New Therapeutic omega-3 unsaturated fatty acid for nonspecific inflammatory colonic diseases.

用于非特异性炎症性结肠疾病的新型治疗性 omega-3 不饱和脂肪酸。

• 批准号: 05807117
• 负责人: NAKASAKI Hisao
• 金额: $ 1.22万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807117/
• 关键词: non specific inflammatory clonic; disease; Crohn's disease; w-3 unsaturated fatty acid; trinitrobenzen sulfonic acid; EPA(エノコサペンタエン酸; 経腸栄養剤; 大腸炎症性疾患

The causes of nonspecific inflammatory diseases fo the intestinal tract--Crohn's disease, ulcerative colitis, and intestinal Behcet's disease--are unknown. The remedies for these afflictions are total parenteral nutrition and the administration of steroids and salazosulfa pyridine immunosuppressants. It is known, however, that total parenteral nutrition over a long period leads to a decline in local immunocompetence, accompanied by disused atrophy of the intestinal tract. This produces expansion of an auxiliary lesion fo the inflammatory diseases and delayd healing.The purposes of the research were as follow ;(1) (a) Preparatory development targeting the creation of an absorbable EPA,which has a superior antiinflammatory effect. (b) The establishment of preparatory technology for emulsification by the use of lecithin, and (c) the creation of an emulsion that includes w-3 fat by mixing soybean oil and fish oil. (2) Examination of the effectiveness of the pervenous-administration method, … More by using the newly developed fat nutrient on diseased-animals' models. (3) Confirmation of the effect as a fat nutrient (a) by administering an appropriate mixture of fish oil to animal models, (b) by analyzing the fatty acid in the serum, and (c) by examining the histological findings and the state of the biological function of the intestinal mucous membranes.The results :(1) The stable production of EPA (w-3 unsaturated fatty acid), the prevention of oxidization, and peroral pervenous administration became possible. (2)Creation of experimental animal models : Inflammatory colitis was created seven to ten days through anal postinjection of TNBS (2,4,6-trinitrobenzen sulfonic acid). (3) Remedial experiment using fish oil (w-3 unsaturated fatty acid)The experiment was conducted on Groups 1 through 5. After assessing the healing of large-intestinal ulcer through pathologic histological examination, significant effects were observed in Groups 2 and 3. No notable changes were observed in body weight, blood tests, etc. Less

肠道非特异性炎症性疾病——克罗恩病、溃疡性结肠炎和肠道白塞氏病——的病因尚不清楚。这些疾病的治疗方法是全肠外营养以及使用类固醇和柳氮磺胺吡啶免疫抑制剂。但众所周知，长期全胃肠外营养会导致局部免疫能力下降，并伴有肠道废弃性萎缩。这会导致炎症性疾病的辅助病变扩大并延迟愈合。本研究的目的如下：(1)(a)以创建具有优异抗炎作用的可吸收EPA为目标的前期开发。 (b)建立使用卵磷脂的乳化制备技术，以及(c)通过混合大豆油和鱼油来制备包含w-3脂肪的乳液。 (2) 通过在患病动物模型上使用新开发的脂肪营养素来检查静脉给药方法的有效性。 (3) 作为脂肪营养素的效果的确认 (a) 通过向动物模型施用适当的鱼油混合物，(b) 通过分析血清中的脂肪酸，以及 (c) 通过检查组织学结果和肠粘膜的生物功能状态。 结果：(1) EPA（w-3不饱和脂肪酸）的稳定产生、氧化的预防和口服 静脉给药成为可能。 (2)实验动物模型的制作：通过肛后注射TNBS(2,4,6-三硝基苯磺酸)7～10天制作炎症性结肠炎。 (3)鱼油(w-3不饱和脂肪酸)治疗实验在第1组至第5组进行实验。通过病理组织学检查评估大肠溃疡的愈合情况，第2组和第3组观察到显着效果。体重、血液检查等均未观察到明显变化。少