Genetical Instability induced by Low Doses of Radations

低剂量辐射引起的遗传不稳定性

• 批准号: 06454640
• 负责人: WATANABE Masami
• 金额: $ 4.67万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454640/
• 关键词: low dose radiations; human embryo cells; in vitro aging; chromosome aberration; aneuploidy; radiaton response; genetical instability

We have reported that chronic radiations with low doses of X-rays affected to several biological responses in human cells. For example, repeated-irradiation with 1 cGy per week of gamma-rays was significantly prolonged over that of control. Although non-irradiated control and acute irradiated cells at passage 26 (about 10 passages to senescence) were normal karyotype and number, chronic-irradiated cells at the same passage showed numerical, rather than structural, abnormalities. We also found that chromoshone aberration frequency of a cell irradiated with low doses of X-rays is high around 50 times of the unirradiated cells at 14 days after irradiation.These results can best be interpreted by assuming that ionizing radiation at low doses induces a stress response, probably an epigenetic one, which is responsible for the majority of the genetic instability. We speculate that non genetic materials, such as cell membrene and cell division apparatus may be important target of low dose radiations.

我们已经报道了低剂量X射线慢性辐射对人体细胞的几种生物反应的影响。例如，每周1 cGy的γ射线的重复照射比对照组显著延长。尽管第26代（约10代至衰老）的未辐照对照和急性辐照细胞的核型和数量正常，但同一代的慢性辐照细胞显示数量异常，而非结构异常。我们还发现，低剂量X射线照射后14天，细胞染色体畸变率约为未照射细胞的50倍，这一结果可以通过假设低剂量电离辐射诱导应激反应来解释，这可能是一种表观遗传反应，它是造成遗传不稳定性的主要原因。我们推测，非遗传物质，如细胞膜和细胞分裂器可能是低剂量辐射的重要靶点。

项目成果

Y.Kagawa, F.Yatagai, M.Suzuki, Y.Kase, A.Kobayashi, M.Hirano, T.Kato, M.Watanabe : and F.Hanaoka: "Analysis of mutations in the human hprt gene induced by accelerated heavy-ion irradiation" J.Radiat.Res.36. 185-195 (1995)

Y.Kakawa、F.Yatagai、M.Suzuki、Y.Kase、A.Kobayashi、M.Hirano、T.Kato、M.Watanabe：和 F.Hanaoka：“加速重离子诱导的人类 hprt 基因突变分析

K.Suzuki, and M.Watanabe: "Modulation of cell growth and mutation induction by introduction of the expression vector of human hsp7O gene" Exp.Cell Res.214. 75-81 (1994)

K.Suzuki 和 M.Watanabe：“通过引入人类 hsp7O 基因的表达载体来调节细胞生长和突变诱导”Exp.Cell Res.214。

M.Suzuki,M.Watanabe et al.: "LET dependence of cell death,mutation induction and chromatin damage in human cells irradiated with accelerated carbon ions" Adv.Space Biol.18. 127-136 (1995)

M.Suzuki,M.Watanabe 等人：“用加速碳离子照射的人类细胞中细胞死亡、突变诱导和染色质损伤的 LET 依赖性”Adv.Space Biol.18。

M.Watanabe: "Required effort to evolve toxicity testing using in vitro methods" Altem.Animal Test.Exp.3. 80-86 (1995)

M.Watanabe：“使用体外方法发展毒性测试所需的努力”Altem.Animal Test.Exp.3。

Sugahara,T.and Watanabe,M.: "Radiobiological Conceopts in Radiotherapy" Radiation paradigm in prespectives, 1-5 (1995)

Sugahara,T. 和 Watanabe,M.：“放射治疗中的放射生物学概念”放射范式展望，1-5 (1995)