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Functions and Mechanisms of Action of the Medaka Sex-Determining Gene

青鳉性别决定基因的功能和作用机制

基本信息

批准号：
14340259
负责人：
NAGAHAMA Yoshitaka
金额：
$ 9.02万
依托单位：
Okazaki National Research Institutes, National Institute for Basic Biology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340259/
关键词：
Fish Sex-determining gene Testis Sertoli cell Sex differentiation Medaka DMY DMRT1 性決定精巣分化 Y染色体脊椎動物特異抗体

项目摘要

We previously identified PG17 as the leading candidate for the medaka sex-determining gene. To definitively identify PG17 as a sex-determining gene, we injected one-cell stage medaka embryos with a genomic DNA fragment carrying PG17. The transgenic embryos were bred until secondary sexual characters were observed. We found that all XX transgenic fish were male, showing that PG17 is sufficient to induce male development. The present data combined with our previous data indicate that PG17 is the sex-determining gene of medaka and named it DMY.DMY mRNA and protein are expressed specifically in the somatic cells (Sertoli cells) surrounding primordial germ cells (PGC) in the early gonadal primordium, before morphological sex differences are apparent. DMRT1, another DM domain gene that is also involved in male development in other vertebrates, is expressed in spermatogonium-supporting cells after testicular differentiation (20-30 days after hatching). These results strongly suggest that DMY regulates PGC proliferation and differentiation sex-specifically during early gonadal differentiation of XY individuals and that DMRT1 regulates spermatogonial differentiation.Because Sry transgenic mice are sterile and because DMY transgenic XX medakas are fertile, i.e., fully functional males, DMY transgenic medakas are the first transgenic vertebrates known to undergo complete sex reversal.

我们以前确定PG17作为青鳉性别决定基因的主要候选者。为了确定PG17作为性别决定基因，我们将携带PG17的基因组DNA片段注入单细胞期青鳉胚胎。将转基因胚胎培育至观察到第二性征。我们发现所有XX转基因鱼都是雄性，表明PG 17足以诱导雄性发育。DMY基因在青鳉早期性腺原基中的原始生殖细胞（PGC）周围的体细胞（Sertoli细胞）中特异表达，但在形态学性别差异不明显的时期，DMY基因的表达量最高。DMRT1是另一个DM结构域基因，也参与其他脊椎动物的雄性发育，在睾丸分化后（孵化后20 - 30天）在精原细胞支持细胞中表达。这些结果强烈地表明，DMY在XY个体的早期性腺分化期间调节PGC增殖和分化性别特异性，并且DMRT 1调节精原细胞分化。DMY转基因青鳉是已知的第一种经历完全性逆转的转基因脊椎动物。

项目成果

期刊论文数量（54）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Positional cloning of the sex-determining region of medaka using a Y congenic strain

使用 Y 同类株对青鳉性别决定区进行位置克隆

DOI：
发表时间：
2003
期刊：
Aquatic Genomics Steps Toward a Great Future
影响因子：
0
作者：
Matsuda;M. et al.
通讯作者：
M. et al.

M.Matsuda et al.: "DMY is a Y-specific DM-domain gene required for male development in the medaka fish"Nature. 417(6888). 559-563 (2002)

M.Matsuda 等人：“DMY 是青鳉鱼雄性发育所需的 Y 特异性 DM 结构域基因”Nature。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes

DOI：
10.1002/dvdy.20158
发表时间：
2004-11-01
期刊：
DEVELOPMENTAL DYNAMICS
影响因子：
2.5
作者：
Kobayashi, T;Matsuda, M;Nagahama, Y
通讯作者：
Nagahama, Y

M.Matsuda, Y.Nagahama et al.: "DMY is a Y-specific DM-domain gene required fro male development in the medaka"Nature. 417. 559-563 (2002)

M.Matsuda、Y.Nagahama 等人：“DMY 是青鳉雄性发育所需的 Y 特异性 DM 结构域基因”。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Expression of Aromatase mRNA and effects of aromatase inhibitor during ovarian development in the medaka, Oryzias latipes

DOI：
10.1002/jez.a.20027
发表时间：
2004-03-01
期刊：
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
影响因子：
2.8
作者：
Suzuki, A;Tanaka, M;Shibata, N
通讯作者：
Shibata, N