Pathogenicity and its mechanism of Mycoplasma hyorhinis

猪鼻支原体的致病性及其机制

• 批准号: 08456159
• 负责人: UMEMURA Takashi
• 金额: $ 4.86万
• 依托单位: HOKKAIDO UNIVERSITY (1997)Tottori University (1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456159/
• 关键词: SWINE; OTITIS MEDIA; EUSTACHITIS; MYOPLASMA; MYCOPLASMA HYORHINIS; PASTEURELLA MULTOCIDA; Pasteurella multocida; 経鼻接種

(l)Induction of otitis media by intratympanic inoculation of Mycoplasma hyorhinisFifteen SPF piglets, known to be free of Mycoplasma, were allocated in three groups : non-treatment control, intratympanic inoculation of Mycoplasma hyorhinis (Mhr) , and sham operated. Mild to moderate inflammation of the auditory tube and tympanic cavity first appeared at 7 days post inoculation (DPI) in Mhr-inoculated piglets. The degree of the inflammation was aggravated in the piglets sacrificed at 14 DPI.Immunohistochemistry and electron microscopical examinations revealed Mhr on the luminal surface of the auditory tube and tympanic cavity. The ear lesions weakened at 25 DPI.Mhr was isolated from the tympanic cavity from 0 to 14 DPI,but the amounts decreased at 25 DPI.The present experiment indicated that Mhr inoculated into the tympanic cavity could cause a self-limited otitis media in SPF piglets.(2) Induction of otitis media by intranasal inoculation of MhrEleven SPF piglets were allocated in the following 4 groups : negative control, intranasal inoculation of Mhr, intranasal inoculation of Pasteurella multocida (Pm) , and intranasal inoculation of Mhr and Pm. Subacute eustachitis was found in 10 of 12 middle ear of 6 piglets inoculated with Mhr. Immunohistochemistry and ultrastructure confirmed the infection of Mhr in the les ion. None of the piglets inoculated only with Pm showed otitis and synergistic action of Mhr and Pm was not found.The present experiment indicated that intranasally infected Mhr, but not Pm alone could cause eustachitis.These two experiments substantiated our previous hypothesis that Mhr is the primary cause of otitis media of piglets

（l）通过鼓室内接种猪鼻支原体诱导中耳炎将15只已知不含支原体的SPF仔猪分为三组：非治疗对照组、鼓室内接种猪鼻支原体（Mhr）组和假手术组。在接种Mhr的仔猪中，在接种后7天（DPI）首次出现轻度至中度的咽鼓管和鼓室炎症。免疫组织化学和电镜检查显示，在14 DPI时，咽鼓管和鼓室腔表面有Mhr。0 ~ 14 DPI时，Mhr从鼓室中分离出来，但25 DPI时，Mhr的量减少，说明Mhr接种于SPF仔猪鼓室可引起自限性中耳炎。(2)通过鼻内接种Mhr诱导中耳炎将11头SPF仔猪分配到以下4组：阴性对照组、鼻内接种Mhr组、鼻内接种多杀性巴氏杆菌（Pm）组以及鼻内接种Mhr和Pm组。6头接种Mhr的仔猪12只中耳中有10只发生了亚急性咽鼓管炎。免疫组化和超微结构证实了Mhr的感染。结果表明，Mhr与Pm均未引起仔猪中耳炎，也未发现Mhr与Pm的协同作用。本实验表明，Mhr经鼻感染可引起仔猪中耳炎，而Pm单独感染则不能引起。这两个实验证实了Mhr是引起仔猪中耳炎的主要原因的假说

项目成果

Morita T., Sasaki, A., Kaji, N., Shimada, A., Kazama, S., Yagihashi, T., and Umemura, T.: "Intratympanic inoculation of Mycoplasma hyorhinis causes temporary otitis media in specific pathogen free piglets." American Journal of Veterinary Research. (in pre

Morita T.、Sasaki, A.、Kaji, N.、Shimada, A.、Kazama, S.、Yagihashi, T. 和 Umemura, T.：“鼓室内接种猪鼻支原体会导致无特定病原体仔猪暂时性中耳炎

Morita Takehito: "Intratympanic inoculation of Mycoplasma hyorhinis causes temporary ofifis media in specific pathogen free piglefs" American Journal of Veterinary Research. (in press). (1998)

森田武人：“鼓室内接种猪鼻支原体会导致无特定病原体的仔猪出现暂时性中耳炎”《美国兽医研究杂志》。

Morita Takehito: "Intratympanic inoculation of Mycoplasma hyorhinis causes temporary otitis media in specitic pathogen free piglets" American Journal of Veterinary Research. (in press). (1998)

Morita Takehito：“鼓室内接种猪鼻支原体会导致无特定病原体的仔猪出现暂时性中耳炎”《美国兽医研究杂志》。