Comprehensive study to the evaluation of the host sensitivity and treatment effects following periodontal therapy

为了评估牙周治疗后宿主的敏感性和治疗效果，进行了以下研究：初步预备前后，采用减影x线摄影法估计牙槽骨的微小变化。结果表明，严重的牙龈炎症的迅速缓解，加速了牙槽骨的改善或破坏。临床试验采用新方法“牙周试验法”测量牙齿的活动度。测定唐氏综合征患者中性粒细胞趋化性，探讨牙周病严重程度与中性粒细胞趋化性缺陷的关系。这些结果表明，唐氏综合征患者的中性粒细胞趋化性与健康志愿者相比明显受损，严重骨质流失的唐氏综合征患者的趋化性存在缺陷。研究了一种以螺旋体为主要微生物菌群的综合培养方法。结果表明，采用钢丝棉法的琼脂平板对龈下微生物有较好的分离效果。对于专性厌氧螺旋体的培养，瓶中平板法以添加10%瘤胃液的培养基10培养效果最佳。采用微量酶联免疫吸附试验（elisa）测定了牙周病患者血清中抗7种牙周病细菌超声抗原的IgG抗体滴度。(1)抗牙龈芽胞杆菌抗体滴度与牙周破坏程度显著相关，在牙周治疗后呈下降趋势。(2)大多数晚期牙周炎患者（94.5%）对各种牙周病细菌具有较高的IgG滴度。这些血清对牙龈白僵菌和放线菌均有较高的IgG滴度。5.5%的患者IgG滴度低于健康人，PMNs也出现趋化功能障碍。这些结果表明，必须注意宿主反应的多样性，在患者的晚期牙周炎。少

For the purpose of evaluating the host sensitivity and treatment effects after periodontal therapy, the following studies were performed:1. The minute changes of alveolar bone was estimated using photographic subtraction radiography before and after the initial preparation. This mehtod revealed that the improvement or destructed alveolar bone was accelerated by the rapid resolution of severe inflammation of gingiva.2. New method "Perio Test" was used for the clinical trial to measure the tooth mobility.3. Neutrophil chemotaxis of Down's syndrome patients was measured for the purpose of investigation the relationship between the severity of the periodontal disease and the defective neutrophil chemotaxis. These results showed that the neutrophil chemotaxis in the Down's syndorome patients was significantly impaired when compared with the healthy volunteers and that the Down's syndrome patients with severe bone loss showed defective chemotaxis.4. A comparehensive culture methods of subgin … More gival microflora with a spirochetes was investigated. E G agar plate using steel-wool method was shown the excellent result for the isolation of subgingival microorganisms. For the cultivation of abligate anaerobic spirochetes, medium 10 supplemented with 10% rumen fluid by plate-inbottle method was obtained the best result.5. Serum IgG antibody titers against sonicated antigens of seven periodontopathic bacteria were determined by a micro-ELISA technique in the patients with periodontal disease.(1) The antibody titer against B. gingivalis was significantly correlated to the degree of periodontal destruction and decreased after periodontal treatment.(2) Most patients with advanced periodontitis (94.5%) exhibited high IgG titers against various periodontopathic bacteria.These serum always included high IgG titer against each or both of B. gingivalis and A. actinomycetemcomitans. 5.5% of patients showed lower IgG titer than healthy subjects and then PMNs also showed dysfunction of chemotaxis.These results suggested one must notice the diversity of host responses in the patients with advanced periodontitis. Less