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Pathogenicity and its mechanism of Mycoplasma hyorhinis

猪鼻支原体的致病性及其机制

基本信息

批准号：
08456159
负责人：
UMEMURA Takashi
金额：
$ 4.86万
依托单位：
HOKKAIDO UNIVERSITY (1997)Tottori University (1996)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456159/
关键词：
SWINE OTITIS MEDIA EUSTACHITIS MYOPLASMA MYCOPLASMA HYORHINIS PASTEURELLA MULTOCIDA Pasteurella multocida 経鼻接種

项目摘要

(l)Induction of otitis media by intratympanic inoculation of Mycoplasma hyorhinisFifteen SPF piglets, known to be free of Mycoplasma, were allocated in three groups : non-treatment control, intratympanic inoculation of Mycoplasma hyorhinis (Mhr) , and sham operated. Mild to moderate inflammation of the auditory tube and tympanic cavity first appeared at 7 days post inoculation (DPI) in Mhr-inoculated piglets. The degree of the inflammation was aggravated in the piglets sacrificed at 14 DPI.Immunohistochemistry and electron microscopical examinations revealed Mhr on the luminal surface of the auditory tube and tympanic cavity. The ear lesions weakened at 25 DPI.Mhr was isolated from the tympanic cavity from 0 to 14 DPI,but the amounts decreased at 25 DPI.The present experiment indicated that Mhr inoculated into the tympanic cavity could cause a self-limited otitis media in SPF piglets.(2) Induction of otitis media by intranasal inoculation of MhrEleven SPF piglets were allocated in the following 4 groups : negative control, intranasal inoculation of Mhr, intranasal inoculation of Pasteurella multocida (Pm) , and intranasal inoculation of Mhr and Pm. Subacute eustachitis was found in 10 of 12 middle ear of 6 piglets inoculated with Mhr. Immunohistochemistry and ultrastructure confirmed the infection of Mhr in the les ion. None of the piglets inoculated only with Pm showed otitis and synergistic action of Mhr and Pm was not found.The present experiment indicated that intranasally infected Mhr, but not Pm alone could cause eustachitis.These two experiments substantiated our previous hypothesis that Mhr is the primary cause of otitis media of piglets

(l)通过鼓室内接种猪鼻支原体诱导中耳炎将已知不含支原体的15头SPF小猪分配成三组：未治疗对照、鼓室内接种猪鼻支原体(Mhr)和假手术组。接种 Mhr 的仔猪在接种后 7 天 (DPI) 首次出现耳管和鼓室的轻度至中度炎症。 14 DPI处死仔猪炎症程度加重。免疫组织化学和电镜检查显示耳管和鼓腔管腔表面存在Mhr。 25 DPI时耳部病变减弱。0～14 DPI时从鼓室分离出Mhr，但25 DPI时数量减少。本实验表明，鼓腔接种Mhr可引起SPF仔猪自限性中耳炎。(2)鼻内接种Mhr11 SPF仔猪诱发中耳炎分为以下4组：阴性对照、鼻内接种Mhr、鼻内接种多杀性巴氏杆菌(Pm)、以及鼻内接种Mhr和Pm。接种Mhr.的6头仔猪中，12头中耳中的10头发现亚急性咽鼓炎。免疫组化和超微结构证实病灶内存在Mhr感染。单独接种Pm的仔猪均未出现中耳炎，且未发现Mhr和Pm的协同作用。本实验表明鼻内感染Mhr可引起咽鼓炎，而单独接种Pm则不会。这两个实验证实了我们之前的假设，即Mhr是仔猪中耳炎的主要原因。