Comprehensive study to the evaluation of the host sensitivity and treatment effects following periodontal therapy

牙周治疗后宿主敏感性及治疗效果评价的综合研究

• 批准号: 60480409
• 负责人: ISHIKAWA Isao
• 金额: $ 3.78万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480409/
• 关键词: Initial preparation; Micro-ELISA technique; Serum IgG antibody titers; Photographic subtraction radiography; Neutrophil chemotaxis; Spirochetes; Subgingival plaque; 生体反応の多様性

For the purpose of evaluating the host sensitivity and treatment effects after periodontal therapy, the following studies were performed:1. The minute changes of alveolar bone was estimated using photographic subtraction radiography before and after the initial preparation. This mehtod revealed that the improvement or destructed alveolar bone was accelerated by the rapid resolution of severe inflammation of gingiva.2. New method "Perio Test" was used for the clinical trial to measure the tooth mobility.3. Neutrophil chemotaxis of Down's syndrome patients was measured for the purpose of investigation the relationship between the severity of the periodontal disease and the defective neutrophil chemotaxis. These results showed that the neutrophil chemotaxis in the Down's syndorome patients was significantly impaired when compared with the healthy volunteers and that the Down's syndrome patients with severe bone loss showed defective chemotaxis.4. A comparehensive culture methods of subgin … More gival microflora with a spirochetes was investigated. E G agar plate using steel-wool method was shown the excellent result for the isolation of subgingival microorganisms. For the cultivation of abligate anaerobic spirochetes, medium 10 supplemented with 10% rumen fluid by plate-inbottle method was obtained the best result.5. Serum IgG antibody titers against sonicated antigens of seven periodontopathic bacteria were determined by a micro-ELISA technique in the patients with periodontal disease.(1) The antibody titer against B. gingivalis was significantly correlated to the degree of periodontal destruction and decreased after periodontal treatment.(2) Most patients with advanced periodontitis (94.5%) exhibited high IgG titers against various periodontopathic bacteria.These serum always included high IgG titer against each or both of B. gingivalis and A. actinomycetemcomitans. 5.5% of patients showed lower IgG titer than healthy subjects and then PMNs also showed dysfunction of chemotaxis.These results suggested one must notice the diversity of host responses in the patients with advanced periodontitis. Less

为了评价牙周治疗后宿主的敏感性和治疗效果，本研究进行了以下研究：1.采用摄影减影技术评价牙周治疗前后牙槽骨的细微变化。本实验结果表明，牙周炎的快速消退加速了牙槽骨的改善或破坏。临床试验采用“Perio试验”新方法测量牙齿活动性。通过对唐氏综合征患者中性粒细胞趋化功能的测定，探讨牙周病的严重程度与中性粒细胞趋化功能缺陷的关系。这些结果表明，与健康志愿者相比，唐氏综合征患者的中性粒细胞趋化功能明显受损，伴有严重骨丢失的唐氏综合征患者的中性粒细胞趋化功能存在缺陷。枯草杆菌(Subgin…)高效培养方法的比较调查了更多含有螺旋体的Gival微生物区系。采用钢毛法的E-G琼脂平板分离龈下微生物效果较好。用平板瓶装法添加10%瘤胃液培养灭活厌氧螺旋体的效果最好。用微量ELISA法测定了牙周病患者血清中抗7种牙周病细菌超声抗原的免疫球蛋白G抗体效价，结果表明：(1)牙周炎患者血清中抗牙周炎杆菌抗体滴度与牙周破坏程度密切相关，经牙周治疗后呈下降趋势，晚期牙周炎患者(94.5%)对多种牙周病细菌均有较高的抗体滴度，其中牙周炎杆菌和伴生放线菌均有较高的抗体滴度。5.5%的牙周炎患者血清免疫球蛋白抗体滴度低于正常人，中性粒细胞也出现趋化功能障碍，提示晚期牙周炎患者应注意宿主反应的多样性。较少

项目成果

Y. IZUMI: "Neutrophil Chemotaxis of Down's Syndrome Patients in Relation to Periodontal Conditions" J. Stomatol. Soc., Jpn.53. 689-698 (1986)

Y. IZUMI：“唐氏综合症患者的中性粒细胞趋化性与牙周病的关系”J. Stomatol。

和泉雄一: 口腔病学会雑誌. 53. 689-698 (1986)

Yuichi Izumi：口腔医学会杂志 53. 689-698 (1986)。

M. UMEDA: "An Improved Method for Comprehensive Investigation of Subgingival Microflora with a Particular Reference to Obbigate Anaerobic Spirochetes" J. Japan Ass. Periodont.29. 91-105 (1987)

M. UMEDA：“一种改进的龈下微生物区系综合调查方法，特别是针对 Obbigate 厌氧螺旋体”，J. Japan Ass。

梅田誠: 日本歯周病学会会誌. 29. 91-105 (1987)

Makoto Umeda：日本牙周病学会杂志 29. 91-105 (1987)。

T. MERA: "Alveolar Bone Changes after Initial Preparation Observed with Subtraction Radiography" J. Japan. Ass. Periodont.27. 902-911 (1985)

T. MERA：“用减影射线照相观察初步准备后牙槽骨的变化”J. Japan。