Study of The Heterogeneity in Cancer by Monoclonal Antibodies

单克隆抗体对癌症异质性的研究

• 批准号: 63480312
• 负责人: NAKASAKI Hisao
• 金额: $ 3.71万
• 依托单位: Department of 2nd Surgery. Tokai University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480312/
• 关键词: Monoclonal Antibody; Mosaicism; Heterogeneity; モノクローナル抗体; 癌; 診断; 治療; モノクローナル複合体

Serial sections of a cancer tissue specimen were prepared with a combination of monoclonal antibodies of different kinds-SHI (IgG_3), FH4(IgG_3), FH_6(IgM), AH_6(IgM), and TKH_2(IgM)-and stain ed with an immunohistochemical method(ABC kit) to examine stained areas. Staining properties of individual monoclonal antibodies were recognized as differences in stained areas, being indicative of mosaicism. This finding is considered one mode of expression of the heterogeneity of cancer.A positive result was obtained in 100% of the cancer tissue specimens when a combination of monoclonal antibodies was used. The technique by means of monoclonal antibodies has been thus found to be effective for the histological diagnosis of cancer. The suggestion has also been made that elucidating its heterogeneity aids in the selection of an appropriate anticancer drug.

使用不同种类的单克隆抗体——SHI（IgG_3）、FH4（IgG_3）、FH_6（IgM）、AH_6（IgM）和TKH_2（IgM）的组合制备癌组织标本的连续切片，并用免疫组织化学方法（ABC试剂盒）染色以检查染色区域。各个单克隆抗体的染色特性被认为是染色区域的差异，表明嵌合体。这一发现被认为是癌症异质性的一种表达方式。当使用单克隆抗体组合时，100%的癌症组织标本获得了阳性结果。因此，已经发现利用单克隆抗体的技术对于癌症的组织学诊断是有效的。还有人建议阐明其异质性有助于选择合适的抗癌药物。

项目成果

中崎久雄: 日本外科学会雑誌.

中崎久雄：日本外科学会杂志。

Hisao Nakasaki: "Morsaicism in the-expression of Tunor-Associated carbohydrate Antigens in Human catonic and gastric Cancers" Cancer Research. 49. 3662-3669 (1989)

Hisao Nakasaki：“人类紧张性癌症和胃癌中肿瘤相关碳水化合物抗原表达中的摩尔赛现象”癌症研究。

中崎久雄: Cancer Research.

中崎久雄：癌症研究。

Hisao Nakasaki: "Moraicism in the exeression of Tumor-associatod carfohydrate Antigens in Human cabonic and Gtastric Cancers" Cancer Research. 49. 3662-3669 (1989)

Hisao Nakasaki：“人类癌症和胃癌中肿瘤相关碳水化合物抗原的表达中的摩尔现象”癌症研究。

Hisao Nakasaki et.al.: "Mosaicism in the Expression of Tumor associated Carbohydrate Antigens in Human Colonic and Gastric Cancers." Cancer Research. 49.3662-3669 July 1, 1989.

Hisao Nakasaki 等人：“人类结肠癌和胃癌中肿瘤相关碳水化合物抗原表达的镶嵌现象。”