Porcine otitis is a novel mycoplasmal disease

猪中耳炎是一种新型支原体病

• 批准号: 06454130
• 负责人: UMEMURA Takashi
• 金额: $ 4.61万
• 依托单位: Faculty of Agriculture, Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454130/
• 关键词: Pigs; Otitis media; Mycoplasmosis; Mycoplasma hyorhinis; Pathogenesis; Experimental pathology; Mycoplasma hyorhinis; 耳管炎; マイコプラズマ ハイオリニス

The ear of 479 pigs from 8 commercial farms, ranging age from 1 day to 1 year old were pathologically and microbiologically examined. 364(76.0%) of 479 pigs were affected wit otitis. Eustachitis was the most common and preceded an inflammation of other sites of the ear, and an acute eustachitis occurred from as early as 1 week of life. Common bacterial habitants of the upper respiratory tracts such as Pasteurella multocida and Actinomyces pyogenes were the most frequent isolates from the exudate in the tympanic cavity or in the tympanic bulla. Immunohistochemical examination of frozen cryostat sections revealed Mycoplasma hyorhinis (Mhr) antigens on the luminal surface of the eustachian epithelia in 14 (50.0%) of 28 piglets examined. Mhr was isolated from the auditory tubes and tympanic cavities of 19 (67.9%) and 16 (57.1%) of 28 piglets, respectively. All the pigs positive for Mhr had an acute eustachitis. Experimental reproduction of otitis was performed using seventeen, 17- to 22-day-old, SPF piglets. Mhr inoculated in the middle ear multiplied and induced transient, purulent-catarrhal inflammation of the ear. From these results, it was concluded that porcine otitis media might be caused primarily by Mhr infection and be a novel mycoplasmal disease.

对8个商品猪场479头1日龄至1日龄猪的耳部进行了病理学和微生物学检查。在479头猪中，有364头(76.0%)患了中耳炎。咽鼓炎是最常见的，先于耳朵其他部位的炎症，急性咽鼓炎最早发生在出生后1周。从鼓室或鼓泡渗出液中分离到的细菌以多杀性巴氏杆菌、化脓性放线菌等上呼吸道常见细菌最多见。28头受检仔猪中，14头(50.0%)咽鼓管上皮细胞腔表面有猪鼻支原体(MHR)抗原。从28头仔猪的耳道和鼓室分别分离出MHR 19头(67.9%)和16头(57.1%)。所有MHR阳性的猪都有急性咽鼓炎。用17-22日龄SPF仔猪进行了中耳炎的实验复制。在中耳接种MHR可引起耳部一过性、化脓性卡他性炎症。这些结果表明，猪中耳炎可能主要由MHR感染引起，是一种新的支原体疾病。

项目成果

Morita,T.: "Demonstration of Mycoplasma hyorhinis as a possible pathogen for porcine otitis media" Veterinary Pathology. 32. 107-111 (1995)

Morita,T.：“证明猪鼻支原体是猪中耳炎的可能病原体”兽医病理学。

梅村孝司: "豚の耳炎は新しいマイコプラズマ性疾患か?" ふぉ-なす. 13. 1-6 (1995)

Takashi Umemura：“猪中耳炎是一种新的支原体疾病吗？”Fornas 13. 1-6 (1995)

Umemura, T., Morita, T., Kaji, N., Shimada, A., Kazama, S.and Yagihashi, T.: "Mycoplasma hyorhinis is a primary pathogen for porcine otitis media" Japanese Journal of Mycoplasmology. 21・22. 12-115 (1995)

Umemura, T.、Morita, T.、Kaji, N.、Shimada, A.、Kazama, S. 和 Yagihashi, T.：“猪鼻支原体是猪中耳炎的主要病原体”日本支原体学杂志 21・22。 .12-115 (1995)

Morita, T.: "Detection of Mycoplasma hyorhinis in porcine eustachitis" Journal of Veterinary Medical Science. 55. 475-477 (1993)

Morita, T.：“猪咽鼓炎中猪鼻支原体的检测”《兽医医学科学杂志》。

Shimada, A., Adachi, T., Umemura, T., Kohno, K., Sakaguchi, Y., and Itakura, C.: "A pathologic and bacteriologic study on otitis media in swine" Veterinary Pathology. 29. 337-342 (1992)

Shimada, A.、Adachi, T.、Umemura, T.、Kohno, K.、Sakaguchi, Y. 和 Itakura, C.：“猪中耳炎的病理学和细菌学研究”兽医病理学。