The immunological investigations concerning the pathological mechanisms of periodontal diseases

我们对牙周病的微生物学和免疫学机制的分析取得了许多丰硕的成果。这些结果如下。1)采用酶联免疫吸附法（ELISA）检测重度牙周炎患者血清中7种牙周病细菌的IgG抗体。本研究结果证实了牙周病中牙龈芽胞杆菌和活动芽胞杆菌的病理作用。2)我们的研究证实了唐氏综合征（DS）中性粒细胞趋化程度及其与牙周病进展的关系。3)血小板活化因子（PAF）是一种生物活性脂质，与牙周炎症的发生和持续直接相关。4)测定重度牙周炎患者多形核白细胞（pml）合成15-羟基二十碳四烯酸（15-HETE）的能力，观察15-脂氧合酶活性。更严重的牙周炎组报告的15-HETE合成低于年龄匹配的对照组。这些结果与青少年和快速进行性牙周炎伴PMNL功能异常的交流有关。5)利用不同牙周病患者血清和对照组血清，采用免疫印迹技术对牙龈B.牙龈菌W50、紫癜Veionella ATCC 17745和放线菌ATCC 9710及牙龈B.牙龈菌381超声全细胞提取物的超声全细胞提取物和外膜蛋白进行分析。记录了几个主要的带，其中特定抗原与牙周炎患者的血清反应更频繁。监测抗体对特定抗原的反应可能对评估人类牙周病的进展具有重要意义。6)采用免疫化学方法从晚期破坏性牙周炎（ADP）患者血清中筛选抗原反应基因克隆，构建放线菌Y4 DNA噬菌体文库。该研究发现了一种独特的DNA序列，编码对ADP患者血清有强烈反应的a.a.y 4抗原。因此，作为一个DNA探针在牙周炎的诊断。少

Our analysis concerning the microbiological and immunological mechanisms of periodontal diseases yielded many fruitful results. These results are as follows.1) Serum IgG antibodies of severe periodontitis patients were measured to seven periodontopathic bacteria with enzyme-linked immunosorbent assay(ELISA).The findings demonstrated the pathological role of B. gingivalis and A. actino mycetemcomitans played in periodontal disease.2) The degree of definitive neutrophil chemotaxis in Down' s syndrome(DS) and its relationship to periodontal disease progression was confirmed by our studies.3) Platelet-activating factor(PAF), a biological active lipid was detected in inflamed human gingival tissue directly associated with the occurrence and persistence of periodontal inflammation.4) Synthesis competence of 15-hydroxy-eicosatetraenoic acid(15-HETE) by polymorphonuclear leukocytes(PMLs) of severe periodontitis patients was measured to observe 15-lipoxygenase activity. Juvenile and rapidly pro … More gressive periodontitis groups reported lower 15-HETE synthesis than the age-matched controls. The results are relevant to those communications concerning juvenile and rapidly progressive periodontitis with abnormal PMNL function.5) Sonicated whole cell extracts and outer membrane proteins from B.gingivalis W50, Veionella purvula ATCC 17745 and A. actinomycetemcomitans ATCC 9710, and sonicated whole cell extract from B. gingivalis 381 were analyzed by the immunoblot technique using sera of patients with diverse periodontal diseases and control subjects.Several major bands to which the specific antigens reacted more frequently with sera from periodontitis patients were recorded. Monitoring of antibody response to specific antigens may be significant to assess human periodontal disease progress.6) Clones of genes with antigen reactive from sera of advanced destructive periodontitis(ADP) patients to construct a phage library of A.actinomycetemcomitans Y4 DNA were screened by the immunochemical analysis. This investigation indicated a distinctive DNA sequence encoding an A.a. Y4 antigen strongly reactive to the sera of the ADP patients. Hence, serve as a DNA probe in diagnosis of periodontitis. Less