The immunological investigations concerning the pathological mechanisms of periodontal diseases

我们关于牙周病的微生物和免疫机制的分析取得了许多富有成效的成果。结果如下：1）采用酶联免疫吸附试验（ELISA）检测了重度牙周炎患者血清中7种牙周致病菌的IgG抗体，结果表明B在牙周炎中的病理作用。gingivalis和A.伴放线菌在牙周病中的作用。2）我们的研究证实了唐氏综合征（DS）中确定性中性粒细胞趋化的程度及其与牙周病进展的关系。3）血小板活化因子（PAF），在发炎的人牙龈组织中检测到一种生物活性脂质，其与牙周炎的发生和持续直接相关。通过测定重度牙周炎患者外周血多形核白细胞（PMLs）的15-HETE，观察15-脂氧合酶活性。少年和快速亲 关于我们 退行性牙周炎组报告15-HETE合成低于年龄匹配的对照组。5）牙龈B菌W50、Veionella purvula ATCC 17745和A.伴放线菌ATCC 9710，和来自B的超声处理的全细胞提取物。gingivalis 381中的特异性抗原，用免疫印迹技术分析了不同牙周病患者和对照组的血清，记录了几条与牙周炎患者血清反应更频繁的主要条带。6）从晚期破坏性牙周炎（ADP）患者血清中筛选抗原反应性基因，构建伴放线菌Y 4噬菌体文库。这项研究表明，一个独特的DNA序列编码A. Y_4抗原与ADP患者血清呈强反应。因此，可作为牙周炎诊断的DNA探针。少

Our analysis concerning the microbiological and immunological mechanisms of periodontal diseases yielded many fruitful results. These results are as follows.1) Serum IgG antibodies of severe periodontitis patients were measured to seven periodontopathic bacteria with enzyme-linked immunosorbent assay(ELISA).The findings demonstrated the pathological role of B. gingivalis and A. actino mycetemcomitans played in periodontal disease.2) The degree of definitive neutrophil chemotaxis in Down' s syndrome(DS) and its relationship to periodontal disease progression was confirmed by our studies.3) Platelet-activating factor(PAF), a biological active lipid was detected in inflamed human gingival tissue directly associated with the occurrence and persistence of periodontal inflammation.4) Synthesis competence of 15-hydroxy-eicosatetraenoic acid(15-HETE) by polymorphonuclear leukocytes(PMLs) of severe periodontitis patients was measured to observe 15-lipoxygenase activity. Juvenile and rapidly pro … More gressive periodontitis groups reported lower 15-HETE synthesis than the age-matched controls. The results are relevant to those communications concerning juvenile and rapidly progressive periodontitis with abnormal PMNL function.5) Sonicated whole cell extracts and outer membrane proteins from B.gingivalis W50, Veionella purvula ATCC 17745 and A. actinomycetemcomitans ATCC 9710, and sonicated whole cell extract from B. gingivalis 381 were analyzed by the immunoblot technique using sera of patients with diverse periodontal diseases and control subjects.Several major bands to which the specific antigens reacted more frequently with sera from periodontitis patients were recorded. Monitoring of antibody response to specific antigens may be significant to assess human periodontal disease progress.6) Clones of genes with antigen reactive from sera of advanced destructive periodontitis(ADP) patients to construct a phage library of A.actinomycetemcomitans Y4 DNA were screened by the immunochemical analysis. This investigation indicated a distinctive DNA sequence encoding an A.a. Y4 antigen strongly reactive to the sera of the ADP patients. Hence, serve as a DNA probe in diagnosis of periodontitis. Less