An analysis of inhibitory mechanism of colonic carcinoma and hepatic metastasis by apple pectin

高活性氧自由基可能在致癌和肝转移中发挥作用。在体内和体外研究了苹果果胶（低聚糖和低聚半乳糖醛酸）的自由基清除活性以及用于减弱结肠癌发生和肝转移的低聚糖成分。所使用的实验系统是通过电子自旋共振技术对超氧阴离子自由基和羟基自由基的自由基清除作用。苹果果胶低聚糖在体外表现出浓度依赖性的自由基清除活性，而在体内则不显着。低聚半乳糖醛酸的成分仅在最低分子量时显示出自由基清除活性。结果表明，经过处理的苹果果酱成分是良好的自由基清除剂，可能可以防止肝门系统的免疫抑制。

Highly reactive oxygen-derived free radicals may play a role in carcinogenesis and hepatic metastasis. Free radical scavenging activity of apple pectin (Oligosaccharides and Oligogalacturonic acids) and components of Oligosaccharides used for the attenuation of colon carcinogenesis and hepatic metastasis, were investigated in vivo and in vitro. The used exprimental system was a radical scavenging action against superoxide anion radical and hydroxyl radical by the electron spin resonance technique. Oligosaccharides of apple pectin showed radical scavenging activity in a concentration-dependent manner in vitro, while on the han there are not significant in vivo. Components of Oligogalacturonic acids showed radical scabenging activity only in lowest molecular weight. The results indicate that the components of apple pection treated for prevention of carcinogenesis and metastasis were good radical scabenger that could possibly prevent the immune suppression of hepato-portal system.