Intracellular hyperthermia by magnetic submicron particles

我们报道了利用亚微米激发粒子进行感应加热的“主动热疗”。制备了一种500KHZ感应热热系统，用于检测各种亚微米颗粒的特性。在500KHZ时，Fe_3O_4、Fe Iron Metal、SUS 430LAN、3C和Dextran磁铁矿（Fe 207mg和Dextran 84mg/ml）表现出良好的激发特性，而Fe_2O_3和Mn-Zn铁氧体在较低的激发温度下保持不变。右旋糖酐磁铁矿是一种新的激发粒子，在500KHZ的频率上很有前景。注射的亚微米颗粒经肿瘤细胞吞噬作用位于AH60C腹水细胞胞间。这些亚微米颗粒在感应加热系统场下的肿瘤细胞中受到与体外相同的激发。在体内实验中使用3C和右旋糖酐磁铁矿，与其他组比较，效果最好。结果表明，肿瘤细胞对该颗粒的选择性摄取可用于肿瘤细胞的选择性热疗。这种生物物理方法代表了有效治疗热疗的新技术，可以选择性地破坏癌细胞，对周围的正常细胞和组织几乎没有副作用。

We have reported "Active Hyperthermia" by means of inductive baeting using submicron excting perticles. An inductive hyperthermia system at 500KHZ to examine the characteristics of various submicron particles was prepared. At 500KHZ, Fe_3O_4, Fe Iron Metal, SUS 430LAN, 3C and Dextran Magnetite (Fe 207mg and Dextran 84mg/ml) showed good exciting characteristics, while Fe_2O_3 and Mn-Zn ferrite remainde at low exciting temparature. Dextran Magnetite, one of the new exciting particle, at a frequency of 500KHZ were very promising.The injected submicron particles intrapentonally, located interacellularlly in ascitic cells of AH60C by tumor cell phagocytosis. These submicron particles in tumor cells at the field of inductive heating system were excited as same as in vitro. In the experiment of in vivo using 3C and Dextran magnetite, comparing with other groups, were most effective. It is suggested that the selective uptake of the particles by tumor cells is practicable for selective hyperthermia of cancer cells. This biophysical approach represents new thechnology for effective treatment of hyperthermia, resulting in the selective destruction of cancer cells with little side effects on the sorrounding normal cells and tissue.