Freeze-preservation of useful genetic resources of horticultural crops

1.冻结-保存可再生百合泡>条件描述以下是合适的：组织片的大小，约2毫米; DMSO的集中和上传时间，16%和30分钟;预冻结，冷却到-30 ° c在1.0-2.0 ° c/min。在2周之前冻结冷却处理在0 ° c对液体氮素中的生存有效。这证实了这些条件在液体硝酸盐和6个月的维护中能够达到80%的存活率。冻结-保存pear shoot tips：“Flemish Beauty”，收集在December or February，survive after being freezed in liquid nitrogen， while of those collected on March 20 are killed.3。Freeze-preservation of pear shoot tips： With shoot tips of“delawere”、“Buffalo”和“Campbell Early”， collected on November 12， survival rates in freezing are 100% at-20 c， and shown that freezing resistance of materials collected on November 12高于September 12。关于De的射击技巧 ... More 在液体硝酸盐中冻结的情况下，有很高的存活率（100%）。冻结苹果射击技巧：自然射击技巧在-196 ° C冻结的生存率如下：在“夏季”射击技巧收集了3天不同的时间，70%在4月8日，50%在4月18日，25%在4月28日;在“斯巴达”中，4月8日上涨90%，4月18日上涨50%，4月28日上涨25%，自然射击技巧的生存率（来自孤儿森林的精选收藏于3月19日，储存于0 ° C;冻结于液体硝酸盐）如下：从3月29日到5月27日，70%与“Summerland”，100%与“Spartan”，90%与“Starking Delicious”。Kuro-mi-no-uguisu-kagura射击技巧的体外培养（Lonicera caerulea L.）var. Emphyllocalyx NAKAI）作为冷冻保存的必要技术： BA、2 ip和GA添加到中间，增加了叶子的数量和长度。BA比2 ip提供更多的细胞素，因为它在钙状形成中的轻微增强。调查员打算进一步开发这一研究。Less（低）

1. Freeze-preservation of apical meristem of edible lily bulblest> Conditions described below are suitable : size of a tissue piece,about 2mm; concentration and uptake time of DMSO, 16% and 30min; prefreezing,cooling down to -30゜c at 1.0-2.0゜c/min. Before-freezing cooling treatment at 0゜c for 2 weeks is effective for survival in liquid nitrogen. It is confirmed that these conditions enable survival of 80% in liquid nitrogen and 6-month maintenance of it.2. Freeze-preservation of pear shoot tips: Almost all shoot tips of "Flemish Beauty", collected in December or February,survive after being freezed in liquid nitrogen, while all of those collected on March 20 are killed.3. Freeze-preservation of pear shoot tips: With shoot tips of "delawere","Buffalo" and "Campbell Early", collected on November 12, survival rates in freezing are 100% at -20゜c and 70% at -30- -40゜c, and shown that freezing resistance of materials collected on November 12 is higher than that in September. Shoot tips on De … More cember 13 have high survival rate (100%) in case of being freezed in liquid nitrogen.4. Freeze-preservation of apple shoot tips: Survival rates of natural shoot tips freezed at -196゜c are as follows: in "Summerland" shoot tips collected on 3 different days, 70% on April 8, 50% on April 18, 25% on April 28; in"Spartan", 90% on April 8, 50% on April 18, 25% on April 28,Survival rates of natural shoot tips(excised from the dormant woods which are collected on March 19 and stored at 0゜c; freezed in liquid nitrogen) are as follows: from March 29 through May 27, above 70% with "Summerland", 100% with"Spartan", 90% with"Starking Delicious".5. In-vitro culture of shoot tips of Kuro-mi-no-uguisu-kagura(Lonicera caerulea L. var. emphyllocalyx NAKAI) as a necessary technique for rreeze-preservation: BA, 2ip and GA added to medium, enhance the number and length of leaves. BA is more available cytokinin than 2ip, because of little enhancement in callus formation. The investigator intend to develop this research further. Less