicals which have been synthesized and contained in xylem sap of Olea rather specific to alkaline soil than neutral soil was1,527 in var. Europa, 978 in var. Mission and 4,173 in var Neva. The number of the common chemicals among three varieties specific to alkaline soil was 469. I will select the compounds of chatecols and /or hydroxamate as the candidates for the chelating agents of Fe^3+ in future. For example, unexpectedly, nicotianamine was detected although the amount was very low.