Nuclear Magnetic Resonance Analysis in Urological Disorders.

泌尿系统疾病的核磁共振分析。

• 批准号: 59480324
• 负责人: KATO Tetsuro
• 金额: $ 3.9万
• 依托单位: Akita University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480324/
• 关键词: Nuclear Magnetic Resonance; Water Content; Relaxation Time; 膀胱腫瘍

1.Preservation and measuring condition of blood,urine and tissue samples: NMR relaxation times(T1 and T2) of tissues which were kept frozen by dry-ice remained almost stable within 24 hours after sampling. It was important to eliminate serum protein using trichloroacetic acid or a filter, because serum protein was an obstacle to judge a peak of lactate in NMR spectroscopic analysis of serum.2.T1 and T2 through VX2 carcinoma growth: T1 values of tumor tissue gradually increased along with tumor growth, being greater than the control values which remained constant. T1 values of the non-tumorous bladder tissue were lower than those of the tumor throughout the middle stage of tumor growth(4 week), and then showed prominent increase at the end stage of tumor growth(5-6 weeks).T2 value of the tumor tissue did not show any characteristic changes as compared with the controls throughout the progress of the tumor. T2 values of the non-tumorous bladder tissue also presented no peculiar change until the middle stage, but again showed a rapid increase as obseved in T1 monitoring, in the end stage.T1 and T2 values in both the non-tumorous tisse and the control bladder tissue well correlated with the tissue water content throughout the tumor growth, while these on the tumor tissue represented no significant correlation with water content as a whole. However, the relaxation times in the tumor tissue well correlated with water content in the individual tumor stage,suggesting that relaxation times in tumor is influenced not only by water content but also by water-macrolmolecular interaction.

1.血液、尿液和组织样本的保存和测量条件：用干冰冷冻保存的组织的NMR弛豫时间（T1和T2）在采样后24小时内基本保持稳定。重要的是使用三氯乙酸或过滤器去除血清蛋白，因为血清蛋白是判断血清NMR光谱分析中乳酸峰的障碍。2.通过VX 2癌生长的T1和T2：肿瘤组织的T1值随着肿瘤生长沿着逐渐增加，大于保持恒定的对照值。非肿瘤性膀胱组织的T1值在肿瘤生长中期（4周）低于肿瘤组织，然后在肿瘤生长末期（5-6周）出现明显升高;肿瘤组织的T2值在整个肿瘤进展过程中与对照组相比没有表现出任何特征性变化。非肿瘤性膀胱组织的T2值在中期也无特殊变化，但在T1监测中，在末期又显示出快速增加。非肿瘤性膀胱组织和对照膀胱组织的T1和T2值与整个肿瘤生长过程中的组织含水量有很好的相关性。而肿瘤组织上的这些与总体上的含水量没有显著相关性。然而，在肿瘤组织中的弛豫时间以及相关的水含量在个别肿瘤阶段，这表明在肿瘤弛豫时间的影响，不仅由水含量，但也由水-大分子相互作用。

项目成果

加藤哲郎: 日本癌学会総会記事. 41. 347 (1982)

加藤哲郎：日本癌症协会大会文章 41. 347 (1982)

Masahiro Nishimura; Toshio Suzuki; Etsuko Sato et al: Proceeding of The Japanese Cancer Association The 41th Annual Meeting. 41. 347 (1982)

西村正宏；

Kazunari Sato; Masatsugu Moriyama; Ryoetsu Abe et al: "H Nuclear Magnetic Resonance Relaxation Times Through VX2 Carcinoma Growth in Rabbit Bladder" Japan Journal of Urology. 76. 1161-1170 (1985)

佐藤一成；

西村政博: 日本癌学会総会記事. 41. 347 (1982)

Masahiro Nishimura：日本癌症协会大会文章 41. 347 (1982)。

佐藤一成: 日本泌尿器科学会誌. 76. 1161-1170 (1985)

佐藤一成：日本泌尿外科协会杂志 76. 1161-1170 (1985)。