The Effect of Eluate from Composite Resin on Lymphoid cells.

复合树脂洗脱液对淋巴细胞的影响。

• 批准号: 62480386
• 负责人: TORII Mitsuo
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480386/
• 关键词: Composite resin; Eluate; マイトジェン活性; 溶出液; 免疫担当細胞

The mitogenic activity of eluate from a hardened composite resin (Photoclearfil A) for spleen cells derived from BALB/c mouse was investigated by using tritiated thymidine uptake. Resin discs (10 mm in diameter, 2mm in thickness) were immersed in RPMI-1640 medium (1 disc/5 ml) and the eluate was serially diluted by two fold. Dose-response was not established because the dispersion of data was very wide but both finally two and 16-fold diluted samples always showed clear mitogenic activity.It was attempted to specify the mitogenic substances which were included in the eluate from composite resin. The eluate to distilled water was fructionated by using high performance liquid chromatography (HPLC). Unreacted monomers Bis-GMA and 3G were confirmed to be included. Many other peaks were observed but we could not qualify them. Mitogenic activity of Bis-GMA and 3G were investigated. Both of these two materials induced good mitogenic responses on BALB/c mouse spleen cells.It was sugested that some of the mitogenic activity of eluate from composite resin depended on unreacted monomers Bis-GMA and 3G.

通过使用tri的胸苷摄取研究，研究了从BALB/C小鼠衍生的脾细胞中从硬化的复合树脂（Photoclearfil A）洗脱的有丝分裂活性。将树脂盘（直径为10 mm，厚度为2mm）浸入RPMI-1640培养基（1盘/5 mL）中，并通过两个折叠串行洗脱体。由于数据的分散非常宽，因此未建立剂量反应，但最后两个和16倍稀释的样品总是显示出明显的有丝分裂活性。通过使用高性能液相色谱（HPLC）来弥合蒸馏水到蒸馏水。未经反应的单体BIS-GMA和3G已被确认包括在内。观察到了许多其他峰，但我们无法鉴定它们。研究了BIS-GMA和3G的有丝分裂活性。这两种材料都在BALB/C小鼠脾细胞上诱导了良好的有丝分裂反应。这是对复合树脂中的一些促丝分裂活性的选择，取决于未反应的单体BIS-GMA和3G。

项目成果

鳥居光男: 日本歯科保存学雑誌.

Mitsuo Torii：日本保守牙科杂志。

Mitsuo, Torii: "The Mitogenic Activity of Eluate from Composite Resin." The Japanese Journal of Conservative Dentistry.

Mitsuo，Torii：“复合树脂洗出液的有丝分裂活性”。