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Study of the responsibility on the developing vitreous and retina.

研究玻璃体和视网膜发育的责任。

基本信息

批准号：
61480369
负责人：
UEMURA Yasuo
金额：
$ 4.29万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

1. The eyes of rat, mouse and rabbit were quite different from human eyes by comparative anatomic analysis of ciliary body, peripheral retina, and the base of vitreous. The eyes of dog and cat were similar to human eyes. Although the monkey's eye resembles human eye most, the development of retinal vessels are mature at birth, suggesting that this is not an appropriate animal model.2. Ciliary epithelium of the human fetus eye was partially stained by S100 protein antiserum, but not stained by GFAP suggesting that the ciliary epithelium have no glial cell origin.3. Oxygen induced retinopathy of dog were most produced when 90-100% oxygen were provided. The proli- ferative retinal vessels on the optic disc healed without formation of retrolental fibroplasia or retinal folds.4. It became possible to culture the human ciliary epithelium separating pigment and non-pigment cells. In optimal culuture condition of nonpigmentary epithelium, the fetus bovine serum was important as cell growth factor as well as glucose and amino acids.5. A sedimentation line was formed between vitreous soluble protein and the bovine antivitreous serum after absorption of bovine serum ingredients. This protein was estimated to be 20,000 daltons.6. So called tertial vitreous began to form from the ciliary side at 9 weeks gestation period. After 12 weeks, Hyaluronic acid is produced for the vitreous formation by muller cells in retina.7. Hyalocyte like cells were observed in the human vitreous since the early period of development. These cells were found not only in front of the lens but also around the optic vessels.8. Lumen formation of proliferative cells was observed at the initial cell culture of endothelium of human umblical vessels.9. It was found that the application of sodium glutamic acid produced a microphthalmos with a variety of ocular abnormalities.

1. 通过对大鼠、小鼠和家兔眼睛的睫状体、周围视网膜和玻璃体基部的比较解剖分析，发现大鼠、小鼠和家兔的眼睛与人眼有很大的不同。狗和猫的眼睛与人的眼睛相似。虽然猴子的眼睛与人眼最相似，但视网膜血管在出生时发育成熟，这表明这不是一种合适的动物模型。2 . S100蛋白抗血清对人胎眼睫状上皮进行了部分染色，但GFAP未对其进行染色，提示睫状上皮无胶质细胞来源。氧致狗视网膜病变以90 ~ 100%供氧时发生最多。视盘上增生的视网膜血管愈合，未形成视网膜后纤维增生或视网膜褶皱。培养人纤毛上皮细胞分离色素细胞和非色素细胞成为可能。在非色素上皮的最佳培养条件下，胎牛血清作为细胞生长因子、葡萄糖和氨基酸具有重要的作用。牛血清成分吸收后，玻璃体可溶性蛋白与牛抗玻璃体血清之间形成沉淀线。这种蛋白质估计有20,000道尔顿。所谓的黄体玻璃体在妊娠9周时从纤毛侧开始形成。12周后，视网膜muller细胞产生玻璃体形成所需的透明质酸。从发育早期开始，在人玻璃体中就观察到类似透明细胞的细胞。这些细胞不仅存在于晶状体前，也存在于视血管周围。在人脐血管内皮细胞初始培养过程中，观察到增生细胞形成管腔。结果发现，应用谷氨酸钠可引起小眼，并伴有多种眼部异常。