Molecular mechanisms of activin B-induced spermatogenesis
Molecular mechanisms of activin B-induced spermatogenesis
批准号:
06454022
负责人:
NAGAHAMA Yoshitaka
金额:
$4.35万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1995
中文摘要
本研究旨在了解促性腺激素诱导鳗鱼精子发生的分子机制。将含有激活素BETAB亚单位基因的表达载体导入哺乳动物CHO细胞后,可分泌出一个22 kDa的蛋白。纯化的重组蛋白(鳗鱼激活素B)具有红系分化因子活性。在培养液中加入鳗鱼激活素B,在15天内以与11-酮睾酮相同的方式诱导精原细胞增殖,产生晚期B型精原细胞。综上所述,支持细胞在促性腺激素和11-酮睾酮的作用下产生的激活素B在鱼类睾丸的生精调控中起着重要作用。我们还从鳗鱼睾丸的cDNA文库中克隆了编码细胞激活素I和II受体的cDNAs。Northern印迹分析表明,在注射促性腺激素之前,激活素I和II受体的mRNA转录产物在鳗鱼的睾丸中表达。原位杂交显示这些受体定位于生殖细胞(精原细胞和精母细胞)和支持细胞,这为激活素B可能直接作用于生殖细胞提供了证据。已从鳗鱼精巢中克隆了几个编码G1/S细胞周期蛋白的基因(A1、A2、D1、D2、E1、E2)。在激素诱导生精之前,这些G1/S细胞周期蛋白转录本均不存在于鳗鱼的睾丸中。注射促性腺激素后,这些细胞周期蛋白在睾丸中的表达顺序如下。:E1(G1/S)*A2,B1,B2*A1(精母细胞)。
英文摘要
The purpose of this study is to understand the molecular mechanisms of gonadotropin-induced spermatogenesis in the Japanese eel (Anguilla japonica). With introduction of the expression vector containing activin betaB subunit cDNA into mammalian CHO cells, these cells secreted a 22kDa protein into the medium. Purified recombinant protein (eel activin B) had erythroid differentiation factor activity. Addition of eel activin B to the culture medium induced proliferation of spermatogonia, producing late type B spermatogonia, within 15 days in the same manner of 11-ketotestosterone. Taken together, it is concluded that activin B produced by Sertoli cells under the influence of gonadotropin and 11-ketotestosterone plays an important role in the regulation of spermatogenesis in fish testes. We have also cloned cDNAs encoding ell activin type I and II receptors from an eel testis cDNA library. Northern blot analysis reveals that mRNA transcripts of activin type I and II receptors are expressed in testes of eels prior to gonadotropin injection. In situ hybridization shows that these receptors are localized in germ cells (spermatogonia and spermatocytes) and Sertoli cells, providing evidence that activin B may act directly on the germ cells. Several cDNAs encoding G1/S cyclins (A1, A2, D1, D2, E1, E2) have been cloned from eel testes. None of these G1/S cyclin transcripts were present in testes of eels before hormonal induction of spermatogenesis. The following sequence of expression of these cyclins was observed in testes after gonadotropin injection. : E1 (G1/S) *A2, B1, B2*A1 (spermatocytes).
期刊论文(44)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Miura, T.et al.: "Human recombinant activin induces proliferation of spermatogonia in vitro in the Japanese eel,Anguilla Japonica" Fisheries Research. 61. 434-437 (1995)
Miura, T.等人:“人类重组激活素在日本鳗鲡体外诱导精原细胞增殖”渔业研究。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Nagahama, Y., Miura, T.and Kobayashi, T.: "The onset of spermatogenesis in fish." Chiba Foundation Symposium on Germline Development. 255-270 (1994)
Nagahama, Y.、Miura, T. 和 Kobayashi, T.:“鱼类精子发生的开始。”
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Nagahama,Y.: "Endocrine regulation of gametogenesis in fish" International Journal of Developmental Biology. 38. 217-229 (1994)
Nagahama,Y.:“鱼类配子发生的内分泌调节”国际发育生物学杂志。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
岡田 益吉, 長濱 嘉孝(編): "生殖細胞-形態から分子へ-" 共立出版, 223 (1996)
Masukichi Okada、Yoshitaka Nagahama(编):“生殖细胞 - 从形态到分子”Kyoritsu Shuppan,223(1996)
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Miura,T.et al.: "Acquisition of potential for sperm motility in Japanese eel Auguilla japonica" Fisheries Science. 61. 533-534 (1995)
Miura,T.et al.:“日本鳗鲡精子活力潜力的获取”渔业科学。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
共 22 条
Molecular mechanisms of sexual plasticity in adult medaka gonads
-
批准号:24370028
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.32万
-
财政年份:2012
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Molecular mechanisms of ovarian differentiation induced by sex hormones
-
批准号:20247004
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$31.28万
-
财政年份:2008
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Mechanisms of sex determination and gonadal sex differentiation in fish
-
批准号:16086213
-
项目类别:Grant-in-Aid for Scientific Research on Priority Areas
-
资助金额:$47.87万
-
财政年份:2004
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Molecular Mechanisms of Sex Change in Fish
-
批准号:16370032
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$9.54万
-
财政年份:2004
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Functions and Mechanisms of Action of the Medaka Sex-Determining Gene
-
批准号:14340259
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$9.02万
-
财政年份:2002
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Molecular mechanisms of gamete maturation-inducing hormone synthesis
-
批准号:10440247
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$7.55万
-
财政年份:1998
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Molecular mechanisms of oocyte and sperm maturation-inducing hormone
-
批准号:08454266
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$5.06万
-
财政年份:1996
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Molecular Mechanisms of Germ Cell Formation
-
批准号:07283104
-
项目类别:Grant-in-Aid for Scientific Research on Priority Areas
-
资助金额:$190.34万
-
财政年份:1995
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
Molecular Mechanisms of Sex Determination and Differentiation
-
批准号:06044235
-
项目类别:Grant-in-Aid for international Scientific Research
-
资助金额:$7.74万
-
财政年份:1994
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
General principle involved in the mechanism of oocyte maturation
-
批准号:04044177
-
项目类别:Grant-in-Aid for international Scientific Research
-
资助金额:$4.86万
-
财政年份:1992
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
M/lecular regulation of germ cell formation
-
批准号:02102010
-
项目类别:Grant-in-Aid for Specially Promoted Research
-
资助金额:$112.0万
-
财政年份:1990
-
负责人:NAGAHAMA Yoshitaka
-
依托单位:
海外基金