Evaluation of Contact Sensitivity to Occupational Chemicals Using Cytokine Profile Analysis

过敏性接触性皮炎是通过细胞免疫反应调节的，由于暴露于一些活性化学物质中，通常在某些职业中观察到。为了预防职业性过敏性接触性皮炎，预测接触者对化学物质的致敏潜力是很重要的。本研究旨在开发利用细胞因子谱分析预测接触化学物质敏感性的方法。分别用DNCB、OXAZ和PCL局部致敏BALB/C雌性小鼠，然后用相同的致敏剂在无刺激性剂量下诱发变态反应性接触性皮炎。用逆转录-聚合酶链式反应检测诱导和激发阶段淋巴器官和病变皮肤部位的细胞因子mRNAs。结果表明，在诱导期，IL-2、IL-4、IL-10、ILp35、IL-12p40和干扰素-γ在引流淋巴结中只有微弱的mRNA信号，而DNCB和OXAZ致敏后这些细胞因子的mRNAs明显增强；IL-5和IL-13的mRNAa未被检测到，但经DNCB和OXAZ治疗后可被诱导。此外，在激发阶段，过敏性皮炎小鼠IL-12和干扰素-γmRNAs的表达上调，而IL-2、IL-4和IL-10mRNAs的表达没有变化。虽然IL-2、IL-4和干扰素-γ的mRNAs在正常小鼠皮肤中很弱或检测不到，但在疾病皮肤部位它们的表达显著增强。因此，我们的结果表明，同时检测皮肤和淋巴结处的细胞因子谱可能有助于确定接触者对职业化学物质的敏感性。

Allergic contact dermatisi is mediated through a cell-immune responses and are generally observed among the certain occupations due to exposure to some reactive chemicals. For prevention of occupational allergic contact dermatitis, It is important to predict the contact sensitizing potentials to chemicals. This study was aimed at developing the methods for predicting contact sensitivity to chemicals using cytokine profile analysis. Balb/C femal mice were topically sensitized with DNCB,OXAZ and PCL,respectively, then allergic contact dermatitis was induced by the same sensitizer at non-irritant dose. The cytokine mRNAs at lymph organs and diseased skin sites were examined by reverse-trancription-polymerase chain reaction both in induction and challenge phases. The results have demonstrated that, at induction phase, only weak mRNA signals for IL-2, IL-4, IL-10, Ilp35, IL-12p40 and IFN-gamma were observed the draining lymph nodes whereas these cytokine mRNAs were strongly enhanced following sensitization with DNCB and OXAZ.Moreover, though the mRNAa of IL-5 and IL-13 were undetectable, but they were induced following treatment with DNCB and OXAZ.Furthermore, it was found that, at challenge phase, the expressions of IL-12 and IFN-gamma mRNAs were up-regulated in mice with allergic contact dermatitis whereas IL-2, IL-4 and IL-10 mRNAs remains unchanged. Although the mRNAs for IL-2, IL-4 and IFN-gamma were weakly found or undetectable at normal murine skin, they were largely augmented at the diseased skin sites. Therefore, our results suggest that simutanous dtection of cytokine profiles at skin and lymph nodes may be useful for indentify the contact sensitivity to occupational chemicals.