Establishment of periodontal regenerative therapy using BMPs

采用包括双相磷酸钙（biphasic calcium phosphate， BCP）在内的三种不同载体将RhBMP-2植入大鼠皮下组织，对异位骨形成进行组织学评价。BCP-BMP组4个标本均能形成骨样组织，其中2个标本能形成具有片状结构的新骨。对照组未见骨样组织形成。采用RhBMP-2/PLGA明胶海绵修复大鼠下颌骨骨缺损。4周后处死，组织学观察骨形成情况。在8个实验样品中，有4个样品的缺损被新形成的骨完全填充，而对照组的新骨形成非常有限。与对照部位相比，rhbmp -2植入部位的新骨形成量显著增加。6只beagle犬双侧下第2、第3、第4前磨牙形成贯通性分叉缺损。RhBMP-2/PGS在实验部位植入较多，而PGS单独植入对照部位。10周时进行组织学评价。实验部位的分叉缺陷大部分被新形成的骨完全填充。与对照组相比，实验组的新骨和结缔组织附着量明显增加。两组间新牙骨质形成量无显著差异。在实验组和对照组中，大量新骨形成的部位均表现为强直。在6只恒河猴的双侧下第一、第二磨牙处制造贯通性功能缺损，并于12周时采用与比格犬实验相同的设计进行组织学评价。实验组的新骨形成量明显更大。实验部位的12个缺损中有2个完全被新骨填充，对照部位的12个缺损中有1个完全被新骨填充。未见强直。提示rhBMP-2具有异位骨形成的作用，促进牙周组织再生。少

RhBMP-2 was implanted to subcutaneous tissue of rats with three different types of carriers, including biphasic calcium phosphate (BCP), to evaluate ectopic bone formation histologically. All four samples of BCP-BMP group demonstrated bone-like tissue formation, and two of four demonstrated new bone formation with lamellae-like structure. No bone-like tissue formation was found in control groups.RhBMP-2/PLGA gelatin sponge was implanted in bone defects in rat mandible. The animals were sacrificed at four weeks and the bone formation was evaluated histologically. The defects were completely filled with newly formed bone in four samples out of eight experimental ones, while new bone formation was very limited in the control group. The amount of the new bone formation was significantly larger in the rhBMP-2-implanted sites as compared to the control sites.Through-and-through furcation defects were created at bilateral lower 2nd, 3rd, and 4th premolars in six beagle dogs. RhBMP-2/PGS was i … More mplanted in the experimental sites, whereas PGS alone was implanted in control sites. Histological evaluation was performed at ten weeks. Most of the furcation defects in the experimental sites were completely filled with newly formed bone. Significantly larger amounts of new bone and connective tissue attachment were shown in the experimental group as compared to the control group. No significant difference could be found in the amount of new cementum formation between the groups. Sites with large amount of new bone formation demonstrated ankylosis in both the experimental and the control groups.Through-and-through furcation defects were made at bilateral lower 1st and 2nd molars of six rhesus monkeys, and histological evaluation was performed at 12 weeks with the same design as the experiment using beagle dogs. Amounts of new bonformation were significantly larger in the experimental group. Two out of twelve defects in the experimental sites were completely filled with new bone, and one of twelve in control sites was. No ankylosis was observed.These results indicate that rhBMP-2 has an effect of ectopic bone formation, and promote periodontal tissue regeneration. Less