Evaluation of Contact Sensitivity to Occupational Chemicals Using Cytokine Profile Analysis

过敏性接触性皮炎是通过细胞免疫反应介导的，通常在某些职业中由于暴露于某些反应性化学物质而观察到。预测接触性化学物质的致敏潜力对预防职业性变应性接触性皮炎具有重要意义。本研究的目的是发展的方法预测接触敏感性的化学品使用细胞因子谱分析。分别用DNCB、OXAZ和PCL局部致敏Balb/C雌性小鼠，然后用相同致敏剂以非刺激性剂量诱发变应性接触性皮炎。采用逆转录聚合酶链反应检测诱导期和激发期淋巴器官和病变皮肤组织中细胞因子mRNA的表达。结果表明，在诱导期，在引流淋巴结中仅观察到IL-2、IL-4、IL-10、IL-12 p35、IL-12 p40和IFN-γ的弱mRNA信号，而这些细胞因子mRNA在DNCB和OXAZ致敏后强烈增强。此外，虽然IL-5和IL-13的mRNA不可检测，但它们在DNCB和OXAZ处理后被诱导。发现在激发阶段，IL-12和IFN-γ mRNA的表达在患有变应性接触性皮炎的小鼠中上调，而IL-2、IL-4和IL-10 mRNA保持不变。虽然IL-2、IL-4和IFN-γ的mRNA在正常小鼠皮肤中被微弱地发现或检测不到，但它们在患病皮肤部位大量增加。因此，我们的研究结果表明，同时检测皮肤和淋巴结细胞因子谱可能有助于识别职业化学品的接触敏感性。

Allergic contact dermatisi is mediated through a cell-immune responses and are generally observed among the certain occupations due to exposure to some reactive chemicals. For prevention of occupational allergic contact dermatitis, It is important to predict the contact sensitizing potentials to chemicals. This study was aimed at developing the methods for predicting contact sensitivity to chemicals using cytokine profile analysis. Balb/C femal mice were topically sensitized with DNCB,OXAZ and PCL,respectively, then allergic contact dermatitis was induced by the same sensitizer at non-irritant dose. The cytokine mRNAs at lymph organs and diseased skin sites were examined by reverse-trancription-polymerase chain reaction both in induction and challenge phases. The results have demonstrated that, at induction phase, only weak mRNA signals for IL-2, IL-4, IL-10, Ilp35, IL-12p40 and IFN-gamma were observed the draining lymph nodes whereas these cytokine mRNAs were strongly enhanced following sensitization with DNCB and OXAZ.Moreover, though the mRNAa of IL-5 and IL-13 were undetectable, but they were induced following treatment with DNCB and OXAZ.Furthermore, it was found that, at challenge phase, the expressions of IL-12 and IFN-gamma mRNAs were up-regulated in mice with allergic contact dermatitis whereas IL-2, IL-4 and IL-10 mRNAs remains unchanged. Although the mRNAs for IL-2, IL-4 and IFN-gamma were weakly found or undetectable at normal murine skin, they were largely augmented at the diseased skin sites. Therefore, our results suggest that simutanous dtection of cytokine profiles at skin and lymph nodes may be useful for indentify the contact sensitivity to occupational chemicals.