PATHOGENESIS OF INFLUENZAL ENCEPHALITIS/ENCEPHALOPATHY

流感脑炎/脑病的发病机制

• 批准号: 14360182
• 负责人: UMEMURA Takashi
• 金额: $ 9.54万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360182/
• 关键词: Influenza; Influenza virus; Influenzal encephalopathy; Aujezky virus; Transneural Spread; Viral encephalitis; Intrathecal vaccination; Reye's syndrome; トリインフルエンザウイルス; H5N1; 持続感染; 解熱剤; 経神経感染; ボルタレン; 迷走神経切除; アスピリン; インフルエンザ脳炎; 脳症

Demonstration of neural spread of influenza virus(IFV)Intranasally inoculated highly pathogenic IFV to mice invaded medulla oblongata and thoracic cord possibly via vagal and/or sympathetic nerves (Vet Microbiol,2003). Inoculation of IFV to unilaterally vagectomized mice demonstrated the virus invades brain stem through vagal nerve(Vet Pathol,2004). Transneural transmission of IFV was examined using compartmentalized cultures of neurons from mouse dorsal root ganglia, the results were compared with those obtained using the pseudorabies virus. The results indicated that IFV can be spread transneurally and the transmission is independent of axonal transport using microtubules (J Gen Viral,2005).Persistent infection of IFVViral antigen, viral genomes and encephalitis persisted for not longer than 48 days in mice brain after intranasal inoculation of IFV. However the virus was not recovered from the brain.Experimental reproduction of Reyes' s syndromeThe cause of Reye's syndrome, acute enc … More ephalopathy of childhood is suspected of IFV infection and antipyretic treatment. The effects of antipyretics (aspirin and voltaren) on avian and murine models of influenzal encephalitis/encephalopathy were examined. Antipyretic treatment did not affect murine model (transneural spread model of IFV), whereas the treatment enhanced avian encephalitis/encephalopathy (hematogeneous spread model of IFV). Voltaren exaggerated the lesion more prominently than aspirin(J Vet Med Sci,2004).Intrathecal immunizationInactivated IFV or Aujezky virus were inoculated intrathecally (inoculation into cerebrospinal fluid) or subcutaneously in rabbits and mice. Antibody in cerebrospinal fluid was raised only by intrathecal immunization. Intrathecal immunization using inactivated Aujezky virus protected mice completely from clinical signs, pathological changes and death due to intramuscular inoculation of Aujezky virus. The results indicated the efficacy of intrathecal immunization against the viruses which invade central nervous system from invasion sites through the peripheral nerves. Less

影响力（IFV）内汇总接种了高度致病性IFV对小鼠的高度致病性IFV的神经元扩散的证明，通过迷走神经和/或交感神经可能会侵入延髓和胸腔（Vet Microtobiol，2003）。对单方面对照小鼠的IFV接种，证明了通过迷走神经的病毒感染脑干（Vet Pathol，2004）。使用小鼠背根神经节神经元的分室化培养物检查了IFV的跨性传递，将结果与使用假性药物病毒获得的结果进行了比较。结果表明，IFV可以通过微管传播，传播与轴突转运无关（J Gen Viral，2005年）。在IFV肠内接种后，小鼠的IFV病毒抗原，病毒基因组和脑炎持续不超过48天。然而，该病毒未从大脑中回收。雷耶斯综合症的实验性繁殖是雷耶综合征的原因，急性结束……童年时期更多的表达症状是IFV感染和抗染料治疗。检查了抗染料药（阿司匹林和伏尔替伦）对影响脑炎/脑病的禽类和鼠模型的影响。抗染料治疗不会影响鼠模型（IFV的跨性传播模型），而治疗增强了禽脑炎/脑病（IFV的血压扩散模型）。与阿司匹林相比，伏尔氏素对病变更为突出（J Vet Med Sci，2004年）。在兔和小鼠中接种了肠内摄入的IFV或Aujezky病毒的Intrathecal免疫抑制激活或Aujezky病毒被接种。脑脊液中的抗体仅通过鞘内免疫抑制饲养。鞘内免疫抑制使用灭活的Aujezky病毒完全保护小鼠免受临床体征，病理变化和由于肌内接种而导致Aujezky病毒引起的死亡。结果表明，鞘内免疫对通过周围神经系统从侵袭部位侵入中枢神经系统的病毒的有效性。较少的

项目成果

The vagus nerve is one route of transneural invasion for intranasally inoculated influenza A virus in mice

• DOI: 10.1354/vp.41-2-101
• 发表时间: 2004-03-01
• 期刊: VETERINARY PATHOLOGY
• 影响因子: 2.4
• 作者: Matsuda, K;Park, CH;Umemura, T
• 通讯作者: Umemura, T

H.Tanaka: "Neurotropism of the 1997 Hong Kong H5N1 influenza virus in mice"Veterinary Microbiology. 95. 1-13 (2003)

H.Tanaka：“1997年香港H5N1流感病毒在小鼠体内的向神经性”兽医微生物学。

The effects of antipyretics on influenza virus encephalitis in mice and chicks.

• DOI: 10.1292/jvms.65.1185
• 发表时间: 2003-11
• 期刊: The Journal of veterinary medical science
• 作者: Y. Sunden;C. Park;K. Matsuda;Akiko Anagawa;Takashi Kimura;K. Ochiai;H. Kida;T. Umemura

Persistence of viral RNA segments in the central nervous system of mice after recovery from acute influenza A virus infection

急性甲型流感病毒感染恢复后小鼠中枢神经系统中病毒RNA片段的持续存在

• 发表时间: 2003
• 期刊: Vet.Microbiol. 97
• 作者: Park C.H.

K.Matsuda: "The vagus nerve is one route of transneural invasion for intranasally inoculatad influenza A virus in mice"Veterinary Pathology. 41(in press). (2004)

K.Matsuda：“迷走神经是小鼠鼻内接种甲型流感病毒经神经侵入的一种途径”《兽医病理学》。