The immunological investigations concerning the pathological mechanisms of periodontal diseases

我们对牙周病的微生物学和免疫学机制的分析取得了许多丰硕的成果。1)用酶联免疫吸附试验检测了重度牙周炎患者血清中7种牙周病原菌的抗体。结果表明，牙龈假单胞菌和放线放线菌在牙周病中起到了病理作用。2)中性粒细胞趋化在唐氏S综合征(DS)中的程度及其与牙周疾病进展的关系。3)血小板激活因子(PAF)，4)检测重度牙周炎患者中性粒细胞(PML)合成15-羟基二十碳四烯酸(15-HETE)的能力，观察15-脂氧合酶活性。青少年和迅速支持…的人与年龄匹配的对照组相比，进展性牙周炎组的15-HETE合成较低。5)应用免疫印迹技术，对牙周炎患者血清和对照组血清进行了超声全细胞提取液和外膜蛋白的免疫印迹分析，记录了牙周炎患者血清中特异性抗原与牙周炎患者血清反应频率较高的几条主带。6)从晚期破坏性牙周炎(ADP)患者血清中筛选具有抗原反应的基因克隆，构建放线菌Y4DNA噬菌体文库。这项研究表明，一个编码A.A.的独特DNA序列。Y4抗原与ADP患者血清呈强阳性反应。因此，可作为牙周炎诊断的DNA探针。较少

Our analysis concerning the microbiological and immunological mechanisms of periodontal diseases yielded many fruitful results. These results are as follows.1) Serum IgG antibodies of severe periodontitis patients were measured to seven periodontopathic bacteria with enzyme-linked immunosorbent assay(ELISA).The findings demonstrated the pathological role of B. gingivalis and A. actino mycetemcomitans played in periodontal disease.2) The degree of definitive neutrophil chemotaxis in Down' s syndrome(DS) and its relationship to periodontal disease progression was confirmed by our studies.3) Platelet-activating factor(PAF), a biological active lipid was detected in inflamed human gingival tissue directly associated with the occurrence and persistence of periodontal inflammation.4) Synthesis competence of 15-hydroxy-eicosatetraenoic acid(15-HETE) by polymorphonuclear leukocytes(PMLs) of severe periodontitis patients was measured to observe 15-lipoxygenase activity. Juvenile and rapidly pro … More gressive periodontitis groups reported lower 15-HETE synthesis than the age-matched controls. The results are relevant to those communications concerning juvenile and rapidly progressive periodontitis with abnormal PMNL function.5) Sonicated whole cell extracts and outer membrane proteins from B.gingivalis W50, Veionella purvula ATCC 17745 and A. actinomycetemcomitans ATCC 9710, and sonicated whole cell extract from B. gingivalis 381 were analyzed by the immunoblot technique using sera of patients with diverse periodontal diseases and control subjects.Several major bands to which the specific antigens reacted more frequently with sera from periodontitis patients were recorded. Monitoring of antibody response to specific antigens may be significant to assess human periodontal disease progress.6) Clones of genes with antigen reactive from sera of advanced destructive periodontitis(ADP) patients to construct a phage library of A.actinomycetemcomitans Y4 DNA were screened by the immunochemical analysis. This investigation indicated a distinctive DNA sequence encoding an A.a. Y4 antigen strongly reactive to the sera of the ADP patients. Hence, serve as a DNA probe in diagnosis of periodontitis. Less