Regulation of Iron-deficincy Tolerance in Plants

植物耐缺铁性的调节

• 批准号: 13306007
• 负责人: MORI Satoshi
• 金额: $ 11.81万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13306007/
• 关键词: alkaline soils; tolerance to Fe deficiency; nicotianamine aminotransferase(Hvnas1); micotianamine aminotransferase(HvnaatA); Ids2; promoter gus; Iron deficiency responsive elements(IDEs); Ids3; 鉄欠乏; シスエレメント(IRE); トランス因子(IRBP); ムギネ酸; コウボ; one-

1. We have cloned all genes on the mugineic acid synthesis in the past two decades. Among them Ids2 (Iron deficiency specific clone no.2) was used for the analysis of iron-deficiency regulatory elements (IDEs) in the upstream region of the ORF. Deletion series of 2 kb Ids2 promoter were fused with gus gene, those genes were introduced into tobacco and GUS activity were analyzed under Fe deficiency in those transgenic tobacco. Two cis elements were identified as necessary parts for induction by Fe deficiency. Minimum sequence of IDE1 was determined as 19 bases by sequential introduction of 3 base mutation into the focused 40 bases, making transgenic tobacco and GUS activity was analyzed for each transgenic plants under Fe deficiency. The minimum sequence of the other IDE2 is under the way.2. We introduced 'Hvnas1-HvnaatA' genes into rice, T2 seeds which had high potential to be tolerant in calcareous soils were selected. Those transgenic rice are now under preparation for field experiment for the governmental permittion.3. We synthesized refrel gene by modification of yeast Fre1 (ferric reductase ) gene by site-directed mutagenesis, and introduced the refre1 genes into rice. T1 plant is now under screening in the pot experiments in calcareous soils.

1. 在过去的二十年里，我们已经克隆了所有的氨基酸合成基因。其中Ids2（缺铁特异性克隆）；2)用于分析ORF上游区域的缺铁调节元件（IDEs）。将缺失的2kb Ids2启动子序列与gus基因融合，将其引入烟草中，分析了缺铁条件下转基因烟草的gus活性。鉴定出两种顺式元素是诱导缺铁的必要成分。通过将3个碱基突变序列依次导入到集中的40个碱基中，确定了IDE1的最小序列为19个碱基，制作了转基因烟草，并分析了缺铁条件下各转基因植株的GUS活性。另一个IDE2的最小序列正在进行中。将“Hvnas1-HvnaatA”基因引入水稻中，筛选出具有较高耐钙质土壤潜力的T2种子。这些转基因水稻正在准备进行田间试验，以获得政府的许可。通过位点诱变对酵母铁还原酶（Fre1）基因进行修饰，合成了refrel基因，并将其引入水稻中。T1植株目前正在钙质土壤盆栽试验中进行筛选。

项目成果

Kiyomiya S. et al.: "Real time visualization of ^<13>N translocation in rice under different environmental conditions using positron emitting tracer imaging system"Plant Physiology. 125. 1743-1753 (2001)

Kiyomiya S.等人：“使用正电子发射示踪成像系统在不同环境条件下对水稻中^ 13 N易位进行实时可视化”植物生理学。

Bughio N et al・・・Mori S: "Cloning an iron regulated metal transporter from rice"J Experimental Botany. 53. 1677-1682 (2002)

Bughio N 等人...Mori S：“从水稻中克隆铁调节的金属转运蛋白”J Experimental Botany 53. 1677-1682 (2002)。

Yamaguchi et al.: "IDI7, a new iron-regulated ABC transporter from barley roots, localizes to the tonoplast"J Exp Bot. 53. 727-735 (2002)

Yamaguchi 等人：“IDI7，一种来自大麦根的新型铁调节 ABC 转运蛋白，定位于液泡膜”J Exp Bot。

Yoshihara T et al.: "Analysis of some divalent metal contents in tobacco expressing the exogenous soybean ferritin gene"J Plant Nutr. (in press). (2003)

Yoshihara T等：“表达外源大豆铁蛋白基因的烟草中一些二价金属含量的分析”J Plant Nutr。

Yoshihara et al Mori S: "Analysis of some divalent metal contents in tobacco expressing the exogenous soybean ferritin gene"J Plant Nutrition. in press. (2003)

Yoshihara等人Mori S：“表达外源大豆铁蛋白基因的烟草中一些二价金属含量的分析”J Plant Nutrition。