Development of PET for the analysis of plan response to environmental stresses

开发 PET 用于分析环境压力的计划响应

• 批准号: 12356003
• 负责人: MORI Satoshi
• 金额: $ 25.38万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12356003/
• 关键词: PETIS; ^<52>Fe-mugineic acid; ^<13>NO_3; ^<11>C-methionine; ^<11>C-IAA; H_2^<15>O; ^<52>Mn; ^<62>Zn; ^<13>NO_<3^->; メチル水銀; ABA; 52Fe; 52Mn; 62Zn; イネ; ^<13>NH_4^+; ^<52>Fe; ^<13>N-NH_4^+; ^<13>N-NO_3^-

At Hamamatsu Photnics KK we used 13NO3-, 13NH4+, 11C-methionine, 11C-IAA and H215O, and at Takasaki Institute of Japan Atomic Energy Research Institute, we used ^<52>Fe-mugineic acid, ^<52>Mn, ^<62>Zn for the analysis of absorption, translocation, and retranslocation of those compounds in plants. Those dynamic images were visualized as movies. In case of rice velocity from the roots to the discrimination center (DC) and in the leaf was calculated. In every mineral elements, when the plants were treated with elemental deficiency, demands for those elements were increased, the time for translocation from the roots to DC were increased several folds. These results suggested that the specific transporter for each element were induced by the elemental deficiency in the roots in the transcriptional level. In the graminaceous plants 'ys1' gene was first cloned by Walker et al (2002) in corn. In the rice geneome database we found 16 ys1-like genes (: ysL), and among them several genes were expressed in the roots or shoots by the treatment of Fe-denciency by northern analysis. We also cloned Fe_<2+>-transporter genes, OsIRT1 and OsIRT2. Both genes were induced by Fe-deficiency in rice roots. Expression of those iron-related genes in rice tissues are underway in the mstochemical method using transgenic rice introduced with ,ysL promotor gus and OsIRT1 promotor gus. However, Zn_<2+>- and Mn_- transporter have never been cloned in graminaceous plants. H_2^<15>O was first monitored in the bean plants. This was shown as a movie and is displayed in our home-page, ・http://park.itc.u-tokyo.ac.jp/pnip/

在Hamamatsu photonics KK，我们使用了13NO3-， 13NH4+， 11c -蛋氨酸，11C-IAA和H215O，在日本原子能研究所的高崎研究所，我们使用了^<52> fe - mugineacid, ^<52>Mn, ^<62>Zn来分析这些化合物在植物中的吸收、易位和再易位。那些动态的图像被视觉化为电影。在水稻的情况下，计算了从根部到识别中心（DC）和叶片的速度。在所有矿质元素中，当植物元素缺乏时，对这些元素的需要量增加，从根部转运到DC的时间增加了数倍。这些结果表明，在转录水平上，每个元素的特异性转运体是由根中元素缺乏诱导的。在禾本科植物中，“ys1”基因最早由Walker等人（2002）在玉米中克隆。在水稻基因组数据库中，我们发现16个ys1样基因（:ysL），其中有几个基因在铁胁迫处理下在根或芽中表达。我们还克隆了Fe_<2+>-转运蛋白基因OsIRT1和OsIRT2。这两个基因都是由水稻根系缺铁诱导的。这些铁相关基因在水稻组织中的表达是通过引入ysL启动子gus和OsIRT1启动子gus的转基因水稻进行的。然而，在禾本科植物中尚未克隆到Zn_<2+>-和Mn_-转运体。在豆科植物中首次监测到H_2^<15>。这是一部电影，并显示在我们的主页，·http://park.itc.u-tokyo.ac.jp/pnip/

项目成果

Kiyomiya S. et al.: "Light activates H_2^<15>O flow in rice-detailed monitoring using a positron-emitting tracer imaging system (PETIS)"Physiologia Plantarum. 113. 359-367 (2001)

Kiyomiya S.等人：“使用正电子发射示踪成像系统(PETIS)在水稻详细监测中光激活H_2^ 15 O流”Physiologia Plantarum。

Mori S et al.…Tsuji S: "Visualization of ^<15>O-water flow in tomato and rice in the light and dark using a positron-emitting tracer imaging system (PETIS)"Soil Sci Plant Nutr. 46. 975-979

Mori S 等人…Tsuji S：“使用正电子发射示踪成像系统 (PETIS) 可视化番茄和水稻中 ^<15>O 水流的光和暗”土壤科学植物营养 46. 975-第979章

Kiyomiya S. et al.: "Real time visualization of ^<13>N translocation in rice under different environmental conditions using positron emitting tracer imaging system"Plant Physiology. 125. 1743-1753 (2001)

Kiyomiya S.等人：“使用正电子发射示踪成像系统在不同环境条件下对水稻中^ 13 N易位进行实时可视化”植物生理学。

Kiyomiya S. et al.: "light activate ^<15>O-waterflow in rice -detailed monitoring using a position emitting traces imaging system(PETIS)"Physiologia Plantarum. (in press). (2001)

Kiyomiya S.等人：“光激活水稻中的^ 15 O-水流-使用位置发射痕迹成像系统(PETIS)进行详细监测”Physiologia Plantarum。

Bughio N et al.: "Cloning an iron regulated metal transporter from rice"J Exp Bot. 53. 1677-1682 (2002)

Bughio N 等人：“从水稻中克隆铁调节的金属转运蛋白”J Exp Bot。