Analysis of anticarcinogenesis on apple pectin-mechanisum of inhibitory on free radical and DNA damage

苹果果胶抗癌作用分析——抑制自由基和DNA损伤的机制

• 批准号: 12671148
• 负责人: TAZAWA Kenji
• 金额: $ 2.11万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671148/
• 关键词: apple pectin; dietary fiber; free radical; hydroxyradical; small molecular oligosaccaride; ONOO^-; ROO・; DNA damage; マイクロチャンネルアレイ; 血液流動性; 暗視野顕微鏡; 赤血球連鎖; 活性酸素; 大腸がん予防; ペクチン; ヒドロキシラジカル; 大腸がん発生; アップルファイバー; 大腸癌予防; 大腸癌発生; スーパーオキサイドアニオンラジカル

By dividing the oligosaccharide of apple pectin derivation by mean degree of polymerization in 4 groups from 2000 ear, the effect for the prevention of the oxidative damage for DNA was examined. It was clarified that the scavenging for free radical was high method in each fractionation of the low-moleculer region using the ESR. The merchandizing of the apple pectin of this low-moleculer region was tried, and 22.5% inclusion low-moleculer oligosaccharide of D1 and D2 was developed. The high-functional apple pectin of low-moleculer oligosaccharide was taken to the human, and it was clarified that the blood flow velocity decreased how the hemodynamics is improved, using MC-FAOn scavenging activity for ONOO^-, it was proven that POS-S component was 2.4 (titer) and POS-H of heated POS-S was 80.8, when it was examined using DCDHF. High-functional apple pectin of low-moleculer oligosaccharide commercialized showed 26.6. The scavenging activity for ROO ・ was examined by DPPH. The high-functional apple pectin of low-moleculer oligosaccharide showed the value of 2.04, and it was the highest 4.47 on commercialized low-moleculer oligosaccharideAs future problem, it may be the completion of high-functional apple pectin of low-moleculer oligosaccharide to prevent a DNA damage. Also, it is necessary to examine the antioxydation power for 8-OHdG generation rate in the human and is regarded as contributing to the prevention of the lifestyle habit illness to people with many risk factors, by commercializing the high-functional apple pectin of low-moleculer oligosaccharide

通过将2000穗苹果果胶衍生物中的低聚糖按平均聚合度分为4组，检测其对DNA氧化损伤的预防效果。通过ESR研究，阐明了在低分子区的各个分级中，自由基清除作用都是高的。对该低分子量区的苹果果胶进行商品化尝试，研制出22.5%的D1、D2包合低分子量低聚糖。将低分子低聚糖的高功能性苹果果胶服用于人体，通过MC-FAOn对ONOO^-的清除活性，可以明确血流速度降低是如何改善血流动力学的，当使用DCDHF进行检测时，证明POS-S成分为2.4（滴度），加热的POS-S的POS-H为80.8。商品化的低分子低聚糖高功能苹果果胶含量为26.6。用DPPH法测定其对ROO ·的清除活性。高功能性低分子低聚糖苹果果胶的抗DNA损伤能力为2.04，是商品化低分子低聚糖中最高的4.47。此外，有必要检查对人体中8-OHdG生成率的抗氧化能力，并且认为通过将低分子低聚糖的高功能苹果果胶商业化，有助于预防具有许多危险因素的人的生活习惯病

项目成果

Tazawa K., Koike J., Namikawa H., Yatuzuka M et al.: "Characteristics on scavenging activity for free radic al in the Kampo medicine which suppresses hepatic met astasis"Kanpo-Medicine. 25. 15-19 (2001)

Tazawa K.、Koike J.、Namikawa H.、Yatuzuka M 等人：“抑制肝转移停滞的汉方药中自由基清除活性的特征”Kanpo-Medicine。

Tazawa K., Mizushima Y.: "Natural Apple Pectin-The mystery in the intestine by male and female"IRUKA BOOKS. 1-188 (2002)

田泽K.，水岛Y.：“天然苹果果胶-男性和女性肠道中的奥秘”IRUKA BOOKS。

田澤賢次: "汚れた腸をきれいにしてガンも活性酸素の害を防ぐアップルペクチンが豊富な焼きリンゴ"安心. 10. 40-42 (2003)

Kenji Tazawa：“富含苹果果胶的烤苹果，可以清洁肮脏的肠道，预防癌症和活性氧的危害” Anshin 10. 40-42 (2003)。

田澤賢次: "リンゴ繊維アップルペクチンが体重の増加を明らかに抑えることが判明"ゆほびか. 9. 66-67 (2001)

Kenji Tazawa：“苹果纤维苹果果胶明显抑制体重增加”Yuhobika。 9. 66-67 (2001)

田澤賢次, 水島豊: "天然アップルペクチン-男と女の腸内ミステリー"株式会社冬青社・イルカBOOKS. 1-188

田泽贤治、水岛裕：《天然苹果果胶-男女肠道之谜》Fuyuseisha Co., Ltd./Iruka BOOKS 1-188。