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Evaluation of Contact Sensitivity to Occupational Chemicals Using Cytokine Profile Analysis

使用细胞因子谱分析评估对职业化学品的接触敏感性

基本信息

批准号：
06454230
负责人：
MATSUSHITA Toshio
金额：
$ 3.65万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454230/
关键词：
Occupatinal Chemicals Allergic Contact Dermatitis Contact Sensitivity Cytokine mRNA Skin Lymph Organ RT-PCR Measure

项目摘要

Allergic contact dermatisi is mediated through a cell-immune responses and are generally observed among the certain occupations due to exposure to some reactive chemicals. For prevention of occupational allergic contact dermatitis, It is important to predict the contact sensitizing potentials to chemicals. This study was aimed at developing the methods for predicting contact sensitivity to chemicals using cytokine profile analysis. Balb/C femal mice were topically sensitized with DNCB,OXAZ and PCL,respectively, then allergic contact dermatitis was induced by the same sensitizer at non-irritant dose. The cytokine mRNAs at lymph organs and diseased skin sites were examined by reverse-trancription-polymerase chain reaction both in induction and challenge phases. The results have demonstrated that, at induction phase, only weak mRNA signals for IL-2, IL-4, IL-10, Ilp35, IL-12p40 and IFN-gamma were observed the draining lymph nodes whereas these cytokine mRNAs were strongly enhanced following sensitization with DNCB and OXAZ.Moreover, though the mRNAa of IL-5 and IL-13 were undetectable, but they were induced following treatment with DNCB and OXAZ.Furthermore, it was found that, at challenge phase, the expressions of IL-12 and IFN-gamma mRNAs were up-regulated in mice with allergic contact dermatitis whereas IL-2, IL-4 and IL-10 mRNAs remains unchanged. Although the mRNAs for IL-2, IL-4 and IFN-gamma were weakly found or undetectable at normal murine skin, they were largely augmented at the diseased skin sites. Therefore, our results suggest that simutanous dtection of cytokine profiles at skin and lymph nodes may be useful for indentify the contact sensitivity to occupational chemicals.

过敏性接触性皮肤病是通过细胞免疫反应介导的，并且通常在某些职业中由于暴露于某些反应性化学物质而观察到。为了预防职业性过敏性接触性皮炎，预测化学品的接触致敏潜力非常重要。本研究旨在开发使用细胞因子谱分析预测对化学品的接触敏感性的方法。分别用DNCB、OXAZ和PCL局部致敏Balb/C雌性小鼠，然后用相同致敏剂以非刺激剂量诱导过敏性接触性皮炎。在诱导和攻击阶段，通过逆转录聚合酶链反应检查淋巴器官和患病皮肤部位的细胞因子 mRNA。结果表明，在诱导期，引流淋巴结仅观察到微弱的 IL-2、IL-4、IL-10、Ilp35、IL-12p40 和 IFN-gamma mRNA 信号，而这些细胞因子 mRNA 在 DNCB 和 OXAZ 致敏后强烈增强。此外，虽然 IL-5 和 IL-13 的 mRNA 检测不到，但它们 DNCB和OXAZ治疗后诱导产生。此外，研究发现，在攻击期，过敏性接触性皮炎小鼠中IL-12和IFN-γ mRNA的表达上调，而IL-2、IL-4和IL-10 mRNA的表达保持不变。尽管IL-2、IL-4和IFN-γ的mRNA在正常小鼠皮肤中微弱地发现或检测不到，但它们在患病皮肤部位大量增加。因此，我们的结果表明，同时检测皮肤和淋巴结的细胞因子谱可能有助于确定对职业化学品的接触敏感性。