Immunological and molecular biological study of cytokine-network in periodontitis

为了阐明细胞因子在牙周炎中的细胞因子网络，我们研究了细胞因子在牙周病中的局部和系统作用。辅助T细胞调节免疫反应，分为1型辅助T细胞(Th1)和2型辅助T细胞(Th2)。Th1增强细胞免疫应答，Th2帮助体液免疫应答。这种差异是由细胞因子的产生决定的，例如Th1产生干扰素-γ和IL-2，Th2产生IL-4、IL-5、IL-6和IL-10。我们今年的研究目的是探讨牙周病细菌的免疫反应是否存在个体差异，以及个体差异如何影响牙周炎的临床状态。干扰素-γ的产生因个体而异。干扰素-γ的产生依赖于单核细胞产生IL-12的能力，由于干扰素-γ的高、低分泌水平对牙周炎的严重程度没有差别，因此我们不能根据Th1或Th2的极化来确定牙周炎的易感性，尽管对牙周病细菌的免疫反应因人而异。

We have examined local and systematic function of cytokines against periodontopathic bacteria to clarify cytokine-network in periodontitis.Helper T cells, which regulate immune responses, have been divided into Type1 helper T cell(Th1)and Type2 helper T cell(Th2). Th1 augments cell-mediated immune responses, and Th2 helps humoral immune responses. This difference is determined by cytokine production, for example, Th1 produces IFN-γ and IL-2, Th2 produces IL-4, IL-5, IL-6 and IL-10. The aim of our study in this year was to examine whether individual diversities exist on not in immune responses against periodontopathic bacteria, and how individual diversities affect the clinical status in periodontitis.Periheral blood T cells produce IFN-γ by stimulation with periodontopathic bacteria. IFN-γ production varied among individuals. The production of IFN-γ was dependent on the ability of monocytes to produce IL-12.As the severity of periodontitis was not different between IFN-γ high and low producer, we could not determine the susceptibility of periodontitis based on the polarization of Th1 or Th2, although immune response against periodontopathic bacteria varied among individuals.