RADIATION-INDUCED LONG LIVED RADICALS CAUSING MUTATION AND TRANSFORMATION
辐射诱导的长寿命自由基导致突变和转化
基本信息
- 批准号:13480171
- 负责人:
- 金额:$ 9.6万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Many researches studying in area of radiation biology have been believed that active short life-time radicals such as OH and H radicals, play an important role to express genoto do effects of radiation h cells, such as mutation and cancer induction. However, we found a new type of radicals with long life-time (T_<1/2>20hr) in gamma ray hmdiated golden hamster embryo (GHE) cells at room temperature by using ESR spectroscopy. It may be more important in mutation induction than the active short live radials. When Vitamin C (MA) is added to the gamma-or X-irradiated cells at 20 min or 6 hr after the irradiation, respectively, LLR are scavenged by them simuhaneousty with the drastic suppression of genotoxicity such as mutation and transformation. Since reactive oxygen species (ROS) disappear within microsecond after the irradiation, AsA do not scavenge the ROS but LLR. Therefore, we have proposed that LLR must be responsible radicals for inducing mutation, and probably important for the genotoxicity in the irradiated mammalian cells. Addition of AsA to the cells before or after irradiation does not have any effects on reducing cell death and chromosomal aberration. By the studies using ESEEM spectroscopy, we found that LLR probably locate in interior of biopolymers where few water molecules exist. By further analysis of the ESR spectra of LLR and comparison of the yields of radicals in proteins and DNA, we reached to the conclusion that LLR are produced in protein and assigned as sulfinyl radicals as oxidized cysteine groups. LLR are not produced in DNA or in lipids. Our result show that the LLR produced in protein is responsible for the gene mutation in irradiated cells. Although this contradicts now common view in the radiation biology, many unresolved phenomena in the radiation biology can be explained well.
许多辐射生物学领域的研究认为,活跃的短寿命自由基,如OH和H自由基,在表达辐射h细胞的基因效应,如突变和致癌方面发挥着重要作用。然而,我们用电子自旋共振波谱在室温下的金黄地鼠胚胎(GHE)细胞中发现了一种新的长寿命自由基(T_<;1/2>;20hr)。在突变诱导中,它可能比活跃的短寿命半径更重要。在照射后20分钟或6小时分别加入维生素C(MA),细胞可清除LLR,同时对突变、转化等遗传毒性也有明显的抑制作用。由于活性氧物种(ROS)在照射后的微秒内消失,AsA不清除ROS,而是清除LLR。因此,我们提出LLR可能是诱发突变的重要自由基,并且可能对辐射后哺乳动物细胞的遗传毒性起重要作用。照射前或照射后细胞中加入AsA对减少细胞死亡和染色体畸变率没有任何作用。通过ESEEM光谱的研究,我们发现LLR可能位于水分子较少的生物聚合物内部。通过进一步分析LLR的ESR谱,并比较蛋白质和DNA中自由基的产率,我们得出结论:LLR是在蛋白质中产生的,并以氧化半胱氨酸基的形式被归类为亚磺基。LLR不是在DNA或脂类中产生的。我们的结果表明,蛋白质中产生的LLR是辐射细胞中基因突变的原因。虽然这与目前辐射生物学中的普遍观点相矛盾,但辐射生物学中的许多未解决的现象都可以很好地解释。
项目成果
期刊论文数量(79)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hamada, N., Kodama, S., Suzuki, K., Watanabe, M.: "Gap junctional intercellular communication and cellular response to heat stress"Carcinogenesis. 24・11. 1723-1728 (2003)
Hamada, N.、Kodama, S.、Suzuki, K.、Watanabe, M.:“间隙连接细胞间通讯和细胞对热应激的反应”24・11(2003)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kumagai, J., Nakama, M., Miyazaki, T., Ise, T., Kodama, S., Watanabe, M.: "Scavenging of long-lived radicals by (-)-epigalloctechin-3-O-gallate and simultaneous suppression of mutation in irradiated mammalian cells."Radiat.Phys.Chem.. 64. 293-297 (2002)
Kumagai, J.、Nakama, M.、Miyazaki, T.、Ise, T.、Kodama, S.、Watanabe, M.:“通过 (-)-epigalloctechin-3-O-没食子酸酯清除长寿命自由基和
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Matsuda, N., Horikawa, M., Yoshida, M., Watanabe.M, Nagahata, M., Teramoto, A., Abe, K: "Enhanced DNA synthesis accompanied by constitutive phaophorylation of the ERK pathway in human fibroblasts cultured on a polyeectrolyte complex."Biomaterials. 24. 477
Matsuda, N.、Horikawa, M.、Yoshida, M.、Watanabe.M、Nagahata, M.、Teramoto, A.、Abe, K:“在培养的人成纤维细胞中,DNA 合成增强,伴随 ERK 途径的组成型磷酸化。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
M.Miyakoda, K.Suzuki, S.Kodama, M.Watanabe: "Activation of ATM by heat shock and phosphorylation of p53"Oncogene. 21・7. 1090-1096 (2002)
M.Miyakoda、K.Suzuki、S.Kodama、M.Watanabe:“通过热休克和 p53 磷酸化激活 ATM”Oncogene 21・7 (2002)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
N.Nakahata, M.Miyakoda, K.Suzuk i, S.Kodama, M.Watanabe: "Heat shock induces centrosomal dysfunction, and causes non-apoptotic catastrophe in human tumor cells"Intern. J. Hyper.. 18・4. 332-343 (2002)
N.Nakahata、M.Miyakoda、K.Suzuk i、S.Kodama、M.Watanabe:“热休克导致中心体功能障碍,并导致人类肿瘤细胞发生非凋亡灾难”实习生 J. Hyper.. 18・4。 332-343 (2002)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
WATANABE Masami其他文献
WATANABE Masami的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('WATANABE Masami', 18)}}的其他基金
Development of novel intravesical agents for the treatment of refractory superficial bladder cancer
开发治疗难治性浅表性膀胱癌的新型膀胱灌注药物
- 批准号:
22791473 - 财政年份:2010
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Cell transformation route and the developmental mechanics which assumed chromosomal aneuploidy an origin
以染色体非整倍性为起源的细胞转化途径和发育机制
- 批准号:
21310036 - 财政年份:2009
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Examination for detection of early glaucoma with ON- and OFF-delays of subjects with normal and glaucomatous eyes
对正常和青光眼受试者进行开和关延迟检测早期青光眼的检查
- 批准号:
19592051 - 财政年份:2007
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Radiation-induced long-lived radicals causing mutation and carcinogenesis
辐射诱导的长寿命自由基导致突变和致癌
- 批准号:
16101002 - 财政年份:2004
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Functional Recovery of Vision with Axonal Regeneration in Crushed Optic Nerve
受损视神经轴突再生的视觉功能恢复
- 批准号:
16591780 - 财政年份:2004
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
PROTECTION FROM DEATH OF AXOTOMIZED RETINAL GANGLION CELLS AND PROMOTION OF THEIR AXONAL REGENERATION
防止轴切视网膜神经节细胞死亡并促进其轴突再生
- 批准号:
12671733 - 财政年份:2000
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
AN IMPORTANT ROLE OF GENETICAL INSTABILITY IN RADIATION CARCINOGENESIS
遗传不稳定性在辐射致癌中的重要作用
- 批准号:
09480126 - 财政年份:1997
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Genetical Instability induced by Low Doses of Radations
低剂量辐射引起的遗传不稳定性
- 批准号:
06454640 - 财政年份:1994
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Research on Regeneration of the Optic Nerve and Functional Recovery of Retinal Ganglion Cells with Regenerated Axons
轴突再生对视神经再生及视网膜神经节细胞功能恢复的研究
- 批准号:
03670841 - 财政年份:1991
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似国自然基金
Vitamin C 通过诱导全长 GSDME 依赖的细胞焦亡激活抗肿瘤免疫治疗宫颈癌
- 批准号:
- 批准年份:2024
- 资助金额:20.0 万元
- 项目类别:省市级项目
Vitamin C通过GSMDC调控细胞焦亡的新机制
- 批准号:
- 批准年份:2022
- 资助金额:30 万元
- 项目类别:青年科学基金项目
去乙酰化酶SIRT1在大剂量Vitamin C治疗失血性休克内皮损伤的作用研究
- 批准号:81501643
- 批准年份:2015
- 资助金额:18.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Impact of Vitamin C on the Reduction of Opioid Consumption After an Emergency Department Visit for Acute Musculoskeletal Pain: A Double-Blind Randomized Control Trial
维生素 C 对急性肌肉骨骼疼痛急诊科就诊后减少阿片类药物消耗的影响:双盲随机对照试验
- 批准号:
479465 - 财政年份:2023
- 资助金额:
$ 9.6万 - 项目类别:
Operating Grants
Role of Vitamin C in Low back Pain and Intervertebral Disc Degeneration
维生素 C 在腰痛和椎间盘退变中的作用
- 批准号:
10741198 - 财政年份:2023
- 资助金额:
$ 9.6万 - 项目类别:
Elucidation of the molecular mechanism of protective action by vitamin C against septic shock
阐明维生素 C 对抗感染性休克的保护作用的分子机制
- 批准号:
23K06410 - 财政年份:2023
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Vitamin C to Decrease Effects of Smoking during Pregnancy on Offspring Airway Function, Airway Size, and Epigenetic Correlates: VCSIP cohort follow-up through 10 Years of Age
维生素 C 可减少怀孕期间吸烟对后代气道功能、气道大小和表观遗传相关性的影响:VCSIP 队列随访至 10 岁
- 批准号:
10658356 - 财政年份:2023
- 资助金额:
$ 9.6万 - 项目类别:
Perioperative vitamin C to reduce persistent pain after total knee arthroplasty: a pilot randomized controlled trial
围手术期维生素 C 可减轻全膝关节置换术后持续性疼痛:一项随机对照试验
- 批准号:
473049 - 财政年份:2022
- 资助金额:
$ 9.6万 - 项目类别:
Operating Grants
Effect of vitamin C on neural development of the cerebral cortex.
维生素C对大脑皮层神经发育的影响。
- 批准号:
22K11775 - 财政年份:2022
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Vitamin C research based on vitamin C transporters
基于维生素C转运蛋白的维生素C研究
- 批准号:
22K19369 - 财政年份:2022
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Characterization of a novel solute carrier in the Vitamin C transport pathway
维生素 C 运输途径中新型溶质载体的表征
- 批准号:
550959-2020 - 财政年份:2020
- 资助金额:
$ 9.6万 - 项目类别:
University Undergraduate Student Research Awards
Mechanisms of improved organ function following sepsis treatment with vitamin c, thiamine and hydrocortisone (triple therapy)
维生素 C、硫胺素和氢化可的松治疗败血症后改善器官功能的机制(三联疗法)
- 批准号:
9978302 - 财政年份:2020
- 资助金额:
$ 9.6万 - 项目类别:
Vitamin C-induzierte DNA-Demethylierung als Präventivtherapie für Leukämie
维生素 C 诱导的 DNA 去甲基化作为白血病的预防疗法
- 批准号:
446251518 - 财政年份:2020
- 资助金额:
$ 9.6万 - 项目类别:
WBP Fellowship