Studies on immunological diagnosis of periodontal diseases

从微生物学、免疫学和遗传学的角度研究了牙周病中牙周病原菌与宿主耐药性的关系。采用酶联免疫吸附试验(EL ISA)检测血清免疫球蛋白抗体效价，探讨其对牙周病的诊断价值，以寻找牙周主要致病菌，并对临床预后进行评估。为提高检测效率，建立了一种新的酶联免疫吸附测定方法。用这种新方法，血清中抗牙龈卟啉单胞菌(P.G.)、中间普里沃特氏菌(P.I.)、伴生放线杆菌(A.A.)的抗体效价均较高。和核梭杆菌。结果表明，改进后的方法对受试者的管理效率是原方法的6倍，可用于高滴度血清的筛选。抗P.G.的抗体效价较高。私家侦探。牙周治疗后较治疗前明显下降。有一个重要的…与P.G.抗体活性降低之间的相关性更强。以及牙周手术的频率或治疗时间。血清中抗P.I.抗体效价较高。被检测到与脱牙数量相关的显著减少。这些结果表明，Pg血清抗体效价的降低。私家侦探。可作为反映牙周治疗效果的参数。并对1988~1989年两年间检测的60例牙周病患者的抗体滴度与临床参数的关系进行了分析。结果表明，血清中抗P.G.与牙槽骨密度、牙槽骨吸收率呈显著正相关。抗体效价为A.A。也与牙槽骨吸收率相关。这些发现表明，P.G.和戒酒协会。是导致牙周炎的关键细菌。此外，用于牙周病病原体的非放射性DNA探针，如P.G.或者私家侦探，都是被开发出来的。这些探针在临床试验中表现出足够的敏感性和特异性。通过使用DNA探针P.G.从所有被检查的患者中检测到Pg。探诊深度较深或探诊时出血的部位，探查阳性率明显增加。这些结果表明，P.G.可能在成人牙周炎中起重要作用。另一方面，利用RFLP分析，我们检验了P.G.他们的fimbrins基因座上的菌株。结果表明，fimbrins基因座内似乎存在遗传异质性。这也可能反映了P.G.内部毒力的差异。以前报道过的物种。较少

The relationship between the periodontopathic bacteria and host resistance in periodontal diseases has been investigated microbiologically, immunologically and genetically. The serum IgG antibody titers were measured by using an enzyme-linked immunosorbent assay(ELISA) to investigate the diagnostic value in order to find main periodontopathic bacteria, and to evaluate the clinical prognosis. A new method of ELISA measurement was developed to improve the efficiency. Using this new method, the serum IgG antibody titers to Porphyromonas gingivalis (P.g.), Prevotella intermedia (P.i.), Actinobacillus actinomycetemcomitans (A.a.) and Fusobacterium nucleatum were evaluated. The results indicated that this improved method showed 6 times more efficient in managing subjects as compared to the former one and might be effective for screening of serum with high antibody titers. The IgG antibody titers against P.g. and P.i. decreased significantly after periodontal treatment. There is a significant … More correlation between the reduction of antibody activity to P.g. and the frequency of periodontal surgeries or the duration of treatment. The serum antibody titers to P.i. was detected to reduce significantly in relation to the number of lost teeth. These results suggest that the reduction of the serum antibody titers to P.g. and P.i. can be used as parameters to show effectiveness of periodontal treatment. We also analyzed the relation between the antibody titers and clinical parameters about 60 patients with periodontal disease measured for two years from 1988 to 1989. As results, the serum antibody activity to P.g. was significantly correlated with the probing depth and the rate of alveolar bone resorption. The antibody titers to A.a. was also correlated with the rate of alveolar bone resorption. These findings suggest that P.g. and A.a. are key bacteria to be responsible for the development of periodontitis. Futhermore, non-radioactive DNA probes for periodontopathic pathogens, such as P.g. or P.i., were developed. These probes showed sufficient sensitivity and specificity for clinical trials. By using the DNA probe, P.g. was detected from all patients examined and the frequency of P.g. detection increased significantly in the sites with deep probing depth or bleeding on probing. These results indicate that P.g. may play an important role in adult periodontitis. On the other hand, using RFLPs analysis we examined P.g. strains on their fimbrillin gene locus. The results indicate that genetic heterogeneity seems to be existing within the fimbrillin gene locus. This may also reflect the difference of virulence within P.g. species reported before. Less