New Therapeutic omega-3 unsaturated fatty acid for nonspecific inflammatory colonic diseases.

肠道非特异性炎症性疾病--克罗恩病、溃疡性结肠炎和肠道白塞氏病--的病因尚不清楚。这些痛苦的补救措施是全胃肠外营养和类固醇和柳氮磺胺吡啶免疫抑制剂的管理。然而，众所周知，长期全胃肠外营养会导致局部免疫能力下降，并伴有肠道废用性萎缩。本研究的目的如下：（1）（a）以产生具有优良的上级止血效果的可吸收EPA为目标的预备性开发。(b)通过使用卵磷脂建立用于乳化的准备技术，以及（c）通过混合大豆油和鱼油产生包含w-3脂肪的乳液。(2)检查静脉给药方法的有效性， ...更多信息 通过在患病动物模型上使用新开发的脂肪营养素。(3)（a）通过将鱼油的适当混合物给予动物模型，（B）通过分析血清中的脂肪酸，（c）通过检查组织学发现和肠粘膜的生物功能状态，证实了作为脂肪营养素的效果。结果：（1）EPA（w-3不饱和脂肪酸）的稳定产生、氧化的防止和经口静脉给药成为可能。（2）实验动物模型的建立：采用TNBS（2，4，6-trinitrobenzensulfonate，2，4，6-三硝基苯磺酸）肛后注射7 ~ 10天建立炎症性结肠炎模型。(3)使用鱼油（w-3不饱和脂肪酸）的补救实验对第1组至第5组进行实验。通过病理组织学检查评估大肠溃疡愈合后，在第2组和第3组中观察到显著效果。在体重、血液检查等方面没有观察到显著变化。

The causes of nonspecific inflammatory diseases fo the intestinal tract--Crohn's disease, ulcerative colitis, and intestinal Behcet's disease--are unknown. The remedies for these afflictions are total parenteral nutrition and the administration of steroids and salazosulfa pyridine immunosuppressants. It is known, however, that total parenteral nutrition over a long period leads to a decline in local immunocompetence, accompanied by disused atrophy of the intestinal tract. This produces expansion of an auxiliary lesion fo the inflammatory diseases and delayd healing.The purposes of the research were as follow ;(1) (a) Preparatory development targeting the creation of an absorbable EPA,which has a superior antiinflammatory effect. (b) The establishment of preparatory technology for emulsification by the use of lecithin, and (c) the creation of an emulsion that includes w-3 fat by mixing soybean oil and fish oil. (2) Examination of the effectiveness of the pervenous-administration method, … More by using the newly developed fat nutrient on diseased-animals' models. (3) Confirmation of the effect as a fat nutrient (a) by administering an appropriate mixture of fish oil to animal models, (b) by analyzing the fatty acid in the serum, and (c) by examining the histological findings and the state of the biological function of the intestinal mucous membranes.The results :(1) The stable production of EPA (w-3 unsaturated fatty acid), the prevention of oxidization, and peroral pervenous administration became possible. (2)Creation of experimental animal models : Inflammatory colitis was created seven to ten days through anal postinjection of TNBS (2,4,6-trinitrobenzen sulfonic acid). (3) Remedial experiment using fish oil (w-3 unsaturated fatty acid)The experiment was conducted on Groups 1 through 5. After assessing the healing of large-intestinal ulcer through pathologic histological examination, significant effects were observed in Groups 2 and 3. No notable changes were observed in body weight, blood tests, etc. Less