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8番染色体短腕上の大腸癌の進展を抑制する遺伝子の検索

在 8 号染色体短臂上寻找抑制结直肠癌进展的基因

基本信息

批准号：
07670266
负责人：
田中貴代子
金额：
$ 1.41万
依托单位：
Tokyo Metropolitan Organization for Medical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至无数据
项目状态：
已结题

项目摘要

大腸癌の発生、進展には複数の遺伝子変化が起こっていることが明らかになってきているが、転移、浸潤に関与する遺伝子変化については現在のところまだ判っていない。既に我々は、転移性を示す進行大腸癌で8番染色体短腕の欠失を高頻度に認めている。また、正常8番染色体移入大腸癌細胞では癌形質の抑制と、浸潤能の低下が起こるが、移入細胞から8番染色体特定領域が脱落した細胞(リバータント細胞)では癌形質が再発現し、浸潤能も元の大腸癌細胞と同程度になったことからも、8番染色体上に大腸癌の進展に関与する癌抑制遺伝子の存在が示唆された。そこで、正常8番染色体特定領域を含む染色体DNAから制限酵素認識配列を含むプライマーを用いて、塩基配列情報未知の染色体DNAの全配列を増幅した。増幅したDNAを制限酵素で切断し、ブルースクリプトIIにクローニングしてDNAライブラリーを作製し、ラベルしたヒト全ゲノムDNAをプローブとしてライブラリーをスクリーニングして単一コピークローンを得た。さらに正常8番染色体移入大腸癌細胞とリバータント細胞のcDNAをプローブとしてハイブリダイゼイションを行い、正常8番染色体移入大腸癌細胞でのみ発現しているゲノムDNAを数クローンを得た。現在、得られたクローンをプローブとしてノーザン分析により正常8番染色体移入大腸癌細胞とリバータント細胞でmRNAの発現を確かめている。

In the case of cancer, the number of people who are in the process of developing cancer is very important. Do not know how to treat cancer, transfer, immersion, and chemotherapy. Now, you can determine whether you are going to be affected. Both we and transfer tests show that there are 8 times chromosome short wrist loss and high degree of chromosome loss in breast cancer. The transfer of normal and normal chromosomes into large cancer cells, the inhibition of carcinomatous form, the ability of immersion to lower the number of chromosomes, the specific field of chromosome exfoliation, the number of exfoliated cells, the reappearance of cancer, the ability of immersion, the same degree of cancer cells. On the chromosome 8, there was an instigation of cancer progression and cancer suppressor gene on the chromosome. In the specific field of chromosome DNA and normal chromosome, the specific field contains chromosome DNA, the restriction enzyme, the full alignment of chromosomal DNA and the amplitude of chromosomes. The enzyme was cut off by DNA, the enzyme was cut off, the enzyme was cut, the DNA was cut, the enzyme was cut, and the enzyme was cut. The normal chromosome 8 was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the normal chromosome was transferred into the large cancer cell, the Now, we can tell you that the normal chromosomes have been transferred into large cancer cells by mRNA analysis.