Studies on the Induction of Immune Cells and Activating Mechanisms by Substances Derived from Oxoplasma Bgondii

本研究的目的是对弓形虫裂解抗原TLA和Obiactin的功能和结构进行分析，并分离和合成其功能成分。1.长春花素的功能。(1)。OBioactin对巨噬细胞内cAMP、cGMP、cGMP和pH水平无明显影响。(2)。Obiactin对小鼠脾细胞的杀伤活性无明显影响。2.语料库的功能。(1)。接种TLA或经TLA致敏的小鼠脾细胞可抑制小鼠移植瘤的增殖。(2)。Thy-1。TLA致敏小鼠脾组织中Lyt-1、Lyt-2、asialoGM1和Sig阳性细胞增多，胸腺Thy-1阳性细胞增多。(3)。当脾细胞与TLA共同培养时，可诱导出细胞毒细胞。抗asialoGM1或抗Thy-1血清可阻断细胞毒活性，但不能被抗Lyt-2血清阻断。(4)。当SPLE…之间的细胞间相互作用时可阻断更多NIC淋巴细胞和脾贴壁细胞，未诱导出细胞毒细胞。(5)。脾细胞与TLA共同培养的上清液不具有细胞毒活性和IL-2活性。(6)。TLA可刺激小鼠脾细胞的转化。3.卵母细胞肌动蛋白的纯化、合成及功能研究。(1)。将OBioactin的功能成分合成为活性多肽(BIOPPECTS)。胸腺肽原-α中含有相同的氨基酸序列。(2)。对弓形虫在巨噬细胞、单核细胞和心肌细胞中的生长有抑制作用。(3)。胞外多肽可促进巨噬细胞释放活性氧。(4)。生物肽对小鼠移植瘤的增殖有抑制作用。4.TLA活性部分的纯化、合成及其作用。(1)。从TLA中分离出生物活性多肽，合成了TLA-H6E。(2)。TLA-H6E与泛素的氨基酸序列同源性超过90%。(3)。将TLA-H6E与脾细胞共同培养，可诱导产生细胞毒细胞。较少

The purposes of this research project were the analysis of function and structure of Toxoplasma lysate antigen TLA) and Obioactin, and isolation and synthesis of functional components of them. 1. The Functions of obioactin. (1). The intracellular cAMP, cGMP, cGMP and pH levels of macrophages cultured with Obioactin did not changed. (2). Cytotoxic activities of splenocytes were not influenced by Obioactin. 2. The Function of TLA. (1). An inoculation of TLA or splenocytes of mice which were sensitized with TLA inhibited the multiplication of transplanted tumor in mice. (2). The Thy-1. Lyt-1, Lyt-2-, asialoGM1 and sIg positive cells in the spleen and Thy-1 positive cells in the thymus of mice which where sensitized with TLA increased. (3). When the splenocytes were cultured with TLA, cytotoxic cells were induced. And, the cytotoxic activity was blocked by treatment with anti-asialoGM1 or anti-Thy-1 serum, but not blocked by anti-Lyt-2 serum. (4). When cell to cell interaction between sple … More nic lymphocytes and splenic adherent cells was blocked any cytotoxic cells were not induced. (5). The supernatant which splenocytes were cultured with TLA did not show cytotoxic activity and IL-2 activity. (6). The blastogenesis of splenocytes was stimulated by TLA. 3. Purification and synthesis of Obioactin, and Function of Synthesized Obioactin. (1). The functional components of Obioactin was synthesized as active peptides (named obiopeptides). There is the same amino acid sequence obiopeptides in prothymosin-alpha. (2). Obiopeptides inhibited the growth of Toxoplasma gondii in macrophages, monocytes and heart cells. (3). Obiopeptides enhanced the release of the active oxygens from macrophages. (4). The multiplication of transplanted tumor in mice was inhibited by obiopeptides. 4. Purification and synthesis of active part of TLA, and action of synthesized TLA. (1). Biological active peptide was isolated from TLA, and TLA-H6E was synthesized. (2). The amino acid sequence of TLA-H6E and ubiquitin was similar more than 90 %. (3). When the splenocytes were cultured with TLA-H6E, cytotoxic cells were induced. Less