Study of The Heterogeneity in Cancer by Monoclonal Antibodies

用SHI（IgG_3）、FH_4（IgG_3）、FH_6（IgM）、AH_6（IgM）和TKH_2（IgM）单克隆抗体制备癌组织标本的连续切片，用免疫组化方法（ABC试剂盒）进行艾德染色，以检查染色区域。单个单克隆抗体的染色性质被认为是染色区域的差异，表明镶嵌现象。这一发现被认为是癌症异质性的一种表达模式。当使用单克隆抗体的组合时，在100%的癌组织标本中获得阳性结果。因此，已经发现借助于单克隆抗体的技术对于癌症的组织学诊断是有效的。也有人建议，阐明其异质性有助于选择适当的抗癌药物。

Serial sections of a cancer tissue specimen were prepared with a combination of monoclonal antibodies of different kinds-SHI (IgG_3), FH4(IgG_3), FH_6(IgM), AH_6(IgM), and TKH_2(IgM)-and stain ed with an immunohistochemical method(ABC kit) to examine stained areas. Staining properties of individual monoclonal antibodies were recognized as differences in stained areas, being indicative of mosaicism. This finding is considered one mode of expression of the heterogeneity of cancer.A positive result was obtained in 100% of the cancer tissue specimens when a combination of monoclonal antibodies was used. The technique by means of monoclonal antibodies has been thus found to be effective for the histological diagnosis of cancer. The suggestion has also been made that elucidating its heterogeneity aids in the selection of an appropriate anticancer drug.