权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Comparative study on the tumorigenicity of gallium arsenide,gallium phosphide and gallium oxide in hamsters following intratracheal instillation or in mice following intraperitoneal and subcutaneous injection.

砷化镓、磷化镓、氧化镓对仓鼠气管内滴注及小鼠腹腔及皮下注射致瘤性的比较研究

基本信息

批准号：
61480165
负责人：
HISANAGA Akira
金额：
$ 4.54万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

Tumorigenicity of gallium arsenide (GaAs) and gallium phosphide (GaP) was studied in male Syrian golden hamsters or male ICR mice.In GaAs imtratracheal instillation study, GaAs was instilled into the lungs of male Syrian golden hamsters by intratracheal instillations once a week for 15 week. The total dosage were 3.75 mg Ss. Although GaAs had no apparant tumorigenicity, GaAs particulates were likely to produce relatively severe damage and the survival of the animals was shortened significantly compared with the control group.In GaAs, GaP and Ga_2O_3 subcutaneous or intraperitineal injection study, male ICR mice received once by a subcutaneous or intraperitoneal injection of each compound. the total doses given were 48 mg Ga/kg or 480 mg Ga/kg of each drug suspended in 0.2 ml of olive oil. No tumors were observed in the subcutis of the back injected, and there was no significant difference concerning the survival periods of each group compared with the control group following the subcut … More aneous injection. The total tumor incidence ub the Ga_2O_3 (480 mg Ga/kg) group of intraperitoneal injection was significantly different compared with the control group. In the GaAs (480 mg Ga/kg) and Ga_2O_3 (48 mg Ga/kg) group of intraperitoneal injection, the survival days of the mice were shortaned significantly compared with the control group. Trom this study, although it was not claear whether this increased production of total tumor in the Ga_2O_3 (480 mg Ga/kg) group was due to tumorigenic action of Ga_2O_3 itself or not, it appears that GaAs and Ga_2O_3 revealed potential systemic toxicity in mice following the intraperitoneal injection.In the morphological study, we investigated the light and electron microscopical changes of the lung in the hamsters instilled indium arsenide (InAs) or indium phosphide (InP) intratracheally. In the light microscopical findings, the prominent change observed in the lungs was marked proliferation of alveolar epitheliums on the alveolar wall and respiratory bronchiolar epitheliums, which extended towards alveolar ducts and neighboring alveoli. In the scanning electron microscopic findings, the main change observed in the alveolar region was the deposition of InAs or InP particulates, the accumulation of macrophages in the alveoli and the thickening the alveolar walls, which lesions were more frequently seen in the alveolar region than in the bronchiolar region. Less

在雄性叙利亚金仓鼠或雄性ICR小鼠中研究了砷化镓（GaAs）和磷化镓（GaP）的致瘤性。在GaAs气管内滴注研究中，每周一次将GaAs通过气管内滴注的方式注入雄性叙利亚金仓鼠的肺部，持续15周。总剂量为3.75 mg Ss。虽然GaAs没有明显的致瘤性，但GaAs颗粒很可能产生相对严重的损伤，与对照组相比，动物的存活时间明显缩短。在GaAs、GaP和Ga_2O_3皮下或腹腔注射研究中，雄性ICR小鼠分别皮下或腹腔注射各化合物一次。每种药物的总剂量为 48 mg Ga/kg 或 480 mg Ga/kg，悬浮在 0.2 ml 橄榄油中。注射后背部皮下组织未见肿瘤，各组经皮下注射后的生存期与对照组相比无显着性差异。 Ga_2O_3(480 mg Ga/kg)腹腔注射组的肿瘤总发生率与对照组相比有显着性差异。腹腔注射GaAs(480 mg Ga/kg)和Ga_2O_3(48 mg Ga/kg)组小鼠的存活天数较对照组显着缩短。从这项研究来看，虽然尚不清楚Ga_2O_3（480 mg Ga/kg）组中总肿瘤产量的增加是否是由于Ga_2O_3本身的致瘤作用所致，但似乎GaAs和Ga_2O_3在腹腔注射后显示出小鼠潜在的全身毒性。在形态学研究中，我们研究了小鼠肺部的光和电子显微镜变化。仓鼠气管内注入砷化铟（InAs）或磷化铟（InP）。在光镜下观察到，肺部的显着变化是肺泡壁上的肺泡上皮和呼吸性细支气管上皮明显增殖，并向肺泡管和邻近的肺泡延伸。扫描电镜结果显示，肺泡区的主要变化是InAs或InP颗粒的沉积、巨噬细胞在肺泡内的积累以及肺泡壁的增厚，这些病变在肺泡区比在细支气管区更常见。较少的