Detection of hepatitis viruses in liver tissues and clinical characteristics of them.

肝组织中肝炎病毒的检测及其临床特征。

• 批准号: 06454388
• 负责人: TSUZUKI Toshiharu
• 金额: $ 4.03万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454388/
• 关键词: liver tissue; hepatitis virus; HCC; clinical characteristics; 肝細胞材癌

Recent studies suggest that HCV has many subtypes and that each clinical property such as the response to the treatment for the hepatitis and natural course of diesase is different. In this studythe relation between HCV or HBV and hapatocarcinogenesis was investigated. Liver tissues from patients with hapatocellular carcinoma (HCC) and HCV-antibody positive patients without HCC were examined. Detection of HCV subtypes was performed by RT-nested-PCR for each subtype : type-I,II,III and IV.HBV was detected by PCR.Results were as follows : 1. Most of patient swith HCC was detected HCV or HBV in liver tissues. 2. Virus marker in sera was not always coincided with the presence of virus in liver tissues. 3. Detectionrate of HCV RNA was hegher in non-tumro tissues than in tumor tissues. 4. There was no significant difference in clinical characteristics of HCC between HCV subtypes. 5. The liver function in patients detected several HCV subtypes was more disturbed than those detected single subtype. 6. There was no difference of HCV subtypes between patients with HCV and HCV-antibody positive patients without HCC.

近年来的研究表明，丙型肝炎病毒有许多亚型，每种亚型的临床特征如对肝炎治疗的反应和自然病程是不同的。本研究探讨了HCV、HBV与肝癌发生的关系。肝细胞癌（HCC）患者和HCV抗体阳性的非HCC患者的肝组织进行了检查。采用逆转录-巢式-聚合酶链反应（RT-nested-PCR）检测HCV的I、II、III、IV型亚型，同时采用聚合酶链反应（PCR）检测HBV。HCC患者肝组织中大多数检出HCV或HBV。2.血清中病毒标志物与肝组织中病毒的存在并不总是一致的。3. HCVRNA在非肿瘤组织中的检出率高于肿瘤组织。4. HCV亚型间HCC的临床特征无明显差异。5.同时检测多种亚型的患者肝功能紊乱程度明显高于单一亚型的患者。6. HCV感染者与HCV抗体阳性的非HCC患者之间的HCV亚型无差异。

项目成果

Ozawa S.,et al.: "Primary structures and properties of two related forms of aryl sulfotranceferase in liver." Pharmacogenetics. 5. 135-140 (1995)

Ozawa S.,et al.：“肝脏中两种相关形式的芳基磺胺转移酶的主要结构和特性。”

Tsuzuki, T., et al.: "How far should we treat metastatic liverc ancer? Report of three long-term survivors" J. Hepato. Biliary Pancreatic Surg.3. 51-53 (1996)

Tsuzuki, T. 等人：“我们应该治疗转移性肝癌到什么程度？三位长期幸存者的报告”J. Hepato。

都築俊治,ほか: "術後合併症とその治療 術後肝障害" 外科治療. 72. 576-578 (1995)

Toshiharu Tsuzuki 等人：“术后并发症及其治疗 术后肝损伤” 外科治疗。 72. 576-578 (1995)

Ozawa S., et.al.: "Primary structures and properties of two related forms of aryl sulfotranseferase in liver." Pharmacogenetics. 5. 135-140 (1995)

Ozawa S. 等人：“肝脏中两种相关形式的芳基磺基转移酶的主要结构和特性。”

Sugiura Y.et al: "Extensive resection of the Bile ducts combined with liver resoction for cancer of the onaio hepatic duct junction" Surgery. 115. 445-51 (1994)

Sugiura Y.等人：“胆管广泛切除联合肝切除治疗onaio肝管交界处癌症”手术。