Intracellular hyperthermia by magnetic submicron particles

我们报道了利用亚微米激发粒子进行感应加热的“主动热疗”。研制了一套500 KHZ的感应热疗系统，用于检测各种亚微米粒子的特性。在500 KHZ下，Fe_3O_4、Fe Fe Fe Metal、SUS 430 LAN、3C和右旋糖酐磁铁矿（Fe 207 mg/ml，右旋糖酐84 mg/ml）表现出良好的励磁特性，Fe_2O_3和Mn-Zn铁氧体在较低的励磁温度下表现出良好的励磁特性。500 KHZ频率的右旋糖酐磁铁矿（Dextran Magnetite）作为一种新的激发粒子，经腹腔注射，通过肿瘤细胞的吞噬作用，定位于AH 60 C腹水细胞的胞间。这些亚微米粒子在感应加热系统的磁场中被激发，与体外相同。在体内实验中，3C和右旋糖酐磁铁矿的作用效果最好。肿瘤细胞对粒子的选择性摄取是肿瘤细胞选择性热疗的可行途径。这种生物物理方法代表了有效治疗热疗的新技术，导致选择性破坏癌细胞，对周围正常细胞和组织的副作用很小。

We have reported "Active Hyperthermia" by means of inductive baeting using submicron excting perticles. An inductive hyperthermia system at 500KHZ to examine the characteristics of various submicron particles was prepared. At 500KHZ, Fe_3O_4, Fe Iron Metal, SUS 430LAN, 3C and Dextran Magnetite (Fe 207mg and Dextran 84mg/ml) showed good exciting characteristics, while Fe_2O_3 and Mn-Zn ferrite remainde at low exciting temparature. Dextran Magnetite, one of the new exciting particle, at a frequency of 500KHZ were very promising.The injected submicron particles intrapentonally, located interacellularlly in ascitic cells of AH60C by tumor cell phagocytosis. These submicron particles in tumor cells at the field of inductive heating system were excited as same as in vitro. In the experiment of in vivo using 3C and Dextran magnetite, comparing with other groups, were most effective. It is suggested that the selective uptake of the particles by tumor cells is practicable for selective hyperthermia of cancer cells. This biophysical approach represents new thechnology for effective treatment of hyperthermia, resulting in the selective destruction of cancer cells with little side effects on the sorrounding normal cells and tissue.