Molecular analysis of the interaction between immune function and bone metabolisms in periodontitis

牙周炎免疫功能与骨代谢相互作用的分子分析

• 批准号: 13470459
• 负责人: ISHIKAWA Isao
• 金额: $ 8.51万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470459/
• 关键词: periodontitis; RANKL; OPG; IL-1; T-cell; プロスタグランジン; 破骨細胞; 歯肉線維芽細胞; プロスタグランジンE2; ICAM-1

Periodontopathic bacteria, immune responses and bone metabolisms were examined to clarify the interaction between immune responses and bone metabolisms in periodontitis. We have established the detection system for periodontopathic bacteria using real-time PCR analysis. Based on the system, we have found that periodontopathic bacteria were frequently detected in the surgically removed vessels from the cardio-vascular disease patients with periodontitis. In addition, monocytes from periodontitis patients were activated compared with healthy subjects. Serum IL-6 concentration was significantly elevated immediately after periodontal treatment. These results suggested that periodontitis might affect systemic health through bacteremia or production of inflammatory cytokines by activated monocytes. Bone metabolisms are regulated by osteoclasts and osteoblasts, and osteoclast differentiation is regulated by RANKL and OPG produced by osteoblasts. We have reported that RANKL expression was high in severe periodontitis lesion, and T-cells from periodontitis tissue expressed RANKL. Both periodontal ligament fibroblasts (PDL) and gingival fibroblasts produced OPG. IL-1 augmented RANKL expression in PDL five times higher than HGF, but OPG production was lower than HGF.

通过对牙周病原菌、免疫反应和骨代谢的检测，阐明牙周炎免疫反应和骨代谢之间的相互作用。建立了牙周病细菌的实时定量聚合酶链式反应检测体系。基于该系统，我们发现在心血管疾病合并牙周炎患者手术切除的血管中经常检测到牙周病原菌。此外，牙周炎患者的单核细胞比健康人的单核细胞活化。牙周治疗后即刻血清IL-6浓度明显升高。提示牙周炎可能通过菌血症或激活的单核细胞产生炎性细胞因子而影响全身健康。骨代谢受破骨细胞和成骨细胞的调节，破骨细胞的分化受成骨细胞产生的RANKL和OPG调节。我们曾报道，RANKL在重度牙周炎病变中高表达，牙周炎组织中的T细胞表达RANKL。牙周膜成纤维细胞(PDL)和牙龈成纤维细胞均产生OPG。IL-1促进PDL RANKL的表达，是HGF的5倍，但OPG的产生低于HGF。

项目成果

2003 Consensus report, workshop on contemporary science in clinical periodontics.

2003 年临床牙周病当代科学研讨会共识报告。

• 发表时间: 2003
• 期刊: Annals of Periodontology 8・1
• 作者: Ishikawa I et al.

Lasers in Dentistry-Revolution of Dental Treatment in the New Millennium : Proceedings of the 8th International Congress on Lasers in Dentistry.

牙科中的激光——新千年牙科治疗的革命：第八届国际牙科激光大会论文集。

• 发表时间: 2003
• 作者: Ishikawa I;Frame JW;Aoki A (Eds.)
• 通讯作者: Aoki A (Eds.)

Iwasaki K, Noguchi K, Ishikawa I.: "Prostaglandin E2 downregulates interleukin-12 production through EP4 receptors in human monocytes stimulated with lipopolysaccharide from Actinobacillus."Oral Microbiol Immunol. 18. 150-155 (2003)

Iwasaki K、Noguchi K、Ishikawa I.：“前列腺素 E2 通过放线杆菌脂多糖刺激的人单核细胞中的 EP4 受体下调白细胞介素 12 的产生。”口腔微生物免疫学。

Expression of CD14, CD16 and CD45RA on monocytes from periodontitis patients

• DOI: 10.1111/j.1600-0765.2004.00713.x
• 发表时间: 2004-02-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Nagasawa, T;Kobayashi, H;Izumi, Y
• 通讯作者: Izumi, Y

LPS-stimulated human gingival fibroblasts inhibit the differentiation of monocytes into osteoclasts through the production of osteoprotegerin

• DOI: 10.1046/j.1365-2249.2002.01990.x
• 发表时间: 2002-11-01
• 期刊: CLINICAL AND EXPERIMENTAL IMMUNOLOGY
• 影响因子: 4.6
• 作者: Nagasawa, T;Kobayashi, H;Ishikawa, I
• 通讯作者: Ishikawa, I