The immunological investigations concerning the pathological mechanisms of periodontal diseases

牙周病病理机制的免疫学研究

• 批准号: 63480420
• 负责人: ISHIKAWA Isao
• 金额: $ 4.42万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480420/
• 关键词: ELISA method; Serum IgG antibody; Leukocytes function; Platelet-activating-factor (PAF); 15-HETE; Immunoblotting; Gene cloning; DNA Probe; 重度歯周炎; 歯周病原性菌; 生体応答; 抗体価; 走化能; 殺菌能

Our analysis concerning the microbiological and immunological mechanisms of periodontal diseases yielded many fruitful results. These results are as follows.1) Serum IgG antibodies of severe periodontitis patients were measured to seven periodontopathic bacteria with enzyme-linked immunosorbent assay(ELISA).The findings demonstrated the pathological role of B. gingivalis and A. actino mycetemcomitans played in periodontal disease.2) The degree of definitive neutrophil chemotaxis in Down' s syndrome(DS) and its relationship to periodontal disease progression was confirmed by our studies.3) Platelet-activating factor(PAF), a biological active lipid was detected in inflamed human gingival tissue directly associated with the occurrence and persistence of periodontal inflammation.4) Synthesis competence of 15-hydroxy-eicosatetraenoic acid(15-HETE) by polymorphonuclear leukocytes(PMLs) of severe periodontitis patients was measured to observe 15-lipoxygenase activity. Juvenile and rapidly pro … More gressive periodontitis groups reported lower 15-HETE synthesis than the age-matched controls. The results are relevant to those communications concerning juvenile and rapidly progressive periodontitis with abnormal PMNL function.5) Sonicated whole cell extracts and outer membrane proteins from B.gingivalis W50, Veionella purvula ATCC 17745 and A. actinomycetemcomitans ATCC 9710, and sonicated whole cell extract from B. gingivalis 381 were analyzed by the immunoblot technique using sera of patients with diverse periodontal diseases and control subjects.Several major bands to which the specific antigens reacted more frequently with sera from periodontitis patients were recorded. Monitoring of antibody response to specific antigens may be significant to assess human periodontal disease progress.6) Clones of genes with antigen reactive from sera of advanced destructive periodontitis(ADP) patients to construct a phage library of A.actinomycetemcomitans Y4 DNA were screened by the immunochemical analysis. This investigation indicated a distinctive DNA sequence encoding an A.a. Y4 antigen strongly reactive to the sera of the ADP patients. Hence, serve as a DNA probe in diagnosis of periodontitis. Less

我们对牙周病的微生物学和免疫学机制的分析取得了许多丰硕的成果。结果如下：1)采用酶联免疫吸附试验(ELISA)测定了重度牙周炎患者血清中7种牙周病菌的IgG抗体，结果证实了牙龈芽孢杆菌和伴放线菌在牙周病中的病理作用。2)唐氏综合征(DS)和中性粒细胞的明确趋化程度。 我们的研究证实了其与牙周疾病进展的关系。3）在发炎的人类牙龈组织中检测到了血小板激活因子（PAF），这是一种生物活性脂质，与牙周炎症的发生和持续直接相关。4）多形核白细胞（PML）合成15-羟基二十碳四烯酸（15-HETE）的能力 测量了严重牙周炎患者的15-脂氧合酶活性。青少年和快速进展性牙周炎组报告的 15-HETE 合成低于年龄匹配的对照组。结果与有关 PMNL 功能异常的青少年和快速进展性牙周炎的交流相关。5) 使用免疫印迹技术，通过免疫印迹技术分析了来自 B.gingivalis W50、Veionella purvula ATCC 17745 和 A. actinomycetemcomitans ATCC 9710 的超声处理的全细胞提取物和外膜蛋白，以及来自 B. gingivalis 381 的超声处理的全细胞提取物。 患有不同牙周病的患者和对照受试者的血清。记录了牙周炎患者血清中特定抗原更频繁反应的几个主要条带。监测抗体对特定抗原的反应可能对评估人类牙周病的进展具有重要意义。6)通过免疫化学分析筛选晚期破坏性牙周炎(ADP)患者血清中与抗原反应的基因克隆，构建伴放线菌Y4 DNA噬菌体文库。这项研究表明了编码 A.a. 的独特 DNA 序列。 Y4抗原与ADP患者的血清有强烈反应。因此，可作为诊断牙周炎的DNA探针。较少的

项目成果

K.Noguchi, I.Morita, I.Ishikawa and S.Murota.: "Impaired polymorphonuclear leukocyte 15-lipoxygenase activity in juvenile and rapidly progressive periodontitis." Prostagrandins Leukotrienes and Fatty Acids. 33:137-141, 1988.

K.Noguchi、I.Morita、I.Ishikawa 和 S.Murota.：“青少年和快速进展性牙周炎中多形核白细胞 15-脂氧合酶活性受损。”

H.Watanabe, P.D.Marsh and L.Ivanyi.: "Detection of Immunodominant antigens of periodontopathic bacteria in human periodontal disease." Oral Microbiological Immunology. 4:159-164, 1989.

H.Watanabe、P.D.Marsh 和 L.Ivanyi.：“检测人类牙周病中牙周病细菌的免疫显性抗原。”

M.Umeda: "Improved detection of oral spirochetes with an anaerobic culture method" Oral Microbiology and Immunology. 5. (1990)

M.Umeda：“用厌氧培养方法改进口腔螺旋体的检测”口腔微生物学和免疫学。

荒川真一: "重度進行性歯周炎患者末梢血血清と反応するActinobacillus actinomycetemcomitans Y4株抗原遺伝子のクロ-ニング" 口腔病学会雑誌. 56. 494-512 (1989)

Shinichi Arakawa：“与严重进行性牙周炎患者的外周血清发生反应的放线放线杆菌Y4株抗原基因的克隆”口腔学会杂志56。494-512（1989）。

K.Noguchi, I.Morita and S.Murota.: "The detection of platelet-activating factor in inflamed human gingival tissue." Archives of Oral Biology. 34:37-41, 1989.

K.Noguchi、I.Morita 和 S.Murota.：“在发炎的人类牙龈组织中检测血小板激活因子。”