Development of Antibodies to Angiogenic Factors for Diagnosis and Therapy of Solid Tumors.

tumor angiogenesis and angiogenic factor was evaluated in renal cell carcinoma （RCC） and urinary bladder carcinoma （BC-D2a-D2）的关系。VEGF genes， VEGF基因D2121基因D2 and VEGF基因D2165基因D2， were overexpressed in 60%of RCCs. VEGF基因的表达水平与体内微血管密度有明显的相关性。一项免疫病理学分析发现，一项与抗VEGF抗VEGF抗血清素一起被免疫性地提高到VEGF的N-末端的兔子。细胞系的细胞系出现在VEGF抑制中是积极的。VEGF基因在40%的BCAS中也被过度表达。在蛋白质水平上， VEGF在血癌细胞中明显阳性。VEGF-受体基因在两个肿瘤中表达了与VEGF基因表达水平的一致性。RCC和Bca中的血管生成症建议由VEGF及其受体的副作用机制建立。VEGF相关基因（VEGF-A、B、C和D）及其受体基因存在。 ... More 另一个克隆了一个。我们研究了这些基因在RCC中的表达，并发现了VEGF-A和受体、VEGFR-1和VEGFR-2，在VEGF-B和VEGF-C很少的情况下，在RCC的血管生成中发挥了关键作用。根据以上结果，我们比较了RCC患者组和健康控制组之间的血清VEGF水平。血清中VEGF的平均水平在RCC患者组中高于健康控制组。后操作血清VEGF水平明显地减少为与预先操作水平相比。血清VEGF水平与肿瘤体积、metastasis和阶段有显著的联系。当VEGF截断值设置为100 pg/ml时，根据接收器操作员曲线分析、敏感性和RCC的特性分别为80.0%和72.5%，适当地。Thus we suggest that RCC releases VEGF into the blood circulation and serum VEGF level would be a marker of malignant potential of RCC.Taken together， these results would be useful as basic data for the development of anti-angiogenic-factor antibody facilitating diagnosis and therapy of RCC。Less（低）

The relationship between tumor angiogenesis and angiogenic factors was evaluated in renal cell carcinoma (RCC) and urinary bladder carcinoma (BCィイD2aィエD2). VEGF genes, VEGFィイD2121ィエD2 and VEGFィイD2165ィエD2, were overexpressed in 60% of RCCs. The Expression level of VEGF genes was significantly correlated with the intratumoral microvessel density. An immunohistologic analysis was done with and anti-VEGF antiserum that had been raised by immunizing a rabbit with an oligopeptide homologous to the N-terminal of VEGF. Cytoplasm of RCC appeared to be positive to the VEGF staining. VEGF genes were also overexpressed in 40% of Bcas. At protein level, VEGF was apparently positive in bladder carcinoma cells. VEGF-receptor genes were expressed in concordance with the VEGF gene expression level in both tumors. Thus, angiogenesis in RCC and Bca is suggested to be facilitated by a paracrine mechanism with VEGF and its receptors.VEGF-relating genes (VEGF-A,B,C, and D) and their receptor genes have been … More cloned one after another. We studies the expression of these genes in RCCs and found that VEGF-A and the receptors, VEGFR-1 and VEGFR-2, play crucial roles in the angiogenesis of RCC while VEGF-B and VEGF-C do little.Based on the above results, we compared serum VEGF levels between a RCC patient group and a healthy control group. The mean serum VEGF level was higher in the RCC patient group than in the healthy control group. Postoperative serum VEGF level apparently decreased as compared with the preoperative level. Serum VEGF level was significantly correlated with tumor volume, metastasis, and stage. When the VEGF cut-off value was set at 100pg/ml based on a receiver-operator curve analysis, the sensitivity and the specificity of RCC were 80.0% and 72.5%, respectively. Thus we suggest that RCC releases VEGF into the blood circulation and serum VEGF level would be a marker of malignant potential of RCC.Taken together, these results would be useful as basic data for the development of anti-angiogenic-factor antibody facilitating diagnosis and therapy of RCC. Less