Molecular mechanisms of activin B-induced spermatogenesis
激活素 B 诱导精子发生的分子机制
基本信息
- 批准号:06454022
- 负责人:
- 金额:$ 4.35万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The purpose of this study is to understand the molecular mechanisms of gonadotropin-induced spermatogenesis in the Japanese eel (Anguilla japonica). With introduction of the expression vector containing activin betaB subunit cDNA into mammalian CHO cells, these cells secreted a 22kDa protein into the medium. Purified recombinant protein (eel activin B) had erythroid differentiation factor activity. Addition of eel activin B to the culture medium induced proliferation of spermatogonia, producing late type B spermatogonia, within 15 days in the same manner of 11-ketotestosterone. Taken together, it is concluded that activin B produced by Sertoli cells under the influence of gonadotropin and 11-ketotestosterone plays an important role in the regulation of spermatogenesis in fish testes. We have also cloned cDNAs encoding ell activin type I and II receptors from an eel testis cDNA library. Northern blot analysis reveals that mRNA transcripts of activin type I and II receptors are expressed in testes of eels prior to gonadotropin injection. In situ hybridization shows that these receptors are localized in germ cells (spermatogonia and spermatocytes) and Sertoli cells, providing evidence that activin B may act directly on the germ cells. Several cDNAs encoding G1/S cyclins (A1, A2, D1, D2, E1, E2) have been cloned from eel testes. None of these G1/S cyclin transcripts were present in testes of eels before hormonal induction of spermatogenesis. The following sequence of expression of these cyclins was observed in testes after gonadotropin injection. : E1 (G1/S) *A2, B1, B2*A1 (spermatocytes).
本研究的目的是了解促性腺激素诱导日本鳗鲡精子发生的分子机制。将含有激活素β B亚基cDNA的表达载体导入哺乳动物CHO细胞后,这些细胞分泌22 kDa的蛋白到培养基中。纯化的重组蛋白(鳗鱼激活素B)具有红系分化因子活性。添加鳗鱼激活素B到培养基中诱导精原细胞增殖,产生晚期B型精原细胞,在15天内以相同的方式11-酮睾酮。综上所述,支持细胞在促性腺激素和11-酮睾酮的影响下产生的激活素B在鱼类睾丸精子发生的调节中起着重要的作用。我们还从鳗鱼睾丸cDNA文库中克隆了编码细胞激活素I型和II型受体的cDNA。北方印迹分析表明,激活素I型和II型受体的mRNA转录本在注射促性腺激素之前的鳗鱼睾丸中表达。原位杂交显示这些受体定位于生殖细胞(精原细胞和精母细胞)和支持细胞,为激活素B可能直接作用于生殖细胞提供了证据。从鳗鱼睾丸中克隆了几个编码G1/S周期蛋白(A1、A2、D1、D2、E1、E2)的cDNA。这些G1/S细胞周期蛋白转录本都不存在于激素诱导精子发生前的鳗鱼睾丸。在注射促性腺激素后的睾丸中观察到这些细胞周期蛋白的表达顺序如下。:E1(G1/S)*A2,B1,B2*A1(精母细胞)。
项目成果
期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Miura, T.et al.: "Human recombinant activin induces proliferation of spermatogonia in vitro in the Japanese eel,Anguilla Japonica" Fisheries Research. 61. 434-437 (1995)
Miura, T.等人:“人类重组激活素在日本鳗鲡体外诱导精原细胞增殖”渔业研究。
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- 影响因子:0
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- 通讯作者:
Nagahama, Y., Miura, T.and Kobayashi, T.: "The onset of spermatogenesis in fish." Chiba Foundation Symposium on Germline Development. 255-270 (1994)
Nagahama, Y.、Miura, T. 和 Kobayashi, T.:“鱼类精子发生的开始。”
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- 影响因子:0
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Nagahama,Y.: "Endocrine regulation of gametogenesis in fish" International Journal of Developmental Biology. 38. 217-229 (1994)
Nagahama,Y.:“鱼类配子发生的内分泌调节”国际发育生物学杂志。
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岡田 益吉, 長濱 嘉孝(編): "生殖細胞-形態から分子へ-" 共立出版, 223 (1996)
Masukichi Okada、Yoshitaka Nagahama(编):“生殖细胞 - 从形态到分子”Kyoritsu Shuppan,223(1996)
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- 影响因子:0
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Miura,T.et al.: "Acquisition of potential for sperm motility in Japanese eel Auguilla japonica" Fisheries Science. 61. 533-534 (1995)
Miura,T.et al.:“日本鳗鲡精子活力潜力的获取”渔业科学。
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- 影响因子:0
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NAGAHAMA Yoshitaka其他文献
NAGAHAMA Yoshitaka的其他文献
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{{ truncateString('NAGAHAMA Yoshitaka', 18)}}的其他基金
Molecular mechanisms of sexual plasticity in adult medaka gonads
成年青鳉性腺性可塑性的分子机制
- 批准号:
24370028 - 财政年份:2012
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of ovarian differentiation induced by sex hormones
性激素诱导卵巢分化的分子机制
- 批准号:
20247004 - 财政年份:2008
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Mechanisms of sex determination and gonadal sex differentiation in fish
鱼类性别决定和性腺性别分化的机制
- 批准号:
16086213 - 财政年份:2004
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular Mechanisms of Sex Change in Fish
鱼类性别变化的分子机制
- 批准号:
16370032 - 财政年份:2004
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functions and Mechanisms of Action of the Medaka Sex-Determining Gene
青鳉性别决定基因的功能和作用机制
- 批准号:
14340259 - 财政年份:2002
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of gamete maturation-inducing hormone synthesis
配子成熟诱导激素合成的分子机制
- 批准号:
10440247 - 财政年份:1998
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of oocyte and sperm maturation-inducing hormone
卵母细胞和精子成熟诱导激素的分子机制
- 批准号:
08454266 - 财政年份:1996
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanisms of Germ Cell Formation
生殖细胞形成的分子机制
- 批准号:
07283104 - 财政年份:1995
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular Mechanisms of Sex Determination and Differentiation
性别决定和分化的分子机制
- 批准号:
06044235 - 财政年份:1994
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for international Scientific Research
General principle involved in the mechanism of oocyte maturation
卵母细胞成熟机制的一般原理
- 批准号:
04044177 - 财政年份:1992
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for international Scientific Research
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