New Therapeutic omega-3 unsaturated fatty acid for nonspecific inflammatory colonic diseases.

肠道非特异性炎症性疾病——克罗恩病、溃疡性结肠炎和肠白塞病的病因尚不清楚。治疗这些疾病的方法是全肠外营养和类固醇和萨拉唑磺胺吡啶免疫抑制剂的管理。然而，众所周知，长期的全肠外营养会导致局部免疫能力下降，并伴有肠道的废弃萎缩。这导致炎症性疾病的辅助病变扩大并延迟愈合。本研究目的如下：(1) (a)针对具有优越抗炎作用的可吸收EPA的制备的预备开发。(b)建立利用卵磷脂乳化的预备技术，(c)通过混合大豆油和鱼油制备含有w-3脂肪的乳液。(2)采用新研制的脂肪营养素对疾病动物模型进行静脉给药的有效性检验。(3)确认鱼油作为脂肪营养素的作用(a)通过给动物模型添加适当的鱼油混合物，(b)通过分析血清中的脂肪酸，(c)通过检查肠粘膜的组织学发现和生物学功能状态。结果：(1)使w-3不饱和脂肪酸（EPA）稳定生成、抗氧化、经口经静脉给药成为可能。(2)实验动物模型的建立：通过肛门注射TNBS（2,4,6-三硝基苯磺酸）后7 ~ 10天建立炎性结肠炎。(3)鱼油（w-3不饱和脂肪酸）治疗试验1 ~ 5组。经病理组织学检查评估大肠溃疡愈合情况，2、3组疗效显著。体重、血液检查等均未见明显变化。少

The causes of nonspecific inflammatory diseases fo the intestinal tract--Crohn's disease, ulcerative colitis, and intestinal Behcet's disease--are unknown. The remedies for these afflictions are total parenteral nutrition and the administration of steroids and salazosulfa pyridine immunosuppressants. It is known, however, that total parenteral nutrition over a long period leads to a decline in local immunocompetence, accompanied by disused atrophy of the intestinal tract. This produces expansion of an auxiliary lesion fo the inflammatory diseases and delayd healing.The purposes of the research were as follow ;(1) (a) Preparatory development targeting the creation of an absorbable EPA,which has a superior antiinflammatory effect. (b) The establishment of preparatory technology for emulsification by the use of lecithin, and (c) the creation of an emulsion that includes w-3 fat by mixing soybean oil and fish oil. (2) Examination of the effectiveness of the pervenous-administration method, … More by using the newly developed fat nutrient on diseased-animals' models. (3) Confirmation of the effect as a fat nutrient (a) by administering an appropriate mixture of fish oil to animal models, (b) by analyzing the fatty acid in the serum, and (c) by examining the histological findings and the state of the biological function of the intestinal mucous membranes.The results :(1) The stable production of EPA (w-3 unsaturated fatty acid), the prevention of oxidization, and peroral pervenous administration became possible. (2)Creation of experimental animal models : Inflammatory colitis was created seven to ten days through anal postinjection of TNBS (2,4,6-trinitrobenzen sulfonic acid). (3) Remedial experiment using fish oil (w-3 unsaturated fatty acid)The experiment was conducted on Groups 1 through 5. After assessing the healing of large-intestinal ulcer through pathologic histological examination, significant effects were observed in Groups 2 and 3. No notable changes were observed in body weight, blood tests, etc. Less