Molecular Mechanisms of Sex Change in Fish
鱼类性别变化的分子机制
基本信息
- 批准号:16370032
- 负责人:
- 金额:$ 9.54万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2004
- 资助国家:日本
- 起止时间:2004 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The gobiid fish, Trimma okinawae, possesses ovarian and testicular tissues simultaneously in its gonad and is able to change sex repeatedly in both directions depending on its social surroundings. As sex change in both directions can be socially manipulated, T.okinawae provides an excellent animal model to investigate molecular mechanisms of sex change. The involvement of gonadotropins in sex change was examined by determining the changes in gonadotropin (FSH and LH) receptor gene (GtHRs) expression in gonads during the onset of sex changes from female to male and male to female. The results were intriguing as the expression of the GtHRs was found to be confined to the active gonad of the corresponding sexual phase. When the sex change was occurring from female to male, initially the ovary had high levels of FSHR and LHR, which eventually went up in the testicular tissue once the fish had realized the fact that it was bigger than the other in the aquarium. Opposite of this scenario was … More observed if another fish bigger than the newly sex-changed male was introduced into this aquarium. Swapping of the gonads started with switching of the GtHR expression that was discernible within 12-24 hrs of the visual cue. Our findings show that the gonads of successive sex changing fish possess the intrinsic mechanism to respond to the social cue differentially. Additionally, this location switching of GtHR expression also could substantiate the importance of hypothalamo-pituitary-gonadotropic axis.The wrasse, Pseudolabrus sieboldi, changes sex from female to male. In this study, to investigate the role of estradio1-17β (E2) and 11-ketotestosterone (11KT) in the conversion between oogenesis and spermatogenesis in the gonad, ovarian and testicular tissues were incubated with E2 or 11-KT respectively using in vitro organ culture system. In the culture of ovarian tissues with 11KT, the cysts of spermatozoa, spermatids, and spermatocytes appeared, while oocytes disappeared. On the contrary, E2 treatment could not organize the cysts of any testicular germ cells in ovarian tissues. In the culture of testicular tissues with 11KT, the cysts of spermatozoa, spermatids, and spermatocytes were observed, while with E2, there was a complete degeneration of testicular tissues. This newly developed organ culture system will provide an excellent in vitro system to study the molecular mechanisms of sex change in fish. Less
虎鱼,Trimma okinawae,在其性腺中同时具有卵巢和睾丸组织,并且能够根据其社会环境在两个方向上反复改变性别。由于两个方向的性别变化都可以被社会操纵,因此冲绳锥虫提供了一个很好的动物模型来研究性别变化的分子机制。通过测定从雌性到雄性和从雄性到雌性的性别变化开始期间性腺中促性腺激素(FSH和LH)受体基因(GtHRs)表达的变化,检查促性腺激素在性别变化中的参与。结果是有趣的,因为发现GtHRs的表达仅限于相应性阶段的活跃性腺。当从雌性到雄性发生性别变化时,最初卵巢具有高水平的FSHR和LHR,一旦鱼意识到它比水族馆中的其他鱼大,睾丸组织中的FSHR和LHR最终会上升。与这种情况相反的是, ...更多信息 观察是否有另一条比刚变性的雄鱼更大的鱼被引入这个水族馆。性腺的交换开始于GtHR表达的转换,这在视觉提示的12-24小时内是可辨别的。我们的研究结果表明,连续性别转换鱼类的性腺具有内在的机制,以响应社会线索的差异。此外,GtHR表达的这种位置转换也证实了下丘脑-垂体-促性腺激素轴的重要性。本研究采用体外器官培养系统,分别用雌二醇(E2)和11-酮睾酮(11-KT)孵育卵巢和睾丸组织,探讨E2和11-KT在性腺卵子发生和精子发生转换中的作用。在卵巢组织中加入11 KT后,精子、精子细胞和精母细胞出现包囊,而卵母细胞消失。相反,E2处理不能组织卵巢组织中的任何睾丸生殖细胞的囊肿。在睾丸组织培养中,11 KT可观察到精子、精子细胞和精母细胞的包囊,而E2则可观察到睾丸组织的完全变性。这种新开发的器官培养系统将为研究鱼类性别变化的分子机制提供一个良好的体外系统。少
项目成果
期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Molecular mechanisms of sex determination and gonadal sex differentiation in fish
- DOI:10.1007/s10695-006-7590-2
- 发表时间:2005-04-01
- 期刊:
- 影响因子:2.9
- 作者:Nagahama, Y.
- 通讯作者:Nagahama, Y.
Characterization of two types of cytochrome P450 aromatase in serial-sex changing gobiid fish, Trimma okina wae.
连续性别改变的戈壁鱼 Trimma okina wae 中两种细胞色素 P450 芳香酶的特征。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Kobayashi;Y.;Kobayashi;T.;Nakamura;M.;Sunobe T.;Morrey;C.E.;Suzuki;N.;Nagahama;Y.
- 通讯作者:Y.
Gonadal structure and P450scc and 3β-HSD immunoreactivity in the gobiid fish Trimma okinawae during bi-directed sex change.
双向性变期间戈壁鱼 Trimma 冲绳的性腺结构和 P450scc 和 3β-HSD 免疫反应性。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Sunobe;T. et al.
- 通讯作者:T. et al.
Characterization of two types of cytochrome P450 aromatase in the serial-sex changing gobiid fish, Trimma okinawae
连续变性戈壁鱼 Trimma Okinawae 中两种细胞色素 P450 芳香酶的表征
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Kobayashi;Y. et al.
- 通讯作者:Y. et al.
Gonadal structure of the serial‐sex changing gobiid fish Trimma okinawae
- DOI:10.1111/j.1440-169x.2004.00774.x
- 发表时间:2005-01
- 期刊:
- 影响因子:4.6
- 作者:Yasuhisa Kobayashi;T. Sunobe;Tohru Kobayashi;Y. Nagahama;Masaru Nakamura
- 通讯作者:Yasuhisa Kobayashi;T. Sunobe;Tohru Kobayashi;Y. Nagahama;Masaru Nakamura
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NAGAHAMA Yoshitaka其他文献
NAGAHAMA Yoshitaka的其他文献
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{{ truncateString('NAGAHAMA Yoshitaka', 18)}}的其他基金
Molecular mechanisms of sexual plasticity in adult medaka gonads
成年青鳉性腺性可塑性的分子机制
- 批准号:
24370028 - 财政年份:2012
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of ovarian differentiation induced by sex hormones
性激素诱导卵巢分化的分子机制
- 批准号:
20247004 - 财政年份:2008
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Mechanisms of sex determination and gonadal sex differentiation in fish
鱼类性别决定和性腺性别分化的机制
- 批准号:
16086213 - 财政年份:2004
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Functions and Mechanisms of Action of the Medaka Sex-Determining Gene
青鳉性别决定基因的功能和作用机制
- 批准号:
14340259 - 财政年份:2002
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of gamete maturation-inducing hormone synthesis
配子成熟诱导激素合成的分子机制
- 批准号:
10440247 - 财政年份:1998
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of oocyte and sperm maturation-inducing hormone
卵母细胞和精子成熟诱导激素的分子机制
- 批准号:
08454266 - 财政年份:1996
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanisms of Germ Cell Formation
生殖细胞形成的分子机制
- 批准号:
07283104 - 财政年份:1995
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular Mechanisms of Sex Determination and Differentiation
性别决定和分化的分子机制
- 批准号:
06044235 - 财政年份:1994
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for international Scientific Research
Molecular mechanisms of activin B-induced spermatogenesis
激活素 B 诱导精子发生的分子机制
- 批准号:
06454022 - 财政年份:1994
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
General principle involved in the mechanism of oocyte maturation
卵母细胞成熟机制的一般原理
- 批准号:
04044177 - 财政年份:1992
- 资助金额:
$ 9.54万 - 项目类别:
Grant-in-Aid for international Scientific Research
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