Molecular Mechanisms of Sex Determination and Differentiation
性别决定和分化的分子机制
基本信息
- 批准号:06044235
- 负责人:
- 金额:$ 7.74万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for international Scientific Research
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This international collaborative research is to combine a variety of approaches and animal systems to the study of sex determination and sexual differentiation. Sex change in the endemic, Hawaiian wrasse Thalassoma duperrey provides an ideal model system to investigate mechanisms of sex determination and sexual differentiation. We first used immunocytochemical methods to detect gonadal sites of P450 cholesterol side-chain cleavage enzyme (P450scc) in the wrasse at different stages of in the sex change process. P450scc cells were predominantly in the theca layr of normal females. As females underwent sex change, P450scc localization shifted from the theca layr to the interstitium. P450scc cells appeared to increase in number midway through sex change. The gonad's ability to produce estrogens, however, is permanently lost in the earliest stages of sex change. The levels of aromatase mRNA and protein, the enzyme responsible for converting androgens to estrogens, was undetectable in testes … More and transitional gonads. In contrast, protein levels of 11beta-hydroxylase, the enzyme responsible for 11-ketotestosterone production increase towards the end of sex change process.In this collaborative research, we also examined immunocytochemically the initial differentiation and development of steroid-producing cells during gonadal sex differentiation in tilapia Oreochromis niloticus using polyclonal antibodies against four steroid-metabolizing enzymes (P450scc, 3beta-HSD,P450c17, P450arom). Immunoreaction for P450scc, 3beta-HSD,P450c17, and P450arom became evident for the first time in gonads of genetically females examined at several days before morphological sex differentiation. In contrast, positive immunostaining for all four steroidogenic enzymes was not easily identified in the testes during sex differentiation. A weak staining for P450scc, 3beta-HSD and P450c17, but not P450arom, was present in the tissue surrounding the efferent duct in testes 30 days after hatching. The number of immunoreactive cells as well as the intensity of staining ware increased in testes just before the onset of spermatogenesis. Taken together, these findings suggest that estrogens play an important role in ovarian differentiation in fish. The results of this collaborative research also suggest an important role of estrogens in temperature-dependent sexual differentiation in reptiles. Less
这项国际合作研究将联合收割机结合多种方法和动物系统来研究性别决定和性别分化。特有的夏威夷濑鱼的性别变化为研究性别决定和性别分化机制提供了理想的模式系统。本研究首先采用免疫细胞化学方法检测了隆头鱼在性别转变过程中不同阶段的性腺中P450胆固醇侧链裂解酶(P450 SCC)的表达位点。P450 SCC细胞主要分布于正常女性卵泡膜层。随着雌性发生性别改变,P450 SCC的定位从卵泡膜层转移到卵泡膜层。P450 SCC细胞出现增加的数量中途通过性别变化。然而,性腺产生雌激素的能力在性别变化的早期阶段永久丧失。芳香化酶的mRNA和蛋白质水平,这种酶负责将雄激素转化为雌激素,在睾丸中检测不到 ...更多信息 和过渡性腺在本研究中,我们还利用4种类固醇代谢酶的多克隆抗体,对尼罗罗非鱼性腺性别分化过程中类固醇产生细胞的初始分化和发育进行了免疫细胞化学研究(P450SCC、3beta-HSD、P450c17、P450arom)。P450 SCC,3 β-HSD,P450 C17和P450 arom的免疫反应首次在形态性别分化前几天检查的遗传雌性性腺中变得明显。与此相反,阳性免疫染色的所有四个类固醇生成酶不容易确定在睾丸中的性别分化。P450 arom、P450 SCC、3 β-HSD和P450 c17在出壳后30天的睾丸输出管周围组织中呈弱阳性表达。在精子发生开始前,睾丸中免疫反应细胞的数量和染色强度增加。综上所述,这些发现表明雌激素在鱼类卵巢分化中起着重要作用。这项合作研究的结果还表明,雌激素在爬行动物的温度依赖性性别分化中起着重要作用。少
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Morrey,C.E.,Nakamura,M.,Kobayashi,T.,Grau,E.G.and Nagahama,Y.: "P450scc-like immunoreactivity throughout gonadal restructuring in the protogynous hermaphrodite Thalassoma duperrey" International Journal of Developmental Biology. (will be accepted after mi
Morrey,C.E.、Nakamura,M.、Kobayashi,T.、Grau,E.G. 和 Nagahama,Y.:“在先熟雌雄同体 Thalassoma duperrey 中整个性腺重建过程中的 P450scc 样免疫反应性”国际发育生物学杂志。
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Nakamura,M.,Mariko,T.and Nagahama,Y: "Ultrastructure and in vitro steroidogenesis of the gonads in the protandrous anemonefish Amphiprion frenatu" Japanese Journal of Ichthyology. 41. 47-56 (1994)
Nakamura,M.、Mariko,T. 和 Nagahama,Y:“早熟海葵鱼 Amphiprion frenatu 性腺的超微结构和体外类固醇生成”《日本鱼类学杂志》。
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Tanaka,M.,Fukada,S.,Matsuyama,M.and Nagahama,Y.: "Structure and promoter analysis of the cytochrome P-450 aromatase gene of the teleost fish,medaka (Oryzias latipes)" Journal of Biochemistry. 117. 719-725 (1995)
Tanaka,M.、Fukada,S.、Matsuyama,M. 和 Nagahama,Y.:“硬骨鱼青鳉 (Oryzias latipes) 细胞色素 P-450 芳香酶基因的结构和启动子分析”生物化学杂志。
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Chang, X,T., Kobayashi, T., Kajiura, H., Nakamura, M.and Nagahama, Y.: "Isolation and characterization of the cDNA encoding the tilapia (Oreochromis niloticus) cytochrome P450 aromatase (P450arom) : changes in P450arom mRNA,protein and enzyme activity in
Chang,X,T.,Kobayashi,T.,Kajiura,H.,Nakamura,M.和 Nagahama,Y.:“编码罗非鱼(Oreochromis niloticus)细胞色素 P450 芳香酶(P450arom)的 cDNA 的分离和表征:变化
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Nagahama,Y.: "17α,20β-Dihydroxy-4-pregnen-3-one,a maturation-inducing hormone in fish oocytes : mechanisms of synthesis and action" Steroid. 62. 190-196 (1996)
Nagahama,Y.:“17α,20β-DiHydroxy-4-pregnen-3-one,鱼卵母细胞中的一种成熟诱导激素:合成和作用机制”Steroid 62. 190-196 (1996)。
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NAGAHAMA Yoshitaka其他文献
NAGAHAMA Yoshitaka的其他文献
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{{ truncateString('NAGAHAMA Yoshitaka', 18)}}的其他基金
Molecular mechanisms of sexual plasticity in adult medaka gonads
成年青鳉性腺性可塑性的分子机制
- 批准号:
24370028 - 财政年份:2012
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of ovarian differentiation induced by sex hormones
性激素诱导卵巢分化的分子机制
- 批准号:
20247004 - 财政年份:2008
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Mechanisms of sex determination and gonadal sex differentiation in fish
鱼类性别决定和性腺性别分化的机制
- 批准号:
16086213 - 财政年份:2004
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular Mechanisms of Sex Change in Fish
鱼类性别变化的分子机制
- 批准号:
16370032 - 财政年份:2004
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functions and Mechanisms of Action of the Medaka Sex-Determining Gene
青鳉性别决定基因的功能和作用机制
- 批准号:
14340259 - 财政年份:2002
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of gamete maturation-inducing hormone synthesis
配子成熟诱导激素合成的分子机制
- 批准号:
10440247 - 财政年份:1998
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of oocyte and sperm maturation-inducing hormone
卵母细胞和精子成熟诱导激素的分子机制
- 批准号:
08454266 - 财政年份:1996
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanisms of Germ Cell Formation
生殖细胞形成的分子机制
- 批准号:
07283104 - 财政年份:1995
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular mechanisms of activin B-induced spermatogenesis
激活素 B 诱导精子发生的分子机制
- 批准号:
06454022 - 财政年份:1994
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
General principle involved in the mechanism of oocyte maturation
卵母细胞成熟机制的一般原理
- 批准号:
04044177 - 财政年份:1992
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for international Scientific Research
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