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Studies on immunological diagnosis of periodontal diseases

牙周病的免疫学诊断研究

基本信息

批准号：
02454433
负责人：
ISHIKAWA Isao
金额：
$ 4.35万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The relationship between the periodontopathic bacteria and host resistance in periodontal diseases has been investigated microbiologically, immunologically and genetically. The serum IgG antibody titers were measured by using an enzyme-linked immunosorbent assay(ELISA) to investigate the diagnostic value in order to find main periodontopathic bacteria, and to evaluate the clinical prognosis. A new method of ELISA measurement was developed to improve the efficiency. Using this new method, the serum IgG antibody titers to Porphyromonas gingivalis (P.g.), Prevotella intermedia (P.i.), Actinobacillus actinomycetemcomitans (A.a.) and Fusobacterium nucleatum were evaluated. The results indicated that this improved method showed 6 times more efficient in managing subjects as compared to the former one and might be effective for screening of serum with high antibody titers. The IgG antibody titers against P.g. and P.i. decreased significantly after periodontal treatment. There is a significant … More correlation between the reduction of antibody activity to P.g. and the frequency of periodontal surgeries or the duration of treatment. The serum antibody titers to P.i. was detected to reduce significantly in relation to the number of lost teeth. These results suggest that the reduction of the serum antibody titers to P.g. and P.i. can be used as parameters to show effectiveness of periodontal treatment. We also analyzed the relation between the antibody titers and clinical parameters about 60 patients with periodontal disease measured for two years from 1988 to 1989. As results, the serum antibody activity to P.g. was significantly correlated with the probing depth and the rate of alveolar bone resorption. The antibody titers to A.a. was also correlated with the rate of alveolar bone resorption. These findings suggest that P.g. and A.a. are key bacteria to be responsible for the development of periodontitis. Futhermore, non-radioactive DNA probes for periodontopathic pathogens, such as P.g. or P.i., were developed. These probes showed sufficient sensitivity and specificity for clinical trials. By using the DNA probe, P.g. was detected from all patients examined and the frequency of P.g. detection increased significantly in the sites with deep probing depth or bleeding on probing. These results indicate that P.g. may play an important role in adult periodontitis. On the other hand, using RFLPs analysis we examined P.g. strains on their fimbrillin gene locus. The results indicate that genetic heterogeneity seems to be existing within the fimbrillin gene locus. This may also reflect the difference of virulence within P.g. species reported before. Less

牙周病中牙周病细菌与宿主抵抗力之间的关系已从微生物学、免疫学和遗传学方面进行了研究。采用酶联免疫吸附试验（ELISA）测定血清IgG抗体滴度，探讨其诊断价值，以寻找主要牙周病菌，评估临床预后。开发了一种新的 ELISA 测量方法以提高效率。使用这种新方法，评估了针对牙龈卟啉单胞菌 (P.g.)、中间普雷沃氏菌 (P.i.)、伴放线放线杆菌 (A.a.) 和具核梭杆菌的血清 IgG 抗体滴度。结果表明，与前一种方法相比，这种改进的方法在管理受试者方面的效率提高了 6 倍，并且可能有效地筛选高抗体滴度的血清。针对 P.g. 的 IgG 抗体效价和 P.i。牙周治疗后明显下降。 P.g. 抗体活性降低之间存在显着相关性。以及牙周手术的频率或治疗的持续时间。 P.i 血清抗体滴度据检测，牙齿脱落的数量显着减少。这些结果表明 P.g. 血清抗体滴度的降低。和 P.i。可以作为显示牙周治疗效果的参数。我们还分析了1988年至1989年两年间测定的60名牙周病患者的抗体滴度与临床参数之间的关系。与探诊深度和牙槽骨吸收率显着相关。 A.a 的抗体滴度也与牙槽骨吸收率相关。这些发现表明 P.g.和 A.a.是导致牙周炎发生的关键细菌。此外，针对牙周病病原体的非放射性 DNA 探针，例如 P.g.或 P.i.，被开发出来。这些探针对临床试验表现出足够的敏感性和特异性。通过使用 DNA 探针，P.g.从所有接受检查的患者中检测到 P.g. 的频率。在探诊深度较深或探诊出血的部位，检出率显着增加。这些结果表明 P.g.可能在成人牙周炎中发挥重要作用。另一方面，使用 RFLP 分析，我们检查了 P.g.菌株的 fimbrillin 基因位点。结果表明，fimbrillin 基因座内似乎存在遗传异质性。这也可能反映了P.g.内毒力的差异。之前报道过的物种。较少的