Nuclear Magnetic Resonance Analysis in Urological Disorders.

1.血液、尿液、组织样品保存及测量条件：干冰冷冻后组织的核磁共振弛豫时间（T1、T2）在取样后24小时内基本保持稳定。血清蛋白在血清核磁共振分析中是判断乳酸峰的障碍，因此用三氯乙酸或过滤器去除血清蛋白是很重要的。T1、T2至VX2肿瘤生长：肿瘤组织T1值随肿瘤生长逐渐升高，大于对照组，对照组保持不变。非肿瘤膀胱组织T1值在肿瘤生长中期（4周）低于肿瘤组织T1值，在肿瘤生长末期（5-6周）T1值显著升高。与对照组相比，肿瘤组织的T2值在整个肿瘤发展过程中未表现出任何特征性变化。非肿瘤膀胱组织的T2值直到中期也未出现特殊变化，但在终末期T1监测中再次出现快速升高。在整个肿瘤生长过程中，非肿瘤组织和对照膀胱组织的T1和T2值与组织含水量呈良好的相关性，而肿瘤组织的T1和T2值与含水量总体上无显著相关性。然而，肿瘤组织的弛豫时间与单个肿瘤阶段的含水量有很好的相关性，这表明肿瘤组织的弛豫时间不仅受含水量的影响，还受水-大分子相互作用的影响。

1.Preservation and measuring condition of blood,urine and tissue samples: NMR relaxation times(T1 and T2) of tissues which were kept frozen by dry-ice remained almost stable within 24 hours after sampling. It was important to eliminate serum protein using trichloroacetic acid or a filter, because serum protein was an obstacle to judge a peak of lactate in NMR spectroscopic analysis of serum.2.T1 and T2 through VX2 carcinoma growth: T1 values of tumor tissue gradually increased along with tumor growth, being greater than the control values which remained constant. T1 values of the non-tumorous bladder tissue were lower than those of the tumor throughout the middle stage of tumor growth(4 week), and then showed prominent increase at the end stage of tumor growth(5-6 weeks).T2 value of the tumor tissue did not show any characteristic changes as compared with the controls throughout the progress of the tumor. T2 values of the non-tumorous bladder tissue also presented no peculiar change until the middle stage, but again showed a rapid increase as obseved in T1 monitoring, in the end stage.T1 and T2 values in both the non-tumorous tisse and the control bladder tissue well correlated with the tissue water content throughout the tumor growth, while these on the tumor tissue represented no significant correlation with water content as a whole. However, the relaxation times in the tumor tissue well correlated with water content in the individual tumor stage,suggesting that relaxation times in tumor is influenced not only by water content but also by water-macrolmolecular interaction.