Development of reversible antibacterial resin by ion-exchange mechanism.

利用离子交换机制开发可逆抗菌树脂。

基本信息

批准号：
08457505
负责人：
TORII Mitsuo
金额：
$ 4.67万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457505/
关键词：
antibacterial resin ion-exchange reversibility cationic antibacterial agent

项目摘要

The aim of this investigation is to give the long lasting antibacterial activity of agent release type to tooth coating material for caries prevention.Methactylic acid was added to triethylene glycol dimethacrylate as a cation-exchanger and photoactivated by camphorquinone and 2-dimethylaminoethyl methacrylate. A cationic antibacterial agent cetylpiridinium chloride (CPC) was dissolved to this test resin. The resin was photopolymerized to make disc shaped specimens (10 mm in diameter and 2 mm in thickness).The resin discs released CPC in buffers whose pHs were under 6.0, however, no release of CPC was observed in the pH over 6.5. The amount of CPC released increased as the pH of buffer decreased.The resin discs also released CPC when they were soaked in pH 7.0 phosphate buffer and sodium chloride was added to increase the ionic strength. The higher the ionic strength went up, the more the amount of CPC was released. When the resin, which finished to release CPC, was soaked in aqueous s … More olution of CPC, it recovered the ability of releasing CPC in acidic buffers. These results suggest that this resin works as a cation-exchange resin and retains CPC reversibly by ionic bond.The antibacterial activity of this resin was investigated in vitro and in vivo. At first S.mutans MT8148 was cultured in Brain Heart Infusion broth placed on the disc shaped specimen, but no viable cells were recovered. Next, the discs were suspended in sucrose containing HI broth inoculated with S.muttons MT8148 and cultured. After 24 hr culture, the bacterial cells adhered on the disc specimen were observed by SEM and the amount of collected cells were determined spectrophotomechally. Significantly less bacterial cells (1.7% of control resin specimen containing no CPC) were recovered and the anti-plaque activity was not influenced by saliva treatment. Finally, the antibacterial and anti-plaque activity was confirmed in vivo by using beagle dogs. The number of bacterial cells accumulated on the test resin was o.7% of that on control resin.The test resin reversibly retains CPC by ionic bond and releases CPC when the circumstance pH inclines to acidic. This mechanisms could provide dental resin with long lasting antibacterial activity of agent release type. The unique action of this test resin, releasing CPC only under acidic condition, would be ideal drug delivery system from the view point of caries etiology. Less

这项研究的目的是将剂类型的持续抗菌活性释放到预防牙齿的牙齿涂层材料中。将甲基酸性酸添加到三甲基乙二醇二甲基丙烯酸酯中，作为阳离子 - 脱甲基酯作为阳离子 - 脱甲基丙烯酸酯作为阳离子交换剂，并被樟脑甲基苯甲酸甲酯和2-二氨基甲基氨基甲基酰基甲基甲基甲酰基甲酯。将阳离子抗菌剂氯基（CPC）溶解在该测试树脂上。将树脂进行光聚合以使盘状样品（直径为10 mm，厚度为2 mm）。树脂盘以pHS低于6.0的缓冲液释放的CPC，但是，在6.5的pH值中未观察到CPC的释放。随着缓冲液的pH降低，释放的CPC量增加。树脂光盘还浸泡在pH 7.0磷酸盐缓冲液中时也释放了CPC，并添加氯化钠以提高离子强度。离子强度上升的越高，CPC的释放量就越大。当将CPC释放的树脂浸泡在水性中……更多的CPC溶液时，它恢复了在酸性缓冲液中释放CPC的能力。这些结果表明，该树脂可作为阳离子交换树脂，并通过离子键可逆地保留CPC。该树脂的抗菌活性在体外和体内研究了。首先，将S.Mutans MT8148培养在放置在椎间盘形样品上的脑心脏输液肉汤中，但没有回收活细胞。接下来，将圆盘悬挂在含有hi肉汤中的蔗糖中，接种了S.Muttons MT8148并进行了培养。 24小时培养后，通过SEM观察到粘附在椎间盘样本上的细菌细胞，并通过分光光学技术确定收集的细胞的量。发现细菌细胞（含有无CPC的对照树脂标本的1.7％）的细菌细胞被回收，抗扁平活性不受唾液处理的影响。最后，通过使用小猎犬犬在体内证实了抗菌和抗斑块活性。在对照树脂上积累的细菌细胞的数量为O.7％。测试树脂可可逆地通过离子键可逆地保留CPC，并在pH倾向于酸性时释放CPC。这种机制可以为牙齿树脂提供持续的抗菌活性，使其释放类型。该测试树脂的独特作用仅在酸性条件下释放CPC，这将是理想的药物递送系统，从龋齿病因的角度来看。较少的