Freeze-preservation of useful genetic resources of horticultural crops

园艺作物有用遗传资源的冷冻保存

• 批准号: 61480039
• 负责人: HARADA Takashi
• 金额: $ 3.39万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480039/
• 关键词: Genetic resources; Freeze-preservation; Edible lily; Pear; Grape; Apple; クロミノウグイスカグラ; ラズベリー; ニンニク; ジャガイモ

1. Freeze-preservation of apical meristem of edible lily bulblest> Conditions described below are suitable : size of a tissue piece,about 2mm; concentration and uptake time of DMSO, 16% and 30min; prefreezing,cooling down to -30゜c at 1.0-2.0゜c/min. Before-freezing cooling treatment at 0゜c for 2 weeks is effective for survival in liquid nitrogen. It is confirmed that these conditions enable survival of 80% in liquid nitrogen and 6-month maintenance of it.2. Freeze-preservation of pear shoot tips: Almost all shoot tips of "Flemish Beauty", collected in December or February,survive after being freezed in liquid nitrogen, while all of those collected on March 20 are killed.3. Freeze-preservation of pear shoot tips: With shoot tips of "delawere","Buffalo" and "Campbell Early", collected on November 12, survival rates in freezing are 100% at -20゜c and 70% at -30- -40゜c, and shown that freezing resistance of materials collected on November 12 is higher than that in September. Shoot tips on De … More cember 13 have high survival rate (100%) in case of being freezed in liquid nitrogen.4. Freeze-preservation of apple shoot tips: Survival rates of natural shoot tips freezed at -196゜c are as follows: in "Summerland" shoot tips collected on 3 different days, 70% on April 8, 50% on April 18, 25% on April 28; in"Spartan", 90% on April 8, 50% on April 18, 25% on April 28,Survival rates of natural shoot tips(excised from the dormant woods which are collected on March 19 and stored at 0゜c; freezed in liquid nitrogen) are as follows: from March 29 through May 27, above 70% with "Summerland", 100% with"Spartan", 90% with"Starking Delicious".5. In-vitro culture of shoot tips of Kuro-mi-no-uguisu-kagura(Lonicera caerulea L. var. emphyllocalyx NAKAI) as a necessary technique for rreeze-preservation: BA, 2ip and GA added to medium, enhance the number and length of leaves. BA is more available cytokinin than 2ip, because of little enhancement in callus formation. The investigator intend to develop this research further. Less

1.百合鳞茎顶端分生组织的冷冻保存适宜的条件为：组织块大小2 mm左右，DMSO浓度16%，吸收时间30 min，预冻，以1.0-2.0 ℃/min的速度降温至-30 ℃，冷冻前0 ℃低温处理2周，液氮保存效果较好。经证实，这些条件使液氮中80%的存活率能够维持6个月。2.梨茎尖的冷冻保存：在12月或2月采集的“佛兰芒美人”的茎尖，几乎所有的茎尖在液氮中冷冻后都能存活，而在3月20日采集的所有茎尖都被杀死。梨茎尖的冷冻保存：11月12日采的“delaware”、“布法罗”和“坎贝尔Early”的茎尖，在-20 ℃下的冷冻保存率为100%，在-30 ~-40 ℃下的冷冻保存率为70%，表明11月12日采的材料的抗冻性高于9月采的材料。拍摄技巧上德 ...更多信息 在液氮中冻存的情况下，cn 13的存活率很高（100%）.苹果茎尖的冷冻保存：自然茎尖在-196 ℃下冷冻保存的成活率如下：“夏地”3天采集的茎尖，4月8日为70%，4月18日为50%，4月28日为25%;“斯巴达”4月8日成活率90%，4月18日成活率50%，4月28日成活率25%，（从休眠的木材中切除，其在3月19日采集并储存在0 ℃;在液氮中冷冻）如下：从3月29日到5月27日，70%以上的人选择了“夏日乐园”，100%的人选择了“斯巴达”，90%的人选择了“美味Starking”。以紫茎泽兰茎尖为外植体，进行离体培养。变种在培养基中添加BA、2 ip和GA，可增加叶片的数量和长度。BA对愈伤组织形成的促进作用不大，是比2 ip更有效的细胞分裂素。研究者打算进一步开展这项研究。少

项目成果

鈴木卓: 北海道大学農学部邦文紀要. 15. 118-123 (1987)

铃木隆：日本文学通报，北海道大学农学部，15. 118-123 (1987)。

Takashi,SUZUKI; Takashi,HARADA; Toshiro,YAKUWA: "Studies on freeze-preservation of fruit tree germplasm 1. Survival of pear leaf bud apices frozen in liquidN" Memoirs of the faculty of agriculture, Hokkaido University. 15. 118-123 (1987)

铃木隆；