CULTURE OF CEREBRAL MICROVASCULAR ENDOTHELIAL CELL AND APPLICATION FOR THE ANALYSIS OF THE CEREBRAL ISCHEMIA

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(1) Cerebral endothelial cell damage induced by sodium arachidonate in vitroCytotoxic effect of sodium arachidonate on cultured microvascular endothelial cells derived from mongolian gerbil brain was measured by specific release of ^<51>Cr. Protective effect of cyclooxygenase and lipoxygenase inhibitors (indomethacin and nordihydroguaiaretic acid respectively) on cultured endothelial cells was also examined.Sodium arachidonate injured endothelial cells at the minimal concentration of 3mug/ml, and the cytotoxicity was dependent on the concentration of sodium arachidonate and incubation time. Nordihydroguaiaretic acid inhibited the endothelial injury by sodium arachidonate. On the other hand indomethacin had no protective effect on the endothelial cells. It is posturated that the endothelial cell injury was caused by lipoxygenase product.(2) Effect of arachidonate derivatives on cerebral endothelial permeability in vitroEndothelial cells derived from songolian gerbil brain were cultured on collagen coated membrane filter, and the effect of arachidonate derivatives on passage of FITC-albumin through endothelial cells was examined.Prostaglangin E_2 and F_2 alpha did not increase the permeability of endothelial cells. 15-HPETE increased the endothelial permeability slightly (p< 0.05). Leucotriene C_4 markedly increased the endothelial permeability (p<0.001). Leucotrienes may play an important role in the development of ischemic brain injury by increasing the cerebral microvascular endothelial permeability.