Pathogenicity and its mechanism of Mycoplasma hyorhinis

猪鼻支原体的致病性及其机制

基本信息

批准号：
08456159
负责人：
UMEMURA Takashi
金额：
$ 4.86万
依托单位：
HOKKAIDO UNIVERSITY (1997)Tottori University (1996)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456159/
关键词：
SWINE OTITIS MEDIA EUSTACHITIS MYOPLASMA MYCOPLASMA HYORHINIS PASTEURELLA MULTOCIDA Pasteurella multocida 経鼻接種

项目摘要

(l)Induction of otitis media by intratympanic inoculation of Mycoplasma hyorhinisFifteen SPF piglets, known to be free of Mycoplasma, were allocated in three groups : non-treatment control, intratympanic inoculation of Mycoplasma hyorhinis (Mhr) , and sham operated. Mild to moderate inflammation of the auditory tube and tympanic cavity first appeared at 7 days post inoculation (DPI) in Mhr-inoculated piglets. The degree of the inflammation was aggravated in the piglets sacrificed at 14 DPI.Immunohistochemistry and electron microscopical examinations revealed Mhr on the luminal surface of the auditory tube and tympanic cavity. The ear lesions weakened at 25 DPI.Mhr was isolated from the tympanic cavity from 0 to 14 DPI,but the amounts decreased at 25 DPI.The present experiment indicated that Mhr inoculated into the tympanic cavity could cause a self-limited otitis media in SPF piglets.(2) Induction of otitis media by intranasal inoculation of MhrEleven SPF piglets were allocated in the following 4 groups : negative control, intranasal inoculation of Mhr, intranasal inoculation of Pasteurella multocida (Pm) , and intranasal inoculation of Mhr and Pm. Subacute eustachitis was found in 10 of 12 middle ear of 6 piglets inoculated with Mhr. Immunohistochemistry and ultrastructure confirmed the infection of Mhr in the les ion. None of the piglets inoculated only with Pm showed otitis and synergistic action of Mhr and Pm was not found.The present experiment indicated that intranasally infected Mhr, but not Pm alone could cause eustachitis.These two experiments substantiated our previous hypothesis that Mhr is the primary cause of otitis media of piglets

（l）分配了三组：非治疗控制，甲状腺内胰蛋白酶内接种菌根（MHR）（MHR）（MHR）（MHR）和Shams Shams operated。听觉管和鼓膜腔的轻度至中度炎症首次在接种后7天出现在MHR接种的小猪中。在14 dpi处死的小猪中加剧了炎症程度。免疫组织化学和电子显微镜检查揭示了在听觉管和鼓膜腔的腔表面上的MHR。在25 dpi.mhr时从鼓腔从0到14 dpi分离的耳朵病变，但在25 dpi时的量减少。目前的实验表明，将MHR接种到鼓膜腔中可能导致SPF Piglet中的spf Piglets in Interan insansemition。在以下四组中分配：阴性对照，MHR的鼻内接种，鼻内接种Multocida（PM）（PM）以及MHR和PM的鼻内接种。在接种MHR的6个中耳中，在12个中耳中的10个中耳中发现了亚急性的脑炎。免疫组织化学和超微结构证实了MHR在LES离子中的感染。没有发现仅接种PM的小猪表现出中耳炎和MHR和PM的协同作用。目前的实验表明，鼻内感染的MHR，但仅单独使用PM，但不单独引起pm eustachitis。