Cell transformation route and the developmental mechanics which assumed chromosomal aneuploidy an origin

以染色体非整倍性为起源的细胞转化途径和发育机制

• 批准号: 21310036
• 负责人: WATANABE Masami
• 金额: $ 12.15万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21310036/
• 关键词: 染色体異数化; 中心体; 細胞がん化; ミトコンドリア機能; 自然発がん; メロテリック結合; 長寿命ラジカル; 微小核細胞融合法; 擬二極分裂; 染色体取り残し; 放射線発がん; 三倍体; 四倍体; 遺伝子発現

It has been believed that the first target of radiation carcinogenesis is DNA. However, this is not proved for radiation carcinogenesis yet. We discovered that frequency of aneuploid cell was closely related to that of radiation-induced cell transformation, but gene mutation was not. Aneuploidy was seen in high frequency in early process of cell transformation. These results strongly suggest that a main target of carcinogenesis by low dose radiation is not DNA, but is centrosome, which are the proteins to constitute chromosomal homeostasis maintenance mechanism.

人们一直认为，辐射致癌的第一个目标是DNA。然而，这还没有被证明是辐射致癌的。我们发现非整倍体细胞的频率与辐射诱导细胞转化的频率密切相关，但基因突变不相关。非整倍体在细胞转化早期出现频率较高。这些结果强烈提示低剂量辐射致癌的主要靶点不是DNA，而是中心体，中心体是构成染色体稳态维持机制的蛋白质。

项目成果

The Contribution of Radiation-induced Large Deletion of the Genome to Chromosomal Instability

• DOI: 10.1667/rr1464.1
• 发表时间: 2009-02-01
• 期刊: RADIATION RESEARCH
• 影响因子: 3.4
• 作者: Toyokuni, Hideaki;Maruo, Atsushi;Watanabe, Masami
• 通讯作者: Watanabe, Masami

Biological effects of low dose radiation, A Symposium on"UrgentSymposium for The accidents in Fukushima Daiichi nuclear power plant"

低剂量辐射的生物效应，“福岛第一核电站事故紧急研讨会”研讨会

• 发表时间: 2011
• 作者: K. Ono;S. Endo;K. Tanaka;M. Hoshi;Y. Hirokawa;櫻井次郎;Watanabe M
• 通讯作者: Watanabe M

SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA

• DOI: 10.4061/2010/795946
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF NUCLEIC ACIDS
• 影响因子: 2.3
• 作者: Inoue, Eri;Tano, Keizo;Enomoto, Takemi
• 通讯作者: Enomoto, Takemi

Micronuclei formation induced by X-irradiation does not always result from DNA double-strand breaks

X 辐射诱导的微核形成并不总是由 DNA 双链断裂引起

• 发表时间: 2012
• 期刊: J Radiat Res(Tokyo)
• 作者: Okada T;Kashino G;Nishiura H;TanoK;Watanabe M.
• 通讯作者: Watanabe M.

放射線発がんへのミトコンドリアの関与、ワークショップ『放射線生物学と活性酸素(酸化ストレス)-ミトコンドリアの役割-』

线粒体参与辐射致癌，研讨会“放射生物学和活性氧（氧化应激）-线粒体的作用”

• 发表时间: 2011
• 作者: 渡邉正己;菓子野元郎;熊谷純;渡邉喜美子;田野恵三
• 通讯作者: 田野恵三